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Growth Parameters Treatments 1 2 3 4 5 6 Root length(cm) 31.24±10.27 35.00±3.78 44.74±6.66* 39.80±2.94 44.78±11.93* 42.56±7.42 Shoot height(cm) 84.46±25.00 132.92±8.87** 134.56±6.74** 140.54±5.88** 117.78±12.72* 124.06±21.24** Fresh biomass(g) 4.33±2.57 7.62±1.45 13.21±3.22** 14.69±3.58** 13.70±3.46** 14.57±1.57** Dry biomass(g) 1.82±1.42 5.04±1.17 6.41±1.64** 7.55±1.76** 7.56±1.85** 7.98±0.55** 1- control, 2- Penicillium chrysogenum Thom. 1, 3 - Bacterial isolate 5, 4 -both fungal and bacterial strains equal amount, 5- both fungal and bacterial isolates in 2:1 ratio, 6- both fungal and bacterial isolates in 1:2 ratio, (p > 0.05 *, p > 0.01 **) Table 1. Growth performance of Acacia nilotica grown under different microbial treatments (p > 0.05 *, p > 0.01 **)
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Shoot height (cm) Fresh biomass leaves (g) Dry biomass leaves (g) CONTROL 112.76±29.60 3.54±1.93 2.07±1.77 Penicillium grisefulvum 114.02±14.81 4.23±1.08 2.14±1.02 Penicillium restrictum 133.56±13.05 4.41±1.39 1.90±0.87 Penicillium crysogenum 137.58±8.59* 7.25±2.02 3.15±0.36* Aspergillus species 116.30±22.95 4.13±2.64 0.85±0.55 Aspergillus ornatus 119.42±20.45 11.76±4.94 1.80±0.11 Aspergillus wentii 122.72±17.30 1.42±0.57 4.57±2.87* Penicillum expansum 122.98±8.34 3.26±1.27 1.08±0.41 Paecilomyces variotii 121.70±13.22 9.82±6.02 1.35±0.34 Cunnighamella elegans 97.22±29.73 7.94±1.97 1.94±0.80 Bacterial isolate PB1 115.76±12.70 8.13±2.07 3.04±1.36 Bacterial isolate PB2 104.96±6.92 8.27±4.91 2.88±1.04 Bacterial isolate PB3 108.26±10.07 8.27±4.91 3.02±2.04 Bacterial isolate PB4 119.96±7.41 9.89±1.99 2.96±0.20 Bacterial isolate PB5 95.18±33.11 12.89±2.75* 4.13±0.93* Table 2. Effect of selected bioinoculant fungal and bacterial strains on growth of Acacia nilotica grown under pot culture conditions
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Treatment Shoot height (cm) Shootheight (cm) Root length (cm) Seedling height (cm) FBM leaf (g) DBM leaf (g) Collar dia (cm) FBM root (g) DBM root (g) C 1 70.94±6.62 19.58±1.44 90.52±7.44 8.14±1.31 3.79±0.67 18.60±2.70 2.75±0.18 1.47±0.13 C2 95.90±23.30** 30.96±3.31** 126.84±24.30** 10.27±1.50 5.29±0.67 21.20±1.92 2.62±0.41 1.37±0.29 C3 84.66±10.49 28.10±6.20* 112.76±14.56* 10.33±0.58 6.37±0.25* 20.22±2.12 2.49±0.25 1.37±0.21 R1 83.94±5.33 22.48±1.34 106.42±6.57 9.46±3.40 5.37±2.46 21.40±1.52 2.49±0.43 1.37±0.25 R2 108.02±9.29** 24.26±6.78 132.28±12.90** 10.32±1.39 6.59±0.48* 21.60±1.95 2.76±0.26 1.47±0.17 R3 99.04±9.43** 30.10±10.27** 129.14±17.55** 10.60±1.18 5.24±0.98 20.60±0.89 2.80±0.49 1.60±0.33 R4 118.72±6.58** 20.28±1.51 139.00±6.90** 9.84±1.60 6.00±1.41 20.60±0.89 3.11±0.38 1.69±0.18 R5 79.14±11.16 25.24±4.02 104.38±7.44 10.73±1.24 6.22±1.07 22.00±2.35 2.67±0.36 1.79±0.47 R6 71.90±7.74 31.12±5.84** 103.02±4.24 11.45±1.80** 6.14±1.05 19.00±2.55 3.15±0.33 1.74±0.45 R7 67.82±9.69 24.94±7.27 92.76±12.03 11.28±1.65** 7.10±1.04** 21.20±3.56 1.93±0.30 1.01±0.29 R8 65.46±9.69 28.24±3.32 93.70±10.35 9.14±1.45 4.61±1.41 21.80±3.70 2.68±0.54 1.49±0.42 R9 83.10±4.63 19.10±3.40 102.20±5.89 9.49±2.43 6.09±1.71 22.20±1.64* 2.78±0.35 1.41±0.22 R10 93.28±9.13* 35.56±5.82** 128.84±14.02** 10.65±1.43 5.50±0.94 20.80±2.39 3.37±0.55 1.62±0.29 R11 86.16±6.24 26.14±1.89 112.30±7.02* 12.06±2.20** 6.98±0.02** 21.00±1.41 2.50±0.87 1.38±0.36 C1– control uninoculated, C2– Penicillium chrysogenum Thom., C3– Bacterial isolate PB 5, R1-R11 –Rhizobial isolates (p>0.05 *, p>0.01 **) Table 3. Effects of selected microbial inoculants and Rhizobium on growth of Acacia nilotica
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Treatment Shoot height (cm) Root length (cm) Seedling height (cm) FBM leaf (g) DBM leaf (g) Collar dia (cm) FBM root (g) DBM root(g) PC+R1 51.04±7.59 20.22±1.12 71.26±8.46 11.96±2.29** 7.32±1.24** 21.60±1.67 2.97±0.40 1.55±0.15 PC+R2 89.48±12.31 33.82±8.67** 123.30±8.14** 11.38±3.04** 7.76±1.20** 20.80±1.92 2.61±0.25 1.46±0.20 PC+R3 80.42±12.41 33.98±6.84** 114.40±15.73** 11.18±1.31* 6.37±0.67* 22.00±2.35 2.69±0.26 1.59±0.25 PC+R4 60.04±7.58 44.48±2.30** 104.52±6.94 9.30±1.39 5.05±0.54 23.00±2.24** 3.32±0.31 1.71±0.22 PC+R5 79.40±7.60 18.38±1.21 97.78±6.53 7.91±2.71 3.28±1.22 22.20±2.59* 2.63±0.68 1.46±0.35 PC+R6 82.98±5.42 19.04±0.87 102.02±5.34 10.94±1.32* 5.39±0.79 21.60±3.78 2.47±0.59 1.34±0.47 PC+R7 59.90±10.03 20.26±1.00 80.14±9.31 9.91±0.78 4.97±0.70 21.20±1.79 2.69±0.61 1.64±0.23 PC+R8 80.04±11.14 23.72±2.18 103.72±9.62 10.91±1.03 5.89±0.55 20.80±2.17 2.62±0.60 1.31±0.41 PC+R9 56.26±6.78 32.28±2.53** 88.54±4.41 7.13±1.08 3.39±10 21.20±3.03 2.50±0.43 1.35±0.27 PC+R10 92.14±9.92* 32.64±2.02** 124.78±11.08** 9.72±1.89 6.08±0.98 21.60±1.34 3.84±1.38 2.16±0.95 PC+R11 99.10±6.57** 31.96±3.80** 131.06±6.41** 12.40±1.57** 6.30±0.12* 21.00±1.00 2.68±1.73 1.41±0.40 PC–Penicillium chrysogenum Thom. 1, R1-R11 –Rhizobial isolates (p>0.05 *, p>0.01 **) Table 4. Effects of dual inoculation of PC and Rhizobium on growth of Acacia nilotica
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Treatment Shoot height (cm) Root length (cm) Seedling height (cm) FBM leaf (g) DBM leaf (g) Collar dia (cm) FBM root (g) DBM root (g) PB5+R1 87.56±11.14 37.60±7.63** 125.16±16.69** 11.84±1.78** 6.31±0.21* 21.60±1.14 2.78±0.30 1.63±0.20 PB5+R2 91.18±26.56* 29.26±5.42** 120.44±30.17** 13.02±1.37** 6.90±1.51** 20.60±1.52 2.32±0.52 1.07±0.46 PB5+R3 5.66±12.72 19.74±1.13 75.40±12.66 11.08±1.19* 5.87±1.28 21.20±0.84 2.40±0.46 1.41±0.46 PB5+R4 60.68±6.91 25.40±3.45 86.08±3.51 11.51±1.02** 6.3±0.88* 20.80±0.84 2.11±0.36 1.2±0.22 PB5+R5 111.36±9.59** 30.30±7.07** 141.66±13.03* 12.99±1.73** 6.80±0.31* 21.00±1.22 2.74±0.27 1.46±0.17 PB5+R6 60.24±15.49 20.46±1.62 80.70±15.44 12.50±1.73** 5.78±1.28 21.20±0.84 3.38±0.72 1.78±0.40 PB5+R7 65.28±6.54 23.80±2.87 89.08±8.09 11.73±2.45** 7.47±0.36** 22.20±1.48* 2.74±0.20 1.51±0.14 PB5+R8 64.74±13.13 25.06±3.06 89.82±13.55 11.97±1.87** 6.11±0.73 21.80±2.17 2.85±0.23 1.63±0.08 PB5+R9 89.34±8.40 30.64±4.27** 119.98±11.00** 10.68±2.68 6.61±2.19* 22.60±1.67* 2.57±0.13 1.42±0.08 PB5+R10 85.86±8.51 31.54±2.16** 117.40±9.64** 11.32±2.37 5.72±1.52 22.60±1.67* 2.72±0.38 1.61±0.29 PB5+R11 101.70±12.19** 35.92±6.31** 137.62±13.87** 11.08±2.14 6.58±1.34* 21.20±1.64 3.29±0.42 1.82±0.41 PB5 Bacterial isolate, R1-R11 –Rhizobial isolates (p>0.05 *, p>0.01 **) Table 5. Effects of dual inoculation of PB5 and Rhizobium on growth of Acacia nilotica
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Treatment Shoot height (cm) Root length (cm) Seedling height (cm) FBM leaf (g) DBM leaf (g) Collar dia (cm) FBM root (g) DBM root (g) CONTROL 70.94±6.62 19.58±1.44 90.52±7.44 8.14±1.31 3.79±0.67 18.60±2.70 2.75±0.18 1.47±0.13 PC+PB5+R1 100.80±11.17** 31.64±5.48** 132.44±14.34** 10.80±1.21 6.06±0.72 21.20±1.64 3.09±0.35 1.68±0.18 PC+PB5+R2 68.04±7.98 25.36±4.23 93.40±9.14 11.33±1.82 5.65±1.40 21.60±0.89 2.56±0.60 1.55±0.43 PC+PB5+R3 81.40±23.42 27.12±5.73 108.52±23.97 11.34±1.20 6.79±0.68* 20.40±0.55 3.02±0.18 1.79±0.20 PC+PB5+R4 89.96±10.45 21.96±1.73 111.92±9.48* 11.01±1.60 6.37±0.27* 21.20±2039 2.78±0.29 1.44±0.12 PC+PB5+R5 98.64±10.25** 36.94±3.75 135.58±11.99** 11.97±2.38 6.21±1.07 21.00±2.55 3.81±0.57 2.05±0.39 PC+PB5+R6 75.66±5.62 32.04±2.05** 107.70±6.73 10.92±1.82 6.26±0.64* 21.60±2.41 3.02±0.78 1.81±0.37 PC+PB5+R7 90.64±12.61** 32.22±3.64** 122.86±12.45** 11.27±2.33 7.20±1.12** 23.60±0.55** 2.96±0.37 1.63±0.15 PC+PB5+R8 78.60±13.66 31.40±2.76** 110.00±11.23 11.88±1.35 6.70±0.38* 22.20±1.79* 2.64±0.48 1.39±0.26 PC+PB5+R9 75.56±10.08 37.08±3.42** 112.64±8.00* 11.59±2.16 6.78±1.31* 20.80±1.92 2.74±0.58 1.46±0.33 PC+PB5+R10 83.80±5.77 42.06±1.93** 125.86±4.31** 13.44±2.45 6.96±1.96** 21.60±2.88 2.96±0.50 1.61±0.18 PC+PB5+R11 73.40±6.02 40.00±6.64** 123.40±3.81** 13.85±1.11 7.69±0.76** 23.60±1.52** 3.28±0.48 1.72±0.28 C– control un- inoculated, PC- Penicillium chrysogenum Thom. 1, PB5– Bacterial isolate, R1-R11 –Rhizobial isolates (p>0.05 *, p>0.01 **) Table 6. Effects of Triple inoculation of Pencillium chrysogenum, PB 5 and Rhizobium on growth of Acacia nilotica
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