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Figure 1.
Schematic diagram of artificial pollination.
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Figure 2.
Comparison of fruit-setting ratios among hybrid combinations.
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Figure 3.
Comparison of fruit-setting ratio in 2010 and 2011.
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Figure 4.
Rainfall in 2010 and 2011.
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Figure 5.
Number of mismatching bands in the false-hybrid individuals.
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Figure 6.
Influence of the number of primers for false-hybrid identification (95% confidence).
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Code Hybrid combination Quantitya L × B Longjing 43♀ × Baihaozao♂ 1250 J × L Jiaming 1♀ × Longjing 43♂ 1350 B × L Baihaozao♀ × Longjing 43♂ 1200 a Quantity of pollinated flowers. Table 1.
Experimental artificial hybrid combinations.
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Name Forward primer Reverse primer NCBI accession LGa Nab PICc A27 TTGCTGGGGTTGTCTATG TCAATTGTGGCCAATTCT JQ398741 LG07 2 0.294 A41 GATTCTGCTGCCATTGTATTCC CGCCTCGTCTTCGTCGTTA EU787433 LG05 3 0.369 A44 TGCAACTCGGCCTTCTGT GGAGCTGACTTAGCCCTCA FE861192 LG11 4 0.7 A114 TAGCTTTGTGGAGCCTGAG ATCTTCCTTCGCTAACCC CV013769 LG11 2 0.316 A134 ACCCAAAATATGAAACAACATG TGACTACTGCACTGACACTGCTA − LG09 2 0.297 A142 ATGCTTCAGGGAGTGACCAT ATTTATGCCAAACTACCAACAG FE861636 LG02 4 0.547 A157 GGGGTTAGGGTTTTGGTCAC ATGCCTTAACAAGAACACTACAAG FE942901 LG11 2 0.275 A187 CCTCCTGCTTTGTTTATGCT TACCGACAATCACTTGTGGATC FE861247 LG12 2 0.32 CsFM1012 AGCCACCATACCCACACATT ACAAACCAATGCTACTGCCC GAAC01011288 LG01 3 0.541 CsFM1051 AACCCATTTCGTCTTTGTGC AGAATCAACAACACCCTGGC GAAC01009709 LG08 2 0.316 CsFM1061 TTGGCAGGAAAAGAAAAGGA AGCAGCGAGTTCTTTGTGGT GAAC01002340 LG03 3 0.543 CsFM1068 CAGGCCTTCGTTTTCTTTTG TTCCTCATCTTCTGTCTCCCTC GAAC01009576 LG15 3 0.55 CsFM1097 CGGCAGATTTGGTGATAGGA AACAAAATGGGAACCCACAA GAAC01004794 LG09 4 0.563 CsFM1102 GCATACACTCTTCCGGTGGT ACAATCTCAACCTCCATCCG GAAC01008204 LG11 3 0.318 CsFM1156 CACAAGGAGGGGGTACGTTA CACCTTCCACAGAGGGAGAC GAAC01009838 LG02 3 0.567 CsFM1158 CGTGCCTGCATTGCTAATAA GTACCAGTAACTGCGGGGAA fd_17402 LG12 3 0.295 CsFM1298 TTGTTTGATTTGCCGGTGTA TTGAAGGATCTCCGAAGGAA GAAC01015282 LG08 3 0.309 CsFM1349 TGGGCCAGAAGAGAAAAGAA GGTGTTCCTGGCACTTCAAT GAAC01012393 LG12 3 0.543 CsFM1357 TCGGCGATGGAAAAGATAAC CTTGTGAACGGGCTTCTCTC GAAC01009805 LG13 3 0.567 CsFM1390 TCTCCCTCTCTCCTCTGCAC GGAGCAACCCAAAACTGCTA GAAC01008822 LG01 3 0.285 CsFM1411 ATACTCTGCTCCGTCTGCGT TCGGTGCTACAAACAAATGC GAAC01010715 LG01 4 0.395 CsFM1435 CCCGTGACACAATTCACATC CTGGGCATATGCTGGTGTTA GAAC01011748 LG03 3 0.302 CsFM1459 TTTTTCACGGTAAAGTCGGG CCCTAGCTGCAGAAACCATC GAAC01013904 LG08 3 0.558 CsFM1504 TGGTAAACGGAAAGAATGGC AGCTAGCCCTGTGAAAACCA GAAC01005613 LG03 2 0.298 CsFM1509 GACGATGGACCCTTCTTTGA CATCATCATCATCCTCACCG GAAC01005346 LG14 3 0.561 CsFM1529 TGTTCTTGATTTCCTCTACTCCA TTTGAGGGAAAGGTGAGGAG GAAC01049476 LG11 3 0.379 CsFM1550 CGAGACATCGAACACCACAG CGTATCGTAGCGGTGAAGGT GAAC01040688 LG13 3 0.551 CsFM1562 CTTCCAGAAGTCCCATCGAA CTCTCTCCCAAAACGTCCAG GAAC01038212 LG09 3 0.37 CsFM1565 CAAATTTTGTCGTCGTCGTG TCGCCTAAATTCGTGAGCTT GAAC01003369 LG03 2 0.307 CsFM1597 GGTGGTGATCGTCTTGGTCT AAACGAAACGAAACGAATGG GAAC01002473 LG08 4 0.566 CsSSR103 TGGCAGTCATGATAGGCATT TGCTGCAAATGATGAACACA HP770422 LG03 3 0.339 CsSSR117 CCACCGTCGTCTTCTTCTTC CTCGTTGTTGGAGTTGCTGA KA283538 LG10 3 0.53 a Linkage group (LG). b Number of allele (Na) in J × L. c Polymorphism information content (PIC) in J × L. Table 2.
SSR marker information.
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Code name PEa PUb SQc GR F1 (%)d SR F1 (%)e L × B 500−600 5 537 70.90 96.32 J × L 500−600 5
299
78.26
98.29
B × L
500−600
5
220
45.45
75.00
Mean352 65.87 89.87 a Pollination efficiency. The quantity of per capita pollination per day (PE). b Pollen utilization. The quantity of pistillate flowers pollinated by one staminate flower (PU). c Seed quantity (SQ). d Germination rate of F1 group (GR). e Survival rate of F1 group (SR). Table 3.
Pollination efficiency and growth of F1 progeny.
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Code Confidence Critical Δ No. of
false-hybridFalse-hybrid
rate (%)J × L 99% 3.75 16 8.70 95% > 0 9 4.89 Table 4.
Parentage identification statistics.
Figures
(6)
Tables
(4)