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Figure 1. 

pH of in vitro reactions. All measurements were expressed as the mean ± SE. a–c: Different letters at the same time points are significantly different between treatments (p < 0.05). x–z: Different letters are significantly different at different incubation times within the same treatment (p < 0.05). (n = 6).

Figure 2. 

The content of lactate in the in vitro reactions. All measurements were expressed as the mean ± SE. a–c: Different letters at the same time points are significantly different between treatments (p < 0.05). x–z: Different letters are significantly different at different incubation times within the same treatments (p < 0.05). (n = 6).

Figure 3. 

The content of glycogen in the in vitro reactions. All measurements were expressed as the mean ± SE. a–c: Different letters at the same time points are significantly different between treatments (p < 0.05). x–z: Different letters are significantly different at different incubation times within the same treatments (p < 0.05). (n = 6).

Figure 4. 

The content of glucose-6-phosphate in the in vitro model. All measurements were expressed as the mean ± SE. a–c: Different letters at the same time points are significantly different between treatments (p < 0.05). x–z: Different letters are significantly different at different incubation times within the same treatments (p < 0.05). (n = 6).

Figure 5. 

The activities of glycolytic enzymes in the in vitro model at 0.5 h. All measurements were expressed as the mean ± SE. a–c: Different letters at the same pH conditions are significantly different between treatments (p < 0.05). x–z: Different letters are significantly different at different incubation pH within the same treatments (p < 0.05). (n = 6).

Figure 6. 

The expression value of glycolytic enzymes in the in vitro model at 0.5 h. All measurements were expressed as the mean ± SE. a–c: Different letters are significantly different between treatments (p < 0.05). (n = 6).

Figure 7. 

The S-nitrosylation levels of glycolytic enzymes in the in vitro reactions at 0.5 h. All measurements were expressed as the mean ± SE. a–c: Different letters are significantly different between treatments (p < 0.05). (n = 6).