Figures (6)  Tables (3)
    • Figure 1. 

      Gene map of the complete chloroplast genome of A. xanthioides. Genes located on the inside and outside of the circle are transcribed clockwise and counterclockwise, respectively. The inner circle indicates the GC and AT content.

    • Figure 2. 

      Comparison of the borders of the LSC, SSC, and IR regions in the genomes of the three Amomum species. Ψ indicates pseudogenes. The number above the gene feature indicates the distance between the ends of genes and border sites. Figure is not drawn to scale.

    • Figure 3. 

      Codon content of 20 amino acids and stop codons in all protein-coding genes of the chloroplast genomes. The histogram (as read from left to right) shows the different numbers of codons found in A. xanthioides, A. villosum and A. longiligulare.

    • Figure 4. 

      Repeat sequences in three Amomum chloroplast genomes. REPuter was used to identify repeat sequences with length ≥ 30 bp and sequences for which ≥ 90% were identified in chloroplast genomes. Different letters are used to indicate forward repeats (F), palindromic repeats (P), reverse repeats (R), and complementary repeats (C). Repeats of different lengths are indicated in different colors.

    • Figure 5. 

      Sequence alignment in the chloroplast genomes of three Amomum species using mVISTA software. The top line shows the genes in order (with the direction of transcription indicated by an arrow). A cut-off of 70% identity was used for the plots. The Y-axis indicates the value of percent identity and ranges from 50% to 100%.

    • Figure 6. 

      Phylogenetic tree constructed using the maximum likelihood (ML) method based on 53 protein-coding genes from 16 species in the Zingiberales clade. Numbers at nodes indicate the values of bootstrap support.

    • No. Group of genes Gene names Amount
      1Photosystem IpsaA, psaB, psaC, psaI, psaJ5
      2Photosystem IIpsbA, psbB, psbC, psbD, psbE, psbF, psbG, psbH, psbI, psbJ, psbK, psbL, psbM, psbN, psbT, psbZ16
      3Cytochrome b/f complexpetA, petB, petD, petG, petL, petN6
      4ATP synthaseatpA, atpB, atpE, atpF*, atpH, atpI6
      5NADH dehydrogenasendhA*, ndhB*(×2), ndhC, ndhD, ndhE, ndhF, ndhG, ndhH, ndhI, ndhJ, ndhK12(1)
      6RubisCO large subunitrbcL1
      7RNA polymeraserpoA, rpoB, rpoC1*, rpoC24
      8Ribosomal proteins (SSU)rps2, rps3, rps4, rps7(×2), rps8, rps11, rps12**(×2), rps14, rps15, rps16*, rps18, rps19(×2)15(3)
      9Ribosomal proteins (LSU)rpl2*(×2), rpl14, rpl16*, rpl20, rpl22, rpl23(×2), rpl32, rpl33, rpl3611(2)
      10Proteins of unknown functionycf1(×2), ycf2(×2), ycf3**, ycf4, ycf15*(×2), ycf68*(×2)10(4)
      11Other genesaccD, clpP**, matK, ccsA, cemA, infA6
      12Transfer RNAs#39/38/39 tRNAs39/38/39(7)
      13Ribosomal RNAsrrn4.5(×2), rrn5(×2), rrn16(×2), rrn23(×2)8(4)
      * Gene with one intron; ** gene with two introns; (×2) indicates the number of the repeat unit is 2. # indicates different amount among A. xanthioides, A. villosum and A. longiligulare.

      Table 1. 

      Gene contents of three Amomum chloroplast genomes.

    • GeneLocationExon I (bp)Intron I (bp)Exon II (bp)Intron II (bp)Exon III (bp)
      atpF#LSC378770/771/771207
      clpP#LSC252627/626/629237908/909/90972
      ndhA#SSC5671,056/1,067/1,054513
      ndhBIR849603783
      rpl16#LSC81,045/1,042/1,038420
      rpl2IR426658396
      rpoC1#LSC1605751/752/739438
      rps12LSC156159156159
      rps16#LSC234703/707/72146
      trnA-UGCIR3880335
      trnI-GAU#IR35/42/3593742/35/42
      trnL-UAA#LSC35563/534/53450
      trnV-UAC#LSC37606/606/60338
      ycf3#LSC138773/773/774249710/711/710135
      # indicates different amount among A. xanthioides, A. villosum and A. longiligulare.

      Table 2. 

      Gene with introns in three Amomum chloroplast genomes and the length of exons and introns.

    • SSR typeRepeat unitAmountRatio (%)
      A. xanthioidesA. villosumA. longiligulareA. xanthioidesA. villosumA. longiligulare
      MonoA/T576258100100100
      DiGA/TC22213.310.511.8
      AT/AT13171586.788.286.7
      TriGCT/AGC11133.325.033.3
      TTC/GAA11133.325.033.3
      GGA/TCC11133.325.033.3
      ATA/TAT0100.025.00.0
      TetraTTAT/ATAA22215.418.218.2
      AAAG/CTTT1117.79.19.1
      ATTT/AAAT22115.418.29.1
      CTAA/TTAG1117.79.19.1
      AGAA/TTCT22215.418.218.2
      CGTA/TACG1117.79.19.1
      CATA/TATG1107.79.10.0
      GAAT/ATTC1117.79.19.1
      TATT/AATA1117.79.19.1
      AATT/AATT1117.79.19.1
      PentaTTAAA/TTTAA11150.050.050.0
      AATCA/TGATT11150.050.050.0
      HexaTGATAG/CGATCA11125.025.050.0
      GAAGAG/CTCTTC11025.025.00.0
      TCCTCT/AGAGGA11025.025.00.0
      TCACTA/TAGTGA11125.025.050.0

      Table 3. 

      SSR types and amounts in three Amomum chloroplast genomes.