Figures (3)  Tables (3)

    • Figure 1. 

      Associations of the DNA quantity or 'Candidatus Liberiabcter asiaticus' titers with the weight of (a) leaf midrib or (b) bark tissue from sour tangerine (Citrus sunki Hort. ex Tanaka) seedlings.

    • Figure 2. 

      Different growth status of buds carrying 'Candidatus Liberibacter asiaticus' used for graft inoculation (a) and percentage stacking of the number of months that plants with different buds were positive for CLas (b). (a1) The 'Candidatus Liberibacter asiaticus' (CLas)-infected donor buds were in vigorous growth after grafting. A total of 30 stock plants were with 'a-bud' . (a2) The buds were in moderate growth condition after grafting. A total of 36 acceptor plants were with 'b-bud'. (a3) The buds with poor growth status on stock seedlings after grafting. A total of 26 stock seedlings were with 'c-bud'.

    • Figure 3. 

      Schematic diagram of (a) side grafting, (b) T grafting and (c) V grafting.

    • Sample weight (g)DNA concentration
      (ng/µL)      		CtNo of CLas per ng DNANo of CLas per g sample and per ng DNA
      0.1000346.80 ± 8.55a20.59 ± 0.27437.99 ± 0.18a4379.88 ± 1.78c
      0.0500302.32 ± 32.65a20.81 ± 0.18327.38 ± 14.86b6547.54 ± 297.13c
      0.0250209.01 ± 9.05b20.99 ± 0.09455.11 ± 11.93a18204.47 ± 477.17b
      0.012593.24 ± 13.42c21.98 ± 0.23443.26 ± 28.64a35460.98 ± 2291.10a
      0.006367.27 ± 4.58c24.88 ± 1.2541.52 ± 11.59c6591.01 ± 1839.16c
      0.1000569.70 ± 4.63a25.52 ± 0.714.79 ± 1.55d47.89 ± 15.48c
      0.0500410.17 ± 24.84b21.51 ± 0.04161.35 ± 6.90c3227.05 ± 138.04b
      0.0250255.51 ± 17.53c21.76 ± 0.15218.51 ± 13.48b8740.35 ± 539.30b
      0.0125133.12 ± 6.73d22.66 ± 0.69360.91 ± 9.14a28873.06 ± 731.36a
      0.006366.78 ± 2.89e24.21 ± 0.16154.34 ± 21.75c24498.65 ± 3452.89a
      Data in the table are showed as mean ± standard error (SE). Data of the first five lines are for leaf midrib samples, while the later five lines are for the shoot bark samples. The statistic analysis was carried out separately between sample weight and each of the six groups of relative index separately.

      Table 1. 

      DNA quantity and 'Candidatus Liberibcter asiaticus titer' titers of the samples from leaf midrib or bark tissues of sour tangerine (Citrus sunki Hort. Ex Tanaka) seedlings.

    • TimeGrafting method
      Side grafting'T' grafting'V' grafting
      4 MAGSuccess rate16.7%ab41.7%a16.7%ab
      CLas concentration*35.71 ± 25.83269.40 ± 97.35295.61 ± 168.07
      Average Ct25.65 ± 0.9623.21 ± 0.3922.78 ± 0.88
      5 MAGSuccess rate25.0%bc83.3%a41.7%b
      CLas concentration*171.51 ± 138.89139.30 ± 28.85135.07 ± 29.65
      Average Ct25.03 ± 0.9523.00 ± 0.2322.76 ± 0.43
      6 MAGSuccess rate33.3%c83.3%a33.3%c
      CLas concentration*78.29 ± 18.67213.69 ± 74.35232.99 ± 78.33
      Average Ct23.67 ± 0.3924.66 ± 0.6423.31 ± 0.22
      7 MAGSuccess rate33.3%bc83.3%a41.7%b
      CLas concentration*107.38 ± 28.00164.86 ± 29.64266.92 ± 76.64
      Average Ct23.72 ± 0.5323.78 ± 0.5524.21 ± 0.53
      8 MAGSuccess rate33.3%bc83.3%a41.7%b
      CLas concentration*204.82 ± 63.54111.26 ± 18.7283.67 ± 28.61
      Average Ct25.53 ± 1.4825.25 ± 0.3826.18 ± 0.54
      MAG, Month-after-grafting. Statistic analysis was done horizontally in the table, indicating the differences among different grafting methods.
      * The unit of CLas concentration is copy number per ng DNA. The significance analysis was performed with letter markers such as a, b, and c. The difference is not significant if the marked letters are the same, and vice versa.

      Table 2. 

      The successful transmission rate and effect of different grafting methods.

    • TimeNumbers of buds
      One budTwo budsThree buds
      4 MAGSuccess rate13.3%ab33.3%a33.3%a
      CLas concentration*35.71 ± 25.8345.81 ± 14.51231.58 ± 147.16
      Average Ct25.65 ± 0.9625.19 ± 0.5925.29 ± 1.74
      5 MAGSuccess rate20.0%b80.0%a66.7%a
      CLas concentration*171.51 ± 138.89153.61 ± 39.5793.99 ± 16.63
      Average Ct25.03 ± 0.9524.30 ± 0.4824.39 ± 0.53
      6 MAGSuccess rate26.7%bc73.3%a53.3%ab
      CLas concentration*78.29 ± 18.67206.20 ± 37.25158.53 ± 38.07
      Average Ct23.67 ± 0.3924.62 ± 0.2625.91 ± 0.69
      7 MAGSuccess rate26.7%c93.3%a66.7%b
      CLas concentration*107.38 ± 28.00180.34 ± 36.6461.94 ± 12.32
      Average Ct23.72 ± 0.5323.25 ± 0.5124.19 ± 0.36
      8 MAGSuccess rate26.7%c93.3%a66.7%b
      CLas concentration*204.82 ± 63.5468.42 ± 14.0755.99 ± 18.69
      Average Ct25.53 ± 1.4826.52 ± 0.3826.46 ± 0.51
      MAG, Month-after-grafting. Statistic analysis was carried out horizontally in the table, indicating the differences among different number of buds.
      * The unit of CLas concentration is copy number per ng DNA.

      Table 3. 

      The successful transmission rate and effect of the numbers of grafting buds on HLB transmission by grafting.