Figures (3)  Tables (2)
    • Figure 1. 

      Protoparmeliopsis zareii. (a) Thallus. (b) Ascus with ascospores. (c) Algal cells. (d) Ascospores. Scale bars: (a) = 1 cm, (b) = 10 μm, (c) = 100 μm, (d) = 5 μm.

    • Figure 2. 

      The phylogenetic tree of the representatives of the family Lecanoraceae including genera Protoparmeliopsis, Lecanora, Rhizoplaca and Protoparmelia based ITS-rDNA marker sequences. All the genera are separated in the form of distinct clades in the tree. The maximum likelihood method is used in this phylogenetic analysis showing Protoparmeliopsis zareii is the same as the Iranian collection. Pakistani collection is labeled with a black circle in the figure.

    • Figure 3. 

      Phylogenetic analysis of Protoparmeliopsis zareii (KHP05 LOR) based on LSU sequences. The evolutionary history was inferred by using the Maximum Likelihood method and Jukes-Cantor model . The tree with the highest log likelihood (–7,214.55) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained automatically by applying Neighbor-Join and BioNJ algorithms to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach, and then selecting the topology with superior log likelihood value. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. This analysis involved 26 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + Noncoding. There were a total of 2317 positions in the final dataset. Evolutionary analyses were conducted in MEGAX.

    • LichenLocationAccession number ITS
      Protoparmeliopsis acharianaAustriaAF070019
      Protoparmeliopsis bipruinosaAustriaAF159932
      Protoparmeliopsis bolcanaUkraineMK672838
      Protoparmeliopsis chejuensisSouth KoreaMK672839
      MK672840
      Protoparmeliopsis zareiiPakistanKHP05 ITS
      Lecanora garovagliiAustria
      USA
      USA
      Poland
      Poland
      Iran
      AF189718
      KT453728
      KU934537
      MK084624
      MK084626
      MK672841
      Protoparmeliopsis kopachevskaeKorea
      South Korea
      South Korea
      MK672845
      MK672846
      MK672847
      Protoparmeliopsis laatokkensisChinaMN912366
      Protoparmeliopsis muralisAustria
      Romania
      Germany
      USA
      Russia
      Poland
      KC791770
      KP059048
      KT818623
      KU934555
      KU934560
      KY379232
      Protoparmeliopsis nashiiAustriaAF159931
      Protoparmeliopsis peltataUSA
      Iran
      Kazakhstan
      Russia
      KT453722
      KT453723
      KU934746
      KU934751
      Protoparmeliopsis pseudogyrophorica ChinaMK672851
      Protoparmeliopsis zareiiIranKP059049
      Protoparmeliopsis sp.RussiaKU934865
      Protoparmelia badia GermanyKF562191
      Protoparmelia badiaAustriaAF070023
      Lecanora spChinaMW590816
      Lecanora muralisAustriaAF070015
      Lecanora muralisAustriaAF159922
      Protoparmeliopsis laatokkensisChinaMT875031
      Protoparmeliopsis laatokkensisChinaMN912366
      Protoparmeliopsis nashiiCzech RepublicON447553
      Lecanora garovagliiAustriaAF189718
      Rhizoplaca peltataChinaAY530887
      Rhizoplaca peltataChinaAY509802
      Rhizoplaca melanophthalmaUSAJX948289
      Rhizoplaca melanophthalmaUSAJX948294
      Rhizoplaca subdiscrepansChinaAY509789
      Rhizoplaca chrysoleucaUSAHM577252
      Lecanora perpruinosaAustriaAF070025
      Lecanora contractulaAustriaAF070032
      Myriolecis contractulaUSAHQ650604
      Lecanora intricataAustriaAY398703
      Lecanora intricataAustriaAF070022
      Lecanora polytropaUSAHQ650643
      Lecanora polytropaKoreaDQ534470
      Rhizocarpon geminatumChinaKP314320
      Protoparmelia piceaGermanyKF562194
      Protoparmelia montagneiSpainAF101277
      Protoparmelia montagneiSpainAF101275
      Lecanora achroaUSAJN943715
      Lecanora achroaUSAJN943719
      Lecanora tropicaUSAJN943718
      Lecanora tropicaUSAJN943720
      Lecanora caesiorubellaUSAJN943722
      Lecanora caesiorubellaUSAJN943727
      Lecanora farinaceaAustriaAY541261
      Lecanora farinaceaAustriaAY541262
      Lecanora flavopallidaUSAJN943724
      Lecanora carpineaAustriaAY541249
      Lecanora carpineaAustriaAY541248
      Lecanora allophanaAustriaAF070031
      Lecanora allophanaAustriaAF159939
      Xanthoria alfrediiSwedenEU681345
      Xanthoria ulophyllodesSwedenEU681341
      Xanthomendoza fallaxSwedenEU681346
      Oxneria fallaxUSAMZ922253

      Table 1. 

      ITS sequences included in the recent phylogenetic analysis are mentioned with GenBank accession numbers and country of the taxa.

    • LichenLocationAccession number LSU
      Lecanora achroaThailand
      Australia
      JN939502
      JN939527
      JN939504
      Lecanora tropicaThailand
      Fiji
      Kenya
      JN939518
      JN939533
      JN939537
      Lecanora caesiorubellaUSA
      Australia
      Australia
      JN939509
      JN939506
      JN939508
      Lecanora farinaceaAustralia
      Australia
      JN939511
      JN939513
      Lecanora flavopallidaAustraliaJN939514
      JN939516
      Lecanora intricataSwedenDQ787345
      Lecanora polytropaUSADQ986792
      Lecanora sulphureaSwedenDQ787355
      Lecanora carpineaSwedenDQ787363
      Lecanora aff.SwedenAY853376
      Lecanora campestrisSwedenDQ787361
      Lecnora contractulaUSADQ986746
      Lecanora perpruinosaSwedenDQ787343
      Protoparmeliopsis acharianaSweden
      USA
      DQ787341
      DQ973027
      Lecanora muralisGermanyHQ660533
      Lecanora muralisSwedenDQ787339

      Table 2. 

      LSU sequences used in the phylogentic analysis mentioned with acession numbers and type locality.