Figures (1)  Tables (1)

    • Figure 1. 

      Efficient sugarcane transgenic system based on herbicide screening. (a) Original explant from sugarcane tillers for callus induction. (b) Two weeks after first induction through the addition of a high concentration (2 mg/L) of 2,4-D to the medium. (c) Two to three weeks after third induction through the addition of a low concentration (1 mg/L) of 2,4-D and a very low concentration (0.1 mg/L) of 6-BA to the medium. (d) Schematic of the pCAMBIA3300-CFP plant expression vector. RB: Right border of pCAMBIA3300; LB: Left border of pCAMBIA3300; Ubi1: maize Ubi 1 promoter; CFP: CFP visible marker gene; tNOS: nopaline synthase terminator; 35S: cauliflower mosaic virus 35S promoter; bar: bar selective marker gene; 35S poly A: cauliflower mosaic virus 35S poly A tail. (e) Fluorescence observation of infected calluses after 7 d of resting cultivation. (f) Fluorescence observation of resistant calluses after 30 d of callus screening. (g) Fluorescence observation of leaves after 14 d of regeneration, Left: leaf of transgenic shoot, Right: leaf of wild-type shoot. (h) Resistant shoots on the rooting medium. (i) Molecular assay for the bar selective marker gene by traditional PCR; CK−: nontransformation shoots; CK+: plant expression vector; and M: DNA marker ladder. (j) PAT/bar protein assay by QuickStix Strips; 1–21: Resistant shoots; CK−: nontransformation shoots. (k) Herbicide tolerance testing of transgenic shoots. GM: transgenic shoots (2.0 mg/mL Basta); WT: Wild-type shoots (0.5 mg/mL Basta). (l) Tenth day after herbicide spraying; GM: transgenic shoots; WT: Wild-type shoots. (m) First day after weed control in the field; Left: Weed control of wild-type shoots by hoeing; Right: Weed control of transgenic shoots by herbicide. (n) Two weeks after weed control in the field; Left: Weed control of wild-type shoots by hoeing; Right: Weed control of transgenic shoots by herbicide. (o) Two months after weed control; Left: Weed control of wild-type shoots by hoeing; Right: Weed control of transgenic shoots by herbicide.

    • Name of
      vector/gene      		Calluses
      used (g)      		Transgenic shoots provided (lines)Target of genetic transformationStrategy of genetic modificationInstitutes servicedDate
      Cry2A217Pest-resistant genesOEHuazhong Agricultural University2017.5
      Cry1C220OE2017.5
      Hc-Pro220Functional gene of yje SCSMV virusOEYangzhou University2019.12
      ScD27220Tiller-associated genesOESugarcane Research Institute, Yunnan Academy of Agriculture Science2018.5
      ScD10225RNAi2019.6
      INV215Sucrose invertase geneOEInstitute of Nanfan & Seed Industry, Guangdong Academy of Science2021.10
      FUG215Haploidy induction geneGE2019.12
      SsWRKY1- OE215Drought resistance-associated genesOEYunnan Agricultural University2020.8
      SsWRKY1-RNAi220RNAi2020.8
      DREB212Drought resistance-associated genesOESouth Subtropical Crops Research Institute, Chinese Academy of Tropical Agriculture Science2020.9
      REMO214OE2020.9
      MYB8i215MYB transcription factorsRNAiFujian Agriculture and Forestry University2020.11
      MYB11i212RNAi2020.11
      ERF99213Ethylene-responsive factorsOE2020.11
      Z6345JAZ transcription factorsOE2021.9
      Z10350OE2021.9
      VSR380Vacuolar sorting receptorsOEYulin Normal University2022.6
      R1335Plant activator polypeptide receptorOE2022.10
      RK1330Ratoon stunting disease-responsive factorsRNAiSugarcane Research Institute, Guangxi Academy of Agriculture Science2022.10
      * OE: Target gene overexpression; RNAi: Target gene suppression by RNAi; GE: Gene mutation by genomic editing.

      Table 1. 

      Technical service using the efficient transgenic system.