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Figure 1. 

Characterization of the fluorescent glucosamine derivative. (a) Isolated compound 1a and 1b mixture after silica-gel chromatography purification. (b) TLC analysis by irradiation with UV light (left: 362 nm, right: 254 nm). (c) HPLC chromatogram of compounds 1a and 1b (excitation 362 nm, emission 450 nm). (d) Positive ion electrospray-ionization (ESI) mass spectrum of compounds 1a and 1b.

Figure 2. 

Reaction of GlcN with DPPD to afford fluorescent glucosamine derivatives 1a and 1b with atom numbering corresponding to that used for NMR assignment.

Figure 3. 

Characterization of GPDA wild-type and mutant variants. (a) Precipitation behavior of wild-type GPDA in the presence of various concentrations of GlcN. (b) Protein sequence comparison of the selected GPDA mutant variants. (c) Comparison of protein yields of GPDA mutant and wild-type variants. (d) Precipitation behavior of the GPDA variants in the presence of 50 mM GlcN.

Figure 4. 

(a) Protein precipitation behavior of GPDA mutant variants and (b) visualization of the GlcN binding site of the GPDA wild-type and mutant variants protein models generated by AlphaFold.