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Figure 1. 

Phylogenetic relationships of PbWOX proteins. (a) NJ tree constructed of the amino acid sequence of WOXs from Phoebe bournei (Pb), Arabidopsis thaliana (At), Populus trichocarpa (Pt), Oryza sativa (Os), Amborella trichopoda (Atri), Selaginella moellendorffii (Sm), Physcomitrella patens (Ppa), Ostreococcus tauri (Ostau) and Ostreococcus lucimarinus (Ostlu). (b) Synteny analysis of WOX genes between P. bournei and A. thaliana. Gray lines indicate all synteny blocks in the genome, and the red lines indicate duplicated WOX gene pairs.

Figure 2. 

Information on the PbWOX genes and proteins. (a) Phylogenetic tree and gene structure. (b) Architecture of conserved protein motifs. (c) Multiple sequence alignment.

Figure 3. 

Predicted cis-acting elements in PbWOX promoters. (a) Frequency of cis-acting elements in the 2.0 kb upstream regions of PbWOXs. The corresponding colored bar chart indicates the occurrence of different cis-acting elements. (b) Number of cis-acting elements in each WOX gene.

Figure 4. 

Semiquantitative analysis of PbWOXs in different tissues. (a) Tissue samples, 1 - epicotyl, 2 - stem tip, 3 - root, 4 - stem, 5 - leaf, 6 - calli. (b) Semiquantitative PCR electropherogram.

Figure 5. 

Expression patterns of PbWOXs during SE of P. bournei. (a) Calli-1. (b) Calli-2. (c) Calli-3. (d) Globular embryo. (e) Immature cotyledon-producing embryo. (f) Mature cotyledon-producing embryo. (g) Analysis of gene expression via qPCR. The data are the means ± SDs of three biological replicates. The values followed by the same letter are not different according to Duncan’s multiple-range test. PbEF1α was used as an endogenous control.

Figure 6. 

Relative expression levels of PbWOXs under hormone treatment. The data are the means ± SDs of three biological replicates. The values followed by the same letter are not different according to Duncan's multiple-range test. PbEF1α was used as an endogenous control.