Figures (11)  Tables (2)
    • Figure 1. 

      Phylogenetic analysis of the ApSWEETs from A. polygama, Arabidopsis thaliana, and Vitis vinifera. The Neighbor-joining tree was drawn using MEGA7.0 with 1,000 bootstraps. The roman numbers (I–IV) labeled with various colors indicate different clades: green – Clade I, orange – Clade II, red – Clade III, blue – Clade IV.

    • Figure 2. 

      ApSWEET gene structure of A. polygama.

    • Figure 3. 

      The conserved motif analyses of ApSWEETs proteins.

    • Figure 4. 

      Conserved structural domains of ApSWEETs.

    • Figure 5. 

      Single protein structure of ApSWEETs.

    • Figure 6. 

      Chromosome location of ApSWEET gene family.

    • Figure 7. 

      Collinearity analysis of the ApSWEET gene family.

    • Figure 8. 

      The cis-elements in the promoter sequences of ApSWEETs gene in A. polygama.

    • Figure 9. 

      ApSWEET expression of different tissues.

    • Figure 10. 

      ApSWEET expression of different fruit development stage.

    • Figure 11. 

      ApSWEET expression of different storage periods.

    • Gene IDNumber of amino acidsMolecular weightpIAsp + GluArg + LysInstability indexPredicted location
      ApSWEET127531,0938.17171937.35 (stable)Cell membrane
      ApSWEET224427,049.866.86161635.79 (stable)Cell membrane
      ApSWEET330433,192.129.49193036.79 (stable)Chloroplast
      ApSWEET425429,132.97.61192046.56 (unstable)Cell membrane
      ApSWEET525928,602.29.71132445.53 (unstable)Cell membrane
      ApSWEET623425,895.588.48141635.70 (stable)Cell membrane
      ApSWEET723826,891.19.18131936.22 (stable)Cell membrane
      ApSWEET823726,653.718.87131734.25 (stable)Cell membrane
      ApSWEET925327,530.99.5122329.02 (stable)Cell membrane
      Golgi apparatus
      ApSWEET1025227,529.89.49122025.31 (stable)Cell membrane
      ApSWEET1123625,918.839.2681436.71 (stable)Cell membrane
      ApSWEET1223425,977.879.0391436.26 (stable)Cell membrane
      ApSWEET1323826,391.359.0191438.49 (stable)Cell membrane
      ApSWEET1423326,641.99.36152443.15 (unstable)Cell membrane

      Table 1. 

      Physicochemical properties of SWEET gene family proteins.

    • Alpha
      helix
      Extended
      strand
      Beta turnRandom
      coil
      ApSWEET145.09%16.73%2.18%36.00%
      ApSWEET241.80%22.54%2.87%32.79%
      ApSWEET349.01%15.46%5.26%30.26%
      ApSWEET437.80%18.11%0.79%43.31%
      ApSWEET535.91%21.24%4.63%38.22%
      ApSWEET650.00%21.79%3.85%24.36%
      ApSWEET740.34%21.85%4.20%33.61%
      ApSWEET840.51%21.94%5.91%31.65%
      ApSWEET933.99%25.30%4.35%36.36%
      ApSWEET1046.83%17.06%3.97%32.14%
      ApSWEET1142.37%19.49%5.08%33.05%
      ApSWEET1241.88%20.94%4.27%32.91%
      ApSWEET1346.22%19.33%2.52%31.93%
      ApSWEET1436.48%21.89%3.86%37.77%

      Table 2. 

      Secondary structure analysis of ApSWEET family members.