Figures (2)  Tables (2)

    • Figure 1. 

      Phylogram generated from maximum likelihood analysis based on combined dataset of ITS, LSU, SSU and RPB2 sequence data of Microascales group. Bootstrap support values for maximum likelihood (ML) equal to or greater than 75% and Bayesian posterior probabilities (PP) equal to or greater than 0.90 are given above the nodes. A newly generated sequence and a newly introduced family are in blue bold. Type species are in bold. The tree is rooted with Falcocladium sphaeropedunculatum (CBS 111292) and F. multivesiculatum (CBS 120386).

    • Figure 2. 

      Triadelphia mukdahanensis (holotype). (a)–(d) Appearance of colonies on substrate. (e)–(g) Conidiogenous cells and conidia (e and f mounted in 5% KOH). (f)–(k) conidial type (a). (l)–(n) Conidial type (b). (o)–(p) Conidial type (f). (q) Conidial type (a), (b) and (f). Scale bars: a–c = 500 µm, d = 20 µm, e–i, k–n, q = 10 µm. j, o, p = 5 µm.

    • TaxaStrain no.1GenBank accession numbers2
      ITSLSUSSURPB2
      Acaulium albonigrescensCBS 109.69KY852469KY852480−−
      Ambrosiella xyleboriCBS 110.61−EU984294AY858659−
      Ceratocystis fagacearumCMW 2656−KM495341−−
      Corollospora marinaAFTOL-ID 5008−AY762985FJ176843−
      Corollospora ramulosaPP1232−AF491276−−
      Falcocladium multivesiculatumCBS 120386TEU040217JF831932JF831928−
      Falcocladium sphaeropedunculatumCBS 111292TJF831938JF831933JF831929−
      Faurelina elongataCBS 126.78GU291802DQ368625DQ368657DQ368639
      Faurelina fimigenaCBS 352.78TMH861149−−−
      Graphium fimbriasporumCMW 5605TNR_155108NG_067529NG_064882−
      Graphium fimbriisporumCMW 5606AY148180−AY148172−
      Graphium laricisCMW 5601TAY148183NG_064370AY148162−
      Graphium penicillioidesCBS 102632TKY852474NG_067324−−
      Graphium pseudormiticumCMW 503TNR_136962NG_064371NG_064881−
      Knoxdaviesia capensisAFTOL-ID 1907−FJ176888FJ176834−
      Knoxdaviesia proteaeCBS 486.88TAY372072AF221011AY271804−
      Knoxdaviesia serotectaCBS 129738TMH865536MH876970−−
      Knoxdaviesia ubusiCBS 129742TMH865540KM495395−−
      Knoxdaviesia wingfieldiiCBS 132470TNR_111723NG_042656−−
      Microascus longirostrisAFTOL-ID 1237−−DQ471026−
      Nimbospora effusaJKI 5104A−U46892U46877DQ836887
      Parascedosporium tectonaeCBS 127.84TMH861707EF151332U43907−
      Pseudoscopulariopsis schumacheriCBS 435.86TLM652455AF400874−−
      Synnematotriadelphia stilboideaCBS 101294MF434783MF434793MF434811MF434802
      Synnematotriadelphia stilboideaCBS 221.85TMF434781MF434792MF434810MF434801
      Triadelphia disseminataCBS 138592TMF434784MF434788MF434806MF434797
      Triadelphia diversaCBS 113.90TMF434782MF434790MF434808MF434799
      Triadelphia fusiformisMFLUCC 16-0231TMH777097MH777098−−
      Triadelphia heterosporaCBS 222.83TMF434779MF434789MF434807MF434798
      Triadelphia hexaformisporaTBRC 9288TMK588842MK588850MK588840MK578528
      Triadelphia loudetiaeCBS 589.77TMF434776MF434785MF434803MF434794
      Triadelphia moubasheriAUMC 10746TKY611849−−−
      Triadelphia mukdahanensisMFLUCC 23-0049 TOQ871494OQ871495OQ883648OQ871561
      Triadelphia pulvinataCBS 590.77TMF434777MF434786MF434804MF434795
      Triadelphia pulvinataCBS 744.84MF434780MF434787MF434805MF434796
      Triadelphia romanicaCBS 162.79TMF434778MF434791MF434809MF434800
      Wardomyces anomalusCBS 299.61LN850992LN851044−−
      1 AFTOL-ID: Assembling the Fungal Tree of Life; AUMC: Assiut University Mycological Centre; CBS: Westerdijk Fungal Biodiversity Institute, Utrecht, The Netherlands; CMW: Collection of the Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, South Africa; JKI: Culture collection of the Julius Kühn-Institute, Siebeldingen, Germany; MFLUCC: Mae Fah Luang University Culture Collection, Chiang Rai, Thailand; TBRC: Thailand Bioresource Research Center; T: ex-type isolates. 2 ITS: internal transcribed spacer regions 1 and 2 including 5.8S nrRNA gene; LSU: 28S large subunit of the nrRNA gene; SSU: 18S small subunit of the nrRNA gene; RPB2: partial RNA polymerase II second largest subunit gene.

      Table 1. 

      Taxa used in this study and their GenBank accession numbers. New sequences are in bold.

    • Conidial formsT. mukdahanensis
      (this study)      		T. heterospora
      (Shearer & Crane[23])      		T. heterospora
      (Constantinescu & Samson[24])
      Type (a):
      oblong to cylindrical, brown to dark brown, (1–)2–septate, septum covered with dark band      		(9.5–)18–25 ×
      (3.5–)5–7(–8.5) µm      		(14.7–)16.2–19(–21.2) ×
      3.5–6 µm      		15–23 × 5–8.5 µm
      Type (b):
      pyriform to broadly clavate, or club-shaped, brown to dark brown, (1–)2–septate, the apical septum covered with broad dark bands, the basal cell subhyaline or light brown to brown      		20–30 × 7–12 µm––
      Type (c):
      obclavate to acicular with a narrow long tip, hyaline or yellowish brown, multiseptate      		–––
      Type (d):
      fusiform, obclavate with acicular tips, or rounded at the tip, multi-septate, end cells pale brown, central cells brown to dark brown, dark band covering over the central septa      		–17.6–23.5 × 8.2–10.6 µm17.6–23.5 × 8.2–10.6 µm
      Type (e):
      allantoid or reniform, hyaline or pale yellowish, 0–3-septate
      		––8.5–15 × 3–5 µm
      Type (f):
      obovate to broadly ellipsoidal, pale brown, unicellular      		5–9 × 3.5–5 µm–6–8 × 4–5 µm

      Table 2. 

      Conidial forms comparison of Triadelphia mukdahanensis and T. heterospora discussed in this study.