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    • Figure 1. 

      Analysis of (a) H3ac and H4ac protein content, (b) CsTSB2 expression level and (c) indole content after HDAC inhibitor treatment in tea plants. (a) Detection of H3ac and H4ac content after combinatorial histone deacetylases inhibitor feeding for 24 h. CK, methanol-water (1/19, v/v) treatment group. Inhibitor, combinatorial histone deacetylases inhibitor treatment group. (b) Detection of CsTSB2 expression level and (c) indole content after HDAC inhibitor treatment. 24 h: incubate for 24 h with HDACs inhibitor or methanol/water; 24 + 3 h: After incubating with HDACs inhibitor or methanol/water for 24 h, perform mechanical wounding treatment for 3 h; 24 + 6 h: After incubating with HDACs inhibitor or methanol/water for 24 h, perform mechanical wounding treatment for 6 h. Internal reference gene: CsEF1-α. Data are expressed as mean ± S. D. (n = 3). Significant differences between control and treatment at the same different treatment time are indicated (* p ≤ 0.05, and ** p ≤ 0.01).

    • Figure 2. 

      ChIP-qPCR analysis of (b) histone 3 acetylation (H3ac) and (c) histone 4 acetylation (H4ac) enrichment of CsTSB2 promoter after wounding treatments. (a) The precise location of primers used for ChIP-qPCR are indicated. CK: No-treatment at 3 h; T: Continuous wounding treatment at 3 h; TSB, Tryptophan synthase β-subunit; P, Promoter. Anti-IgG antibody was used as a negative control. The 'number X' represents the difference multiplier between the two groups. Data are expressed as mean ± S. D. (n = 3).

    • Figure 3. 

      Changes in expression levels of CsHDACs in tea leaves exposed to wounding treatment. HDA: histone deacetylase-A; SRT: sirtuin; HDT: histone deacetylase. Internal reference gene: CsEF1-α. Significant difference between control and wounding treatment are indicated (* p ≤ 0.05, and ** p ≤ 0.01). Data were expressed as mean ± S. D. (n = 3).

    • Figure 4. 

      The subcellular localization and binding level of CsHDA8. (a) The subcellular localization and binding level of CsHDA8 in Arabidopsis thaliana protoplasts. CsHDA8 and nuclear-localized mcherry are co-transferred into A. thaliana protoplasts. (b) ChIP-qPCR detection the binding level of CsHDA8 to CsTSB2 promoter after mechanical wounding treatments in tea plant. CK: No-treatment at 3 h; T: Continuous wounding treatment at 3 h. Anti-IgG antibody was used as a negative control. The specific positions represented by P1, P2, P3, and P4 in the figure are shown in Fig. 2a. The 'number X' represents the difference multiplier between the two groups. Data are expressed as mean ± S. D. (n = 3). HDA8, histone deacetylase 8. TSB, Tryptophan synthase β-subunit.

    • Figure 5. 

      Mechanism of CsHDA8 involvement in regulating the synthesis of indole induced by mechanical wounding during the processing of oolong tea. H3ac, Histone H3 acetylation; H4ac, Histone H4 acetylation. ×, Repress expression.