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Figure 1. 

Relative expression of CmPDS after transient silencing, as determined by RT-qPCR. Different lowercase letters indicate significant differences between different treatments at the same time (p < 0.05).

Figure 2. 

Process for genetic transformation of C. morifolium. (a) Preculture. (b) Coculture. (c) Screening and cultivation. (d) Albino plant. (e) Interalbino plant. (f) Negative control plant.

Figure 3. 

Sequence alignment of CmPDS from transgenic plants along with the WT and the negative control plants.

Figure 4. 

Relative expression of CmTGA1 after transient silencing, as determined by RT-qPCR. Different lowercase letters indicate significant differences between different treatments at the same time (p < 0.05).

Figure 5. 

Process for genetic transformation of C. morifolium. (a) Preculture. (b) Coculture. (c) Delayed cultivation. (d) Screening and cultivation (the red box highlights a resistant bud). (e) Transgenic plant.

Figure 6. 

Sequence alignment of CmTGA1 from transgenic plants compared to the WT.

Figure 7. 

Expression of CmPR1, CmPR2 and CmPR5 genes in WT and CmTGA1 lines after inoculation with P. horiana. (a) Expression of CmPR1. (b) Expression of CmPR2. (c) Expression of CmPR5. Analysis of significant differences was performed using Student's t-test (**, p < 0.01).