Optimization of Mycelial Culture Conditions and Fructification of <i>Lentinus</i> Species Using Rice Straw and Sawdust Based Substrates

SP Kalaw; RMR Dulay; EJ Damaso; JC Ramos; MAG del Rosario; MD Abon; AM De Leon; JR Undan; RG Reyes; SP Kalaw; RMR Dulay; EJ Damaso; JC Ramos; MAG del Rosario; MD Abon; AM De Leon; JR Undan; RG Reyes

doi:10.5943/sif/6/1/42

Figures (1) Tables (2)

Figure 1. Fruit bodies of Lentinusspecies/strains growing on fruiting bags containing 7:3 rice straw– sawdust substrate formulation. a L. sajor-caju strain 1. b L. sajor-caju strain 2. c L. squarrosulus strain 1. d L. squarrosulus strain 2. e L. strigosus strain 1. f L. strigosus strain 2. g L. swartzii.

Factors	Mycelial growth rate (day^-1)
Culture media	L. sc strain1	L. sc strain 2	L. sq strain1	L. sq strain2	L. st strain 1	L. st strain 2	L. sw
CWG	12.75 ± 0.18^a	18.00 ± 0.00^a	12.86 ± 0.00^a	12.84 ± 0.02^a	15.00 ± 0.00^a	13.87 ± 1.97^a	18.00 ± 0.00^a
PSG	8.37 ± 0.08^cd	17.22 ± 0.28^ab	10.37 ± 0.10^b	4.27 ± 0.26^e	15.00 ±0.00^a	15.00 ±0.00^a	18.00 ±0.00^a
CGDG	6.80 ± 1.55^d	15.85 ± 0.75^bc	9.57 ± 0.75^bc	8.70 ± 0.05^c	13.80 ± 0.84^ab	14.81 ± 0.33^a	18.00 ± 0.00^a
RBDG	8.79 ± 0.06^c	17.22 ±0.76^ab	12.86 ±0.00^a	8.44 ± 0.81^cd	13.20 ±0.07^b	15.00 ±0.00^a	17.23 ±0.27^b
PDA	10.25 ±0.61^c	17.61 ±0.03^a	6.89 ± 0.24^d	7.30 ± 0.76^d	14.11 ±0.35^ab	14.56 ±0.13^a	16.27 ±0.41^c
MEA	11.46 ± 0.56^ab	14.88 ± 0.67^c	6.05 ± 0.20^d	11.20 ± 0.33^b	11.87 ± 0.74^c	14.49 ± 0.49^a	18.00 ± 0.16^a
SDA	10.91 ±0.59^ab	17.99 ±0.42^a	9.19 ± 0.19^c	10.14 ±0.25^b	14.66 ±0.17^a	14.93 ±0.88^a	8.00 ± 0.00^e
MA	8.78 ± 0.61^c	17.83 ±0.29^a	4.43 ± 0.19^e	5.17 ± 0.12^e	9.44 ± 0.43^d	15.00 ±0.00^a	11.81 ±0.12^d
pH
5.0	18.00 ± 0.00^a	18.00 ± 0.00^a	12.49 ± 0.63^a	12.86 ± 0.00^a	15.00 ± 0.00^a	13.87 ± 0.14^c	17.86 ± 0.24^a
5.5	17.66 ± 0.23^a	18.00 ± 0.00^a	11.89 ± 0.10^a	12.86 ± 0.00^a	13.86 ± 0.14^abc	14.21 ± 0.36^bc	17.91 ± 0.16^a
6.0	16.65 ± 0.09^b	17.53 ± 0.43^a	11.37 ± 0.15^a	12.86 ± 0.00^a	13.17 ± 0.21^bcd	14.63 ± 0.39^ab	18.00 ± 0.00^a
6.5	16.02 ± 0.07^c	17.24 ± 0.43^a	7.99 ± 0.62^b	12.86 ± 0.00^a	12.87 ± 0.04^cd	15.00 ± 0.00^a	18.00 ± 0.00^a
7.0	14.82 ± 0.29^d	16.13 ± 0.43^b	8.20 ± 0.66^b	12.86 ± 0.00^a	14.19 ± 0.23^ab	15.00 ± 0.00^a	18.00 ± 0.00^a
7.5	14.11 ± 0.13^e	15.26 ± 0.65^b	8.44 ± 0.22^b	11.89 ± 0.28^b	12.53 ± 0.06^d	15.00 ± 0.00^a	18.00 ± 0.00^a
8.0	13.50 ± 0.18^f	15.14 ± 0.09^b	7.84 ± 0.21^b	12.19 ± 0.04^b	12.51 ± 1.18^d	13.59 ± 0.28^c	18.00 ± 0.00^a
Aeration
Sealed	18.00 ± 0.00^a	18.00 ± 0.00^a	15.00 ± 0.00^a	12.86 ± 0.00^a	14.59 ± 0.37^a	15.00 ± 0.00^a	18.00 ± 0.00^a
Unsealed	18.00 ± 0.00^a	18.00 ± 0.00^a	13.78 ± 0.13^b	12.86 ± 0.16^a	15.00 ± 0.00^a	15.00 ± 0.00^a	18.00 ± 0.00^a
Illumination
Lighted	18.00 ± 0.00^a	18.00 ± 0.00^a	15.00 ± 0.84^a	15.00 ± 0.00^a	15.00 ± 0.00^a	15.00 ± 0.00^a	18.00 ± 0.00^a
Dark	18.00 ± 0.00^a	18.00 ± 0.00^a	13.10 ± 0.00^b	14.58 ± 0.00^b	13.10 ± 0.99^b	15.00 ± 0.00^a	18.00 ± 0.00^a
Temperature
9℃	ng	ng	ng	ng	ng	ng	ng
23℃	18.00 ± 0.00^a	20.31 ± 0.24^b	15.00 ± 0.00^a	14.58 ± 0.14^b	13.19 ± 0.11^b	13.98 ± 0.19^b	16.86 ± 0.24^b
30℃	18.00 ± 0.00^a	22.50 ± 0.00^a	15.00 ± 0.00^a	15.00 ± 0.00^a	15.00 ± 0.00^a	15.00 ± 0.00^a	18.00 ± 0.00^a
Means with similar superscripts are statistically comparable from each other using Tukeys HSD and t-test at 5% level of significance. L. sc, Lentinus sajor-caju; L. sq, Lentinus squarrosulus; L. st, Lentinus strigosus; L. sw, Lentinus swartzii; CWG, coconut water gulaman; PSG, potato sucrose gulaman; CGDG, corn grit decoction gulaman; RBDG, rice bran decoction gulaman; PDA, potato dextrose agar; MEA, malt extract agar; SDA, Sabouraud dextrose agar; MA, mycological agar; ng, no growth.

Table 1. Effect of culture media, pH, aeration, illumination and temperature on the mycelial growth of the seven Lentinus strains

*Lentinus*	Incubation period (day)	Primordia initiation (day)	Cap diameter (mm)	Stipe length (mm)	Yield (g bag^-1)	Biological efficiency (%)
L. sc strain 1	20.9 ± 0.7	24.0 ± 0.8	60.7 ± 6.6	14.5 ± 1.3	44.2 ± 6.7	8.8 ± 1.3
L. sc strain 2	21.3 ± 1.1	24.5 ± 1.1	62.7 ± 4.4	14.8 ± 1.6	50.9 ± 6.3	10.2 ±1.3
L. sq strain 1	25.6 ± 2.3	31.9 ± 2.8	45.8 ± 7.6	53.5 ±8.3^*	36.4 ±10.9^*	7.3 ±2.2^*
L. sq strain 2	25.5 ± 2.6	33.5 ± 2.9	45.5 ± 6.8	8.1 ± 1.9	23.5 ± 8.6	4.7 ±1.7
L. st strain 1	18.0 ± 0.8	21.6 ± 0.5	28.5 ± 5.1	19.6 ± 1.9	52.5±12.7^*	10.5 ±2.6^*
L. st strain 2	18.8 ± 2.8	22.3 ± 2.9	51.9 ± 9.3^*	19.7 ± 2.6	21.1 ±7.4	4.2 ±1.5
L. sw	18.4 ± 1.4	22.0 ± 1.6	40.6 ±1.71	36.5±3.3	34.6 ±5.9	6.9 ±1.2
Asterisk () indicates significant difference between two strains of each Lentinus* species (except L. swartzii) using t-test at 5% level of significance. L. sc, Lentinus sajor–caju; L. sq, Lentinus squarrosulus; L. st, Lentinus strigosus; L. sw, Lentinus swartzii.

Table 2. Fructification parameters of the seven strains of Lentinus