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Figure 1.
Metabolite (compounds with variable importance in projection > 1) variations during enzyme reaction stage analyzed by LC/MS and GC/MS. (a) These non-volatile metabolites accumulate gradually in the enzymatic reaction stage. (b) These non-volatile metabolites decrease gradually in the enzymatic reaction stage. (c) The score scatter plots of PLS-DA of volatile metabolites. (d) Validation of the PLS-DA model. (e) Heatmap of differential volatile substances during enzyme reaction stage. VIP: variable importance projection, GA: gallic acid, EGC:(–)-epigallocatachin, ECG: (–)-epicatechin gallate, CG: (–)-catechin gallate, EC: (–)-epicatechin. Different lower case letters following the number indicate significant differences during the processing (p < 0.05). * Represents compounds that have not been validated by available standards.
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Figure 2.
Metabolite (compounds with variable importance in projection > 1) variations during nonenzymatic reaction stage analyzed by LC/MS and GC/MS. (a) These non-volatile metabolites accumulate gradually in the nonenzymatic reaction stage. (b) These non-volatile metabolites decrease gradually in the nonenzymatic reaction stage. (c) The score scatter plots of PLS-DA of volatile metabolites. (d) Validation of the PLS-DA model. (e) Heatmap of differential volatile substances during nonenzymatic reaction stage. VIP: variable importance projection, EGCG: (–)-epigallocatechin gallate, EGC: (–)-epigallocatechin. Different lower case letters following the number indicate significant differences during the processing (p < 0.05). * Represents compounds that have not been validated by available standards.
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Figure 3.
Dynamic variation of major metabolites. DMAPP: dimethylallyl pyrophosphate, PEP: phosphoenolpyuvate, E4P: erythrose 4-phosphate, FPP: farnesyl pyrophosphate, GPP: geranyl pyrophosphate, IPP: isopentenyl pyrophosphate, Phe: phenylalanine, IGP: indole-3-glycerol phosphate, GLVs: green leaf volatiles, the methylerythritol phosphate (MEP) pathway, the mevalonic (MVA) acid pathway, lipoxgenase (LOX) pathway.
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Figure 4.
Dynamic variation of major compounds between JX and TGY during processing. (a) Dynamic changes in the content of major non-volatile metabolites in JX varieties. (b) Dynamic changes in the content of major non-volatile metabolites in TGY varieties. (c) Dynamic changes in the content of major volatile metabolites in JX varieties. (d) Dynamic changes in the content of major volatile metabolites in TGY varieties. GLVs: green leaf volatiles (hexanal, (E)-2-hexenal, cis-3-hexen-1-ol,1-hexanol, cis-3-hexenyl acetate); homoterpenes ((3E)-4,8-dimethyl-1,3,7-nonatriene); monoterpenes (β-myecene, D-limonene, β-trans-ocimene, cis-β-ocimene, linalool, hotrienol, trans-linalool 3,7-oxide, β-cyclocitral, cis-geraniol, and citral); sesquiterpenes (α-cubebene, β-bourbenene, β-cubebene, caryophyllene, α-farnesene, δ-Cadinene, nerolidol, cubenol); JX: Jinxuan, TGY: Tieguanyin.
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Figure 5.
The changes of taste and aroma metabolites in Oriental Beauty during the whole production process. PPO: polyphenol oxidase, POD: peroxidase, GA: gallic acid, MEP pathway: the methylerythritol phosphate pathway.
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Compounds
(mg/g)FTL Wi-OB 1S-OB 2S-OB 3S-OB 4S-OB 5S-OB ST-OB FX-OB OB GC 8.730 ± 1.539a 6.874 ± 0.341b 6.394 ± 0.386b 4.147 ± 0.363c 3.829 ± 1.028c 2.941 ± 0.754d 1.445 ± 0.677e 0.670 ± 0.514f 0.927 ± 0.453ef 1.126 ± 0.341ef EGC 49.248 ± 2.943a 39.766 ± 3.377b 32.811 ± 4.212c 17.511 ± 3.132d 16.029 ± 8.675d 11.673 ± 6.057e 4.117 ± 3.220f 1.577 ± 1.380f 2.800 ± 2.116f 3.122 ± 1.482f C 34.730 ± 12.072a 25.859 ± 4.542b 25.784 ± 4.899b 21.133 ± 3.301c 20.225 ± 1.656c 17.996 ± 1.792ce 14.164 ± 0.725ef 10.011 ± 1.040f 11.188 ± 2.066f 10.487 ± 1.299f EC 24.382 ± 5.110a 22.508 ± 3.291ab 21.541 ± 2.957b 18.028 ± 2.620c 15.915 ± 0.365cd 13.624 ± 0.336d 9.521 ± 0.851e 6.231 ± 1.347f 7.345 ± 0.583ef 6.689 ± 0.306f EGCG 250.087 ± 3.465a 230.995 ± 7.142ab 214..127 ± 12.789b 166.138 ± 12.478c 143.142 ± 37.211d 113.768 ± 33.531e 55.881 ± 29.028f 21.082 ± 14.399g 29.749 ± 14.769g 33.393 ± 7.166g GCG 0.901 ± 0.082a 0.928 ± 0.082a 0.858 ± 0.096a 0.658 ± 0.059b 0.581 ± 0.160b 0.407 ± 0.131c 0.211 ± 0.084d 0.081 ± 0.037e 0.135 ± 0.055de 0.214 ± 0.0254d ECG 37.392 ± 7.412a 34.177 ± 4.864b 33.653 ± 3.649b 31.376 ± 3.937bc 28.058 ± 1.001cd 25.238 ± 1.621d 20.064 ± 0.703e 14.346 ± 0.896f 14.381 ± 1.357f 13.458 ± 0.861f Total catechins 405.470 ± 30.134a 361.106 ± 15.296b 335.169 ± 13.269b 258.990 ± 17.723c 227.780 ± 44.836e 185.647 ± 37.863f 105.401 ± 33.025e 53.940 ± 17.880g 66.526 ± 14.339g 68.489 ± 10.870g GA 0.680 ± 0.108f 1.440 ± 0.277e 1.988 ± 0.254e 3.475 ± 0.271d 3.584 ± 0.707d 4.027 ± .0.730d 5.398 ± 0.437c 5.821 ± 0.218c 6.755 ± 0.891b 8.187 ± 1.247a Caffeine 68.601 ± 4.509ac 71.635 ± 6.686abc 71.447 ± 4.596abc 73.500 ± 5.649c 71.140 ± 4.084abc 71.147 ± 5.983abc 70.789 ± 5.863abc 73.469 ± 6.177ce 74.056 ± 4.884bc 67.746 ± 2.673a Vitexin-
2-O-rhamnoside0.205 ± 0.077bc 0.338 ± 0.205b 0.347 ± 0.210b 0.354 ± 0.205b 0.336 ± 0.198b 0.320 ± 0.190b 0.317 ± 0.196b 0.325 ± 0.212b 0.360 ± 0.320b 0.418 ± 0.247ab Quercetin-
3-O-rutinoside1.873 ± 0.339ab 1.776 ± 0.427b 1.746 ± 0.418abc 1.783 ± 0.426ab 1.688 ± 0.509abc 1.524 ± 0.419abc 1.508 ± 0.349abc 1.366 ± 0.246c 1.417 ± 0.367bc 1.362 ± 0.345c Quercetin-
3-O-galactoside0.853 ± 0.684a 0.808 ± 0.659a 0.798 ± 0.656a 0.808 ± 0.661a 0.784 ± 0.636a 0.743 ± 0.582a 0.722 ± 0.514a 0.709 ± 0.485a 0.735 ± 0.508a 0.696 ± 0.472a Cynaroside 0.046 ± 0.007acd 0.052 ± 0.011cd 0.050 ± 0.013acd 0.052 ± 0.010acd 0.048 ± 0.012acde 0.044 ± 0.009acd 0.040 ± 0.009abe 0.036 ± 0.007b 0.034 ± 0.006b 0.034 ± 0.006b Quercetin
3-O-rhamnoside0.022 ± 0.010acd 0.022 ± 0.006acd 0.021 ± 0.005cde 0.025 ± 0.008d 0.019 ± 0.004abce 0.019 ± 0.003abce 0.016 ± 0.002e 0.016 ± 0.001be 0.015 ± 0.003be 0.015 ± 0.002be Luteolin 0.001 ± 0.000ac 0.001 ± 0.000ac 0.001 ± 0.001ac 0.002 ± 0.001cde 0.002 ± 0.001de 0.002 ± 0.001e 0.004 ± 0.001f 0.006 ± 0.001h 0.005 ± 0.001g 0.009 ± 0.001b Quercetin 0.053 ± 0.029a 0.047 ± 0.027a 0.052 ± 0.032a 0.051 ± 0.031a 0.050 ± 0.029a 0.048 ± 0.027a 0.044 ± 0.024a 0.045 ± 0.025a 0.069 ± 0.048a 0.068 ± 0.041a Kaempferol 0.046 ± 0.010a 0.042 ± 0.014a 0.046 ± 0.018a 0.047 ± 0.019a 0.045 ± 0.016a 0.43 ± .015a 0.045 ± 0.016a 0.050 ± 0.021a 0.066 ± 0.035a 0.077 ± 0.040b Vitexin 0.037 ± 0.008a 0.043 ± 0.006bcd 0.046 ± 0.006cd 0.046 ± 0.008d 0.043 ± 0.004bcd 0.041 ± 0.006abcd 0.040 ± 0.006ab 0.039 ± 0.006ab 0.041 ± 0.005abcd 0.046 ± 0.004bcd Different lowercase letters following the number indicate significant differences during the processing (p < 0.05). The results were presented in the form of mean values followed by the standard deviation. Table 1.
Dynamic variation of major taste compounds.
Figures
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Tables
(1)