Figures (6)  Tables (4)
    • Figure 1. 

      Schematic diagram of artificial pollination.

    • Figure 2. 

      Comparison of fruit-setting ratios among hybrid combinations.

    • Figure 3. 

      Comparison of fruit-setting ratio in 2010 and 2011.

    • Figure 4. 

      Rainfall in 2010 and 2011.

    • Figure 5. 

      Number of mismatching bands in the false-hybrid individuals.

    • Figure 6. 

      Influence of the number of primers for false-hybrid identification (95% confidence).

    • CodeHybrid combinationQuantitya
      L × BLongjing 43♀ × Baihaozao♂1250
      J × LJiaming 1♀ × Longjing 43♂1350
      B × LBaihaozao♀ × Longjing 43♂1200
      a Quantity of pollinated flowers.

      Table 1. 

      Experimental artificial hybrid combinations.

    • NameForward primerReverse primerNCBI accessionLGaNabPICc
      A27TTGCTGGGGTTGTCTATGTCAATTGTGGCCAATTCTJQ398741LG0720.294
      A41GATTCTGCTGCCATTGTATTCCCGCCTCGTCTTCGTCGTTAEU787433LG0530.369
      A44TGCAACTCGGCCTTCTGTGGAGCTGACTTAGCCCTCAFE861192LG1140.7
      A114TAGCTTTGTGGAGCCTGAGATCTTCCTTCGCTAACCCCV013769LG1120.316
      A134ACCCAAAATATGAAACAACATGTGACTACTGCACTGACACTGCTALG0920.297
      A142ATGCTTCAGGGAGTGACCATATTTATGCCAAACTACCAACAGFE861636LG0240.547
      A157GGGGTTAGGGTTTTGGTCACATGCCTTAACAAGAACACTACAAGFE942901LG1120.275
      A187CCTCCTGCTTTGTTTATGCTTACCGACAATCACTTGTGGATCFE861247LG1220.32
      CsFM1012AGCCACCATACCCACACATTACAAACCAATGCTACTGCCCGAAC01011288LG0130.541
      CsFM1051AACCCATTTCGTCTTTGTGCAGAATCAACAACACCCTGGCGAAC01009709LG0820.316
      CsFM1061TTGGCAGGAAAAGAAAAGGAAGCAGCGAGTTCTTTGTGGTGAAC01002340LG0330.543
      CsFM1068CAGGCCTTCGTTTTCTTTTGTTCCTCATCTTCTGTCTCCCTCGAAC01009576LG1530.55
      CsFM1097CGGCAGATTTGGTGATAGGAAACAAAATGGGAACCCACAAGAAC01004794LG0940.563
      CsFM1102GCATACACTCTTCCGGTGGTACAATCTCAACCTCCATCCGGAAC01008204LG1130.318
      CsFM1156CACAAGGAGGGGGTACGTTACACCTTCCACAGAGGGAGACGAAC01009838LG0230.567
      CsFM1158CGTGCCTGCATTGCTAATAAGTACCAGTAACTGCGGGGAAfd_17402LG1230.295
      CsFM1298TTGTTTGATTTGCCGGTGTATTGAAGGATCTCCGAAGGAAGAAC01015282LG0830.309
      CsFM1349TGGGCCAGAAGAGAAAAGAAGGTGTTCCTGGCACTTCAATGAAC01012393LG1230.543
      CsFM1357TCGGCGATGGAAAAGATAACCTTGTGAACGGGCTTCTCTCGAAC01009805LG1330.567
      CsFM1390TCTCCCTCTCTCCTCTGCACGGAGCAACCCAAAACTGCTAGAAC01008822LG0130.285
      CsFM1411ATACTCTGCTCCGTCTGCGTTCGGTGCTACAAACAAATGCGAAC01010715LG0140.395
      CsFM1435CCCGTGACACAATTCACATCCTGGGCATATGCTGGTGTTAGAAC01011748LG0330.302
      CsFM1459TTTTTCACGGTAAAGTCGGGCCCTAGCTGCAGAAACCATCGAAC01013904LG0830.558
      CsFM1504TGGTAAACGGAAAGAATGGCAGCTAGCCCTGTGAAAACCAGAAC01005613LG0320.298
      CsFM1509GACGATGGACCCTTCTTTGACATCATCATCATCCTCACCGGAAC01005346LG1430.561
      CsFM1529TGTTCTTGATTTCCTCTACTCCATTTGAGGGAAAGGTGAGGAGGAAC01049476LG1130.379
      CsFM1550CGAGACATCGAACACCACAGCGTATCGTAGCGGTGAAGGTGAAC01040688LG1330.551
      CsFM1562CTTCCAGAAGTCCCATCGAACTCTCTCCCAAAACGTCCAGGAAC01038212LG0930.37
      CsFM1565CAAATTTTGTCGTCGTCGTGTCGCCTAAATTCGTGAGCTTGAAC01003369LG0320.307
      CsFM1597GGTGGTGATCGTCTTGGTCTAAACGAAACGAAACGAATGGGAAC01002473LG0840.566
      CsSSR103TGGCAGTCATGATAGGCATTTGCTGCAAATGATGAACACAHP770422LG0330.339
      CsSSR117CCACCGTCGTCTTCTTCTTCCTCGTTGTTGGAGTTGCTGAKA283538LG1030.53
      a Linkage group (LG). b Number of allele (Na) in J × L. c Polymorphism information content (PIC) in J × L.

      Table 2. 

      SSR marker information.

    • Code namePEaPUbSQcGR F1 (%)dSR F1 (%)e
      L × B500−600553770.9096.32
      J × L500−6005
      299

      78.26

      98.29

      B × L

      500−600

      5

      220

      45.45

      75.00

      Mean
      35265.8789.87
      a Pollination efficiency. The quantity of per capita pollination per day (PE). b Pollen utilization. The quantity of pistillate flowers pollinated by one staminate flower (PU). c Seed quantity (SQ). d Germination rate of F1 group (GR). e Survival rate of F1 group (SR).

      Table 3. 

      Pollination efficiency and growth of F1 progeny.

    • CodeConfidenceCritical ΔNo. of
      false-hybrid
      False-hybrid
      rate (%)
      J × L99%3.75168.70
      95%> 094.89

      Table 4. 

      Parentage identification statistics.