Screening and identification of a lignocellulose-degrading strain and its application in highland barley fermentation

Figures (6) Tables (2)

Figure 1.
(a) Aniline blue fading results and (b) decolorization rate of different strains.
Figure 2.
(a) Ligninase activity and (b) cellulase activity of different strains.
Figure 3.
Colony, cell morphologies and spore of the isolated ligninase and cellulase-producing strain LM1. (a) Colony morphology 12 h, (b) colony morphology 24 h, (c) cell morphology, (d) spore.
Figure 4.
Phylogenetic trees based on (a) 16S rRNA gene sequence and (b) gyrA gene sequence of strain LM1.
Figure 5.
Hemolysis test of (a) B. velezensis LM1 and (b) B. cereus ATCC14579.
Figure 6.
(a) Growth curve and the enzyme activities of (b) CMCase and FPA, (c) Lac, Lip and Mnp, (d) amylase and protease of B. velezensis LM1.

Strains	Diameter of colony (D/mm)	Diameter of transparent circle (d/mm)	d/D	Degree of disintegration
LX1	4.23 ± 0.01^b	14.62 ± 0.21^d	3.46	++
LX4	4.61 ± 0.18^a	23.79 ± 0.64^b	5.16	++++
LX12	3.55 ± 0.05^c	18.07 ± 0.09^c	5.10	+++
LM1	4.77 ± 0.01^a	27.02 ± 0.02^a	5.66	+++++
LM5	4.29 ± 0.04^b	9.25 ± 0.52^e	2.16	+
LM6	4.84 ± 0.18^a	27.83 ± 1.39^a	5.76	++++
Lowercase letters represent significant differences in the same column. '+' represents the degree of damage to the filter paper, and the more '+', the stronger the degree of damage.

Table 1.

Congo red transparent circle and filter paper strip disintegration effects of different strains.

Sample	Yield (%)	Purity (%)
RSDF	6.07 ± 0.01^B	87.00 ± 0.30^A
FSDF	12.57 ± 0.22^A	87.13 ± 0.99^A
RIDF	22.45 ± 0.02^a	91.70 ± 0.82^a
FIDF	18.68 ± 0.21^b	77.27 ± 0.50^b
^{A, B} reflect the significance of SDF, and ^{a, b} reflect the significance of IDF. P < 0.05.

Table 2.

The yield and purity of dietary fiber.