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Figure 1.
Maximum likelihood phylogram inferred from 82 taxa and 1401 characters based on ITS, and LSU matrix using GTR+G+I model. ML bootstrap supports (≥ 70%) and Bayesian posterior probability (≥ 0.70) are indicated above the branches or near the nodes as ML/ML/PP. Tree is rooted using Cunninghamella homothallica (CBS 168.53), C. phaeospora (CBS 692.68), and C. bainieri (FSU319). Strains of the new species are in bold and the type species in the dataset are indicated using T. (−) represent bootstrap support lower than 70%.
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Figure 2.
Absidia zygospora MFLUCC 23–0061 (ex-type). (a) Developing sporangium. (b) Simple sporangiophore with sporangium, and subsporangial septation and columella with apical projection. (c) Rhizoids, (d) Swollen hyphae. (e), (j)–(k) Zygospores with unequal suspensors and finger-like appendages. (f)–(g) Sporangiospores. (h) Columella with apical projection. (i) Simple branching of sporangiophores. (l) Front and obverse images of culture in PDA. Scale bars: a–d, f, h, i = 10 µm, e, j–k = 20 µm, g = 5 µm.
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Species name Voucher no. GenBank accession number ITS LSU Absidia abundans CGMCC.3.16255T NR_182590 ON074683 Absidia aguabelensis URM 8213T MW763074 MW762874 Absidia alpina CGMCC 3.16104 OL678133 Absidia ampullacea CGMCC 3.16054 MZ354138 MZ350132 Absidia anomala CBS 125.68T NR_103626 NG_058562 Absidia bonitoensis URM 7889T MN977786 MN977805 Absidia brunnea CGMCC.3.16055 MZ354139 MZ350133 Absidia caatinguensis URM7156T NR_154704 NG_058582 Absidia californica CBS 126.68T − NG_056998 Absidia caerulea NRRLA9483 − − Absidia caerulea CBS 104.08 JN205811 MH866107 Absidia cornuta URM 6100T NR_172976 MN625255 Absidia cuneospora CBS 102.59 JN205819 JN206579 Absidia cylindrospora var. cylindrospora CBS 100.08 JN205822 JN206588 Absidia cylindrospora var. nigra CBS 127.68T − NG_058560 Absidia cylindrospora var. rhizomorpha CBS 153.63T − NG_058563 Absidia edaphica MFLU 20-0415 − MT393986 Absidia edaphica MFLU 20-0416T MT396372 MT393987 Absidia fusca CBS 102.35T NR_103625 NG_058552 Absidia glauca CBS 101.08T NR_111658 MH866105 Absidia heterospora SHTH021 JN942683 JN982936 Absidia heterospora CBS 101.29T − NG_058564 Absidia jindoensis CNUFC-PTI1-2 MF926623 MF926617 Absidia jindoensis CNUFC-PTI1-1T MF926622 MF926616 Absidia koreana EML-IFS45-2 KR030063 KR030057 Absidia koreana EML-IFS45-1T KR030062 KR030056 Absidia macrospora FSU4746 AY944882 − Absidia macrospora CBS 697.68T − NG_058549 Absidia ovalispora HMAS 249158 MW264133 MW264074 Absidia ovalispora CGMCC 3.16018T MW264071 MW264130 Absidia panacisoli SYPF 7183 MF522181 MF522180 Absidia panacisoli CBS 140959T NR_159563 NG_063948 Absidia pseudocylindrospora EML-FSDY6-2 KU923817 KU923814 Absidia pseudocylindrospora CBS 100.62T NR_145276 NG_058561 Absidia psychrophilia FSU4745 AY944874 EU736306 Absidia repens FSU 4726 EU484288 − Absidia repens NRRL1336 − AF113448 Absidia repens CBS 115583T NR_103624 NG_058551 Absidia soli MFLU 20-0413 MT396371 MT393985 Absidia soli MFLU 20-0414T MT396373 MT393988 Absidia spinosa FSU551 AY944887 EU736307 Absidia spinosa FSU552 AY944888 EU736308 Absidia spinosa var. biappendiculata CBS 187.64 − MH870040 Absidia stercoraria EML-DG8-2 KU168829 KT921999 Absidia stercoraria EML-DG8-1T KU168828 KT921998 Absidia zygospora RSPG 214 KC478527 - Absidia zygospora ANG28 DQ914420 - Absidia zygospora MFLUCC 23–0061T OR104965 OR104992 Absidia chinensis CGMCC.3.16056 MZ354140 MZ350134 Absidia cinerea CGMCC.3.16062 MZ354146 MZ350140 Absidia digitula CGMCC 3.16058 MZ354142 MZ350136 Absidia globospora CGMCC.3.16031 MW671537 MW671544 Absidia healeyae UoMAU1 MT436028 MT436027 Absidia jiangxiensis CGMCC 3.16105 OL678134 - Absidia lobata CGMCC 3.16256 ON074690 ON074679 Absidia medulla CGMCC 3.16034 MW671542 MW671549 Absidia montepascoalis CNUFC HT19001T MW473494 MW561560 Absidia multispora URM 8210 MN953780 MN953782 Absidia oblongispora CGMCC 3.16061 MZ354145 MZ350139 Absidia pararepens CCF 6351 MT193670 MT192307 Absidia pernambucoensis URM 7219 MN635568 MN635569 Absidia purpurea CGMCC 3.16106 OL678135 - Absidia radiata CGMCC 3.16257 ON074698 ON074684 Absidia saloaensis URM 8209T MN953781 MN953783 Absidia saloaensis DXL2020 MN953781 MN953783 Absidia sichuanensis CGMCC 3.16258T NR_182589 ON074688 Absidia sympodialis CGMCC 3.16064 MZ354148 MZ350142 Absidia terrestris FMR 15024 LT795004 LT795593 Absidia terrestris FMR 14989T LT795003 LT795005 Absidia turgida CGMCC.3.16032 MW671540 MW671547 Absidia varians CGMCC.3.16065 MZ354149 MZ350143 Absidia virescens CGMCC.3.16066 MZ354150 MZ350144 Absidia xinjiangensis CGMCC.3.16107 OL678136 Absidia yunnanensis CGMCC 3.16259T NR_182591 NG_149054 Absidia zonata CGMCC.3.16033 MW671541 MW671548 Chlamydoabsidia padenii NRRL 2977T − AF113453 Chlamydoabsidia padenii CBS 172.67T NR_153872 JN206586 Cunninghamella bainieri FSU319 − EU736313 Cunninghamella homothallica CBS 168.53 MH857147 NG_058833 Cunninghamella phaeospora CBS 692.68 AF254934 NG_058812 Halteromyces radiatus NRRL6197 − AF157192 Halteromyces radiatus CBS 162.75 NR_145293 NG_057938 Table 1.
Data used for phylogenetic analysis in this study and their corresponding GenBank accession numbers. Type species are denoted by T. Sequences derived in this study are shown in bold.
Figures
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Tables
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