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Figure 1.
Important historical milestones and future perspectives in gene transformation strategies and their application of Carica papaya L.
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Trait improvement Gene introduced Transformation method Reference Virus resistance PRSV resistance Translatable/ Untranslatable coat protein (CP) Biolistics [15,16,39−42,44,45] Translatable/ Untranslatable CP Agrobacterium [21,24,25,34,39,
41−43, 46−48]Replicase Agrobacterium [34] Untranslatable CP Pollen tube [30] PRSV and PLDMV resistance Untranslatable CP Agrobacterium [26,52] Fungal resistance Phytophthora palmivora resistance Stilbene synthase Vst1 Biolistics [53] Antimicrobial peptide DmAMP1 Biolistics [54] NPR1 Biolistics [55] Bacterial resistance Dieback disease resistance Acyl-homoserine lactonase Agrobacterium [61] Insect resistance Carmine spider mite resistance Chitinase Biolistics [62] Lectin GNA Biolistics [66] Abiotic stresses tolerance Herbicide resistance Bar Biolistics [17] Aluminum tolerance Citrate synthase Biolistics [68] Improvement of shelf life Untranslatable ACC oxidase (ACO) Biolistics [72] Untranslatable ACO1 and ACO2 Agrobacterium [60,73] Untranslatable ACC synthase (ACS) Biolistics [9,74] Plant-based vaccine Antituberculosis vaccine ESAT6 of tuberculosis Agrobacterium [81] Anticysticercosis vaccine Synthetic peptide genes (KETc1, KETc12, KETc7) Biolistics [82,84] Table 1.
Summary of transgenic studies in papaya genetic improvement.
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