Effects of TDZ and IBA on DNA methylation during callus induction in pear (<i>Pyrus ussuriensis</i> Maxim)

Figures (3) Tables (3)

Figure 1.
Comparison of pear callus induced by different TDZ and IBA concentrations. Scale bars = 0.5 cm.
Figure 2.
Expression analysis of methylation related genes in pear callus induced by different TDZ and IBA concentrations.
Figure 3.
Analysis of expression of auxin and cytokinin-related genes in pear callus induced by different TDZ and IBA concentrations.

PGR Combination		No. of total explants	No. of callus	Induction rate (%)
2.0 mg·L⁻¹ IBA	3.0 mg·L⁻¹ TDZ	50	13	24.00 ± 3.06^b
		50	14
		50	9
4.0 mg·L⁻¹ IBA		50	26	54.00 ± 2.00^a
		50	26
		50	29
6.0 mg·L⁻¹ IBA		50	10	11.33 ± 4.67^cd
		50	5
		50	2
1.0 mg·L⁻¹ TDZ	4.0 mg·L⁻¹ IBA	50	7	14.67 ± 2.91^c
		50	5
		50	10
3.0 mg·L⁻¹ TDZ		50	26	54.00 ± 2.00^a
		50	26
		50	29
5.0 mg·L⁻¹ TDZ		50	2	4.67 ± 1.76^d
		50	4
		50	1
Samples were collected 2 weeks after induction in the dark. Only callus with sizes greater than or equal to 2 mm × 2 mm × 2 mm were counted. All analysis were repeated three times and the mean ± S.E. was calculated. Callus induction rate = number of callus in existence/total number of explants × 100%. Columns with different letters are significantly difference at P < 0.05 by Duncan’s multiple range test.

Table 1.

Effects of TDZ and IBA on the differentiation of pear leaf explants.

Table 2.

Comparison of DNA methylation level in pear callus induced by different TDZ and IBA concentrations.

Table 3.

MSAP pattern of pear callus induced by different TDZ and IBA concentrations.