Back Article

Figure 1. 

Phylogenetic relationships, gene structure and conserved protein motifs of CYP78A genes from eggplant, Arabidopsis, rice and tomato. (a) The phylogenetic tree was constructed based on the full-length protein sequences of six AtCYP78As, nine OsCYP78As, six SlCYP78As and six SmCYP78As proteins using MEGA 7.0 software. Eggplant, Arabidopsis, rice and tomato CYP78As were labeled by red, black, pink and green dots. (b) Exon-intron structure of CYP78A genes. Black lines indicate introns. The number indicates the phases of corresponding introns. (c) The motif composition of CYP78A proteins. The motifs, numbers 1–10, are displayed in different colored boxes. The sequence logos and E values for each motif are given in Supplemental Fig. S1.

Figure 2. 

Gene duplication and synteny analysis of SmCYP78A genes. (a) Schematic representations for the chromosomal distribution and interchromosomal relationships of SmCYP78A genes. Gray lines indicate all synteny blocks in the eggplant genome, and the red lines indicate segmental duplicated SmCYP78A gene pairs. (b) Synteny analysis of CYP78A genes between eggplant and Arabidopsis. (c) Synteny analysis of CYP78A genes between eggplant and tomato. (d) Synteny analysis of CYP78A genes between eggplant and rice. Gray lines in the background indicate the collinear blocks between genomes, while the red lines highlight the syntenic blocks harboring CYP78A gene pairs.

Figure 3. 

Expression profiles of the six SmCYP78A genes in different tissues. (a) Relative transcript abundances of the SmCYP78A genes examined by qRT-PCR. (b) Expression of the SmCYP78A genes in young flower buds and developing ovaries detected by RNA-seq. Rt, Root; St, Stems; Le, Leaf; Pet, Petal; Se, Sepal; Per, Pericarp; FF, Fruit flesh; YB, Young flower buds; DBA, Days before anthesis; DPA, Days post anthesis.

Figure 4. 

Twelve co-expressed clusters are clustered using fuzzy C-means clustering in Mfuzz with normalized expression values (z-scores). The red lines represent the average of expression values, whereas the gray lines represent the expression values of the co-expressed genes. YB, Young flower buds; DBA, Days before anthesis; DPA, Days post anthesis.

Figure 5. 

Significantly enriched GO terms (biological process) of co-expression genes in (a) Cluster 6 and (b) Cluster 11. Only the top five enriched GO terms are shown. The color of lines represents different GO terms.

Figure 6. 

Overview of distribution of TF families that were co-expressed with (a) SmCYP78A10 in Cluster 6 and (b) SmCYP78A5 in Cluster 11. The Plant Transcription Factor Database v5.0 (http://planttfdb.gao-lab.org) was used to identify TFs in the eggplant genome.