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Figure 1.
Characteristics of single and semi-double flower carnations. (a) 'Peach Party' (single flower, pink), (b) 'Pink Star' (semi-double flower, pink), (c) 'Purple Star' (semi-double flower, purple), (d) 'Oscar' (semi-double flower, red). Scale bars = 1 cm.
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Figure 2.
Sequence polymorphisms of A-class and C-class genes in SF and SDF cultivars. (a) Amino acid substitutions in AGa. (b) Amino acid substitutions in AGb. (c) Sequence covering miR172 bind site of AP2.
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Figure 3.
Expression analysis of ABCE model genes in different floral organs.
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Figure 4.
The regulation network of flower formation in carnation.
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Flower type Accessions Sepal
numberPetal
numberStamen
numberSingle Peach Party 5.00 ± 0.00 5.00 ± 0.00 10.00 ± 0.00 Semi-double Pink Star 5.00 ± 0.00 29.00 ± 3.30 5.20 ± 2.30 Semi-double Purple Star 5.00 ± 0.00 29.20 ± 3.33 4.90 ± 3.14 Semi-Double Oscar 5.00 ± 0.00 26.00 ± 2.79 4.30 ± 2.06 Table 1.
Statistics of floral organs of the four cultivars.
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Gene Application Forward primer Reverse primer AGa cloning ATGGAATTTTCAAGCCAAATAACTAGG CCAAACACCTCTTCAACTTGTTTGA AGb cloning ATGGAGTTTTCAAGCCAAATTAC AAACTCCTCTCCAACTTGTGTAA AP2 cloning TGGTACGCCTGATGAAACGAA TGCCCCCTAATGGTTTCCAC DcUbq3–7 RT-qPCR GTTGTTGGTTTCAGGGCTGGTTTG CTACGGTAATTGAGAATTCACACCGAAATG AGa RT-qPCR ATGCTAATCATAGCGTGAAGG GTTGGCTTCGGCAACAGA AGb RT-qPCR CCTCAAGCCAAAGGAAGCTA ACCCATTTCTTCTCTTGCAG AP1 RT-qPCR TAGGTCAAGATTTGGATACGCT ATCTAATGTGTTTGAGGCCG AP2 RT-qPCR CGCGTATGGGTCAATTTCT AATTAGTAACCGCATCCTTCC AP3 RT-qPCR GTCTGCTCGCTCTCAGATT GTAAGTCGTGACACACGAT PI RT-qPCR CTTCGGTTGAAGAAATCCTAGA GGCTGAGATTTTCATGTTTTGC SEP1 RT-qPCR GCAGCAAACATGGGAAGG GTCAATGGGCTGGAAAAGAG SEP3 RT-qPCR TGATAGAAGCAAATCAAGCGAC GTGAAAGAAGACATGGTCTCC Table 2.
Primer sequence for gene cloning and expression analysis used in this study.
Figures
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Tables
(2)