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Figure 1.
Protoparmeliopsis zareii. (a) Thallus. (b) Ascus with ascospores. (c) Algal cells. (d) Ascospores. Scale bars: (a) = 1 cm, (b) = 10 μm, (c) = 100 μm, (d) = 5 μm.
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Figure 2.
The phylogenetic tree of the representatives of the family Lecanoraceae including genera Protoparmeliopsis, Lecanora, Rhizoplaca and Protoparmelia based ITS-rDNA marker sequences. All the genera are separated in the form of distinct clades in the tree. The maximum likelihood method is used in this phylogenetic analysis showing Protoparmeliopsis zareii is the same as the Iranian collection. Pakistani collection is labeled with a black circle in the figure.
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Figure 3.
Phylogenetic analysis of Protoparmeliopsis zareii (KHP05 LOR) based on LSU sequences. The evolutionary history was inferred by using the Maximum Likelihood method and Jukes-Cantor model . The tree with the highest log likelihood (–7,214.55) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 26 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. There were a total of 2317 positions in the final dataset. Evolutionary analyses were conducted in MEGAX.
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Lichen Location Accession number ITS Protoparmeliopsis achariana Austria AF070019 Protoparmeliopsis bipruinosa Austria AF159932 Protoparmeliopsis bolcana Ukraine MK672838 Protoparmeliopsis chejuensis South Korea MK672839
MK672840Protoparmeliopsis zareii Pakistan KHP05 ITS Lecanora garovaglii Austria
USA
USA
Poland
Poland
IranAF189718
KT453728
KU934537
MK084624
MK084626
MK672841Protoparmeliopsis kopachevskae Korea
South Korea
South KoreaMK672845
MK672846
MK672847Protoparmeliopsis laatokkensis China MN912366 Protoparmeliopsis muralis Austria
Romania
Germany
USA
Russia
PolandKC791770
KP059048
KT818623
KU934555
KU934560
KY379232Protoparmeliopsis nashii Austria AF159931 Protoparmeliopsis peltata USA
Iran
Kazakhstan
RussiaKT453722
KT453723
KU934746
KU934751Protoparmeliopsis pseudogyrophorica China MK672851 Protoparmeliopsis zareii Iran KP059049 Protoparmeliopsis sp. Russia KU934865 Protoparmelia badia Germany KF562191 Protoparmelia badia Austria AF070023 Lecanora sp China MW590816 Lecanora muralis Austria AF070015 Lecanora muralis Austria AF159922 Protoparmeliopsis laatokkensis China MT875031 Protoparmeliopsis laatokkensis China MN912366 Protoparmeliopsis nashii Czech Republic ON447553 Lecanora garovaglii Austria AF189718 Rhizoplaca peltata China AY530887 Rhizoplaca peltata China AY509802 Rhizoplaca melanophthalma USA JX948289 Rhizoplaca melanophthalma USA JX948294 Rhizoplaca subdiscrepans China AY509789 Rhizoplaca chrysoleuca USA HM577252 Lecanora perpruinosa Austria AF070025 Lecanora contractula Austria AF070032 Myriolecis contractula USA HQ650604 Lecanora intricata Austria AY398703 Lecanora intricata Austria AF070022 Lecanora polytropa USA HQ650643 Lecanora polytropa Korea DQ534470 Rhizocarpon geminatum China KP314320 Protoparmelia picea Germany KF562194 Protoparmelia montagnei Spain AF101277 Protoparmelia montagnei Spain AF101275 Lecanora achroa USA JN943715 Lecanora achroa USA JN943719 Lecanora tropica USA JN943718 Lecanora tropica USA JN943720 Lecanora caesiorubella USA JN943722 Lecanora caesiorubella USA JN943727 Lecanora farinacea Austria AY541261 Lecanora farinacea Austria AY541262 Lecanora flavopallida USA JN943724 Lecanora carpinea Austria AY541249 Lecanora carpinea Austria AY541248 Lecanora allophana Austria AF070031 Lecanora allophana Austria AF159939 Xanthoria alfredii Sweden EU681345 Xanthoria ulophyllodes Sweden EU681341 Xanthomendoza fallax Sweden EU681346 Oxneria fallax USA MZ922253 Table 1.
ITS sequences included in the recent phylogenetic analysis are mentioned with GenBank accession numbers and country of the taxa.
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Lichen Location Accession number LSU Lecanora achroa Thailand
AustraliaJN939502
JN939527
JN939504Lecanora tropica Thailand
Fiji
KenyaJN939518
JN939533
JN939537Lecanora caesiorubella USA
Australia
AustraliaJN939509
JN939506
JN939508Lecanora farinacea Australia
AustraliaJN939511
JN939513Lecanora flavopallida Australia JN939514
JN939516Lecanora intricata Sweden DQ787345 Lecanora polytropa USA DQ986792 Lecanora sulphurea Sweden DQ787355 Lecanora carpinea Sweden DQ787363 Lecanora aff. Sweden AY853376 Lecanora campestris Sweden DQ787361 Lecnora contractula USA DQ986746 Lecanora perpruinosa Sweden DQ787343 Protoparmeliopsis achariana Sweden
USADQ787341
DQ973027Lecanora muralis Germany HQ660533 Lecanora muralis Sweden DQ787339 Table 2.
LSU sequences used in the phylogentic analysis mentioned with acession numbers and type locality.
Figures
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Tables
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