Effects of different pretreatments on the physiochemical properties of pig manure and its potential in promoting the growth and nutrient accumulation of black soldier fly larvae (BSFL)

Xiaofei Wu; Wenyu Zhao; Xuan Wang; Zhaohai Bai; Lin Ma; Xiaofei Wu; Wenyu Zhao; Xuan Wang; Zhaohai Bai; Lin Ma

doi:10.48130/CAS-2023-0003

Figures (5) Tables (1)

Figure 1.
Changes in physicochemical properties of pig manure after different pretreatments. (a) pH and EC; (b) TC and TN; (c) DOC and DON; (d) NH4⁺-N. CK, Control; AE-2, Aerobic fermentation for 2 d; AN-2, Anaerobic fermentation for 2 d; HAN-2, High temperature anaerobic fermentation for 2 d; AE-5, Aerobic fermentation for 5 d; AN-5, Anaerobic fermentation for 5 d; HAN-5, High temperature anaerobic fermentation for 5 d.
Figure 2.
Changes in protein and amino acids of pig manure after different pretreatments. (a) Protein content; (b) amino acid content. CK, Control; AE-2, Aerobic fermentation for 2 d; AN-2, Anaerobic fermentation for 2 d; HAN-2, High temperature anaerobic fermentation for 2 d; AE-5, Aerobic fermentation for 5 d; AN-5, Anaerobic fermentation for 5 d; HAN-5, High temperature anaerobic fermentation for 5 d.
Figure 3.
Growth and nutritional characteristics of BSFL fed with pig manure with different pretreatments. (a) Weight, (b) protein content, (c) fat content, (d) amino acid content. CK, Control; AE-2, Aerobic fermentation for 2 d; AN-2, Anaerobic fermentation for 2 d; HAN-2, High temperature anaerobic fermentation for 2 d; AE-5, Aerobic fermentation for 5 d; AN-5, Anaerobic fermentation for 5 d; HAN-5, High temperature anaerobic fermentation for 5 d.
Figure 4.
(a) PCoA analysis of physicochemical properties of different pretreated pig manure and (b) RDA analysis of black soldier fly fed on different pretreated pig manure.
Figure 5.
Correlation analysis between performance of (a) BSFL and physicochemical properties of pig manure and (b) RDA analysis. SR, Survival rate; WR, Waste reduction rate; BR, Bioconversion rate; WCE, Waste conversion efficiency; PCE, Protein conversion efficiency.

Different treatments	Survival rate (%)	Waste reduction (%)	Bioconversion rate (%)	Protein conversion efficiency (%)
CK	95.67 ± 0.57^bcd	26.33 ± 3.01^ab	4.94 ± 1.84^bc	7.59 ± 0.011^e
AE-2	94.33 ± 1.52^cd	22.07 ± 6.80^ab	4.34 ± 1.07^bc	6.41 ± 0.036^f
AN-2	96.00 ± 1.00^bc	27.63 ± 8.25^ab	5.20 ± 1.64^abc	8.08 ± 0.061^c
HAN-2	98.67 ± 0.57^a	29.74 ± 7.95^a	7.32 ± 0.73^a	8.91 ± 0.023^a
AE-5	94.00 ± 1.00^d	19.52 ± 2.85^b	3.81 ± 1.63^c	5.09 ± 0.044^g
AN-5	95.67 ± 0.58^bcd	28.48 ± 4.54^ab	6.49 ± 1.64^ab	7.82 ± 0.017^d
HAN-5	97.33 ± 0.58^ab	28.97 ± 2.95^a	7.01 ± 1.08^ab	8.70 ± 0.032^b
The values before and after ± are mean values and standard deviation, respectively. Different lowercase letters indicate a significant difference (p < 0.05).

Table 1.

Performance of BSFL fed on different pretreated manure.