Figures (8)  Tables (2)
    • Figure 1. 

      Proposed hypothetical picture of the reduction mechanism of copper sulphate by the aqueous leaf extract solution of M. indica on a hot plate at 80 °C for 4 h at 200 rpm.

    • Figure 2. 

      FTIR spectra of M. indica mediated CuO.NPs. FTIR showed corresponding peaks 3,404.13, 300.37, 2,382.35, 1,641.84, 1,476.90, 1,072.42, 872.66, and 651.51cm1 corresponding to various functional groups.

    • Figure 3. 

      XRD spectra of M. indica mediated CuO.NPs. The XRD spectra of the green synthesized M. indica mediated CuO.NPs exhibit seven main peaks at 2θ angles of 49.67, 39.36, 36.75, 29.56, and 25.34 plains respectively.

    • Figure 4. 

      TEM analysis of green synthesized M. indica CuO.NPs. Spherical shape with a diameter ranging between 30−90 nm.

    • Figure 5. 

      In-vitro antibacterial activity of CuO.NPs were evaluated at different doses (30, 60, and 100 µg·mL−1) against E. coli and S. aureus.

    • Figure 6. 

      Fruit detached antifungal in vivo assay. Persimmon fruits were infested and treated with different concentrations of CuO.NPs including: (a) 30 mg·L−1, (b) 60 mg·L−1, (c) and (d) 100 mg·L−1. Control fruit were without any NP.

    • Figure 7. 

      Antioxidant activity of M. indica CuO.NPs. The DPPH assay was carried out with 20, 40, 80, 160, and 320 µg·mL−1 concentrations of M.indica. CuO.NPs and Ascorbic acid were used as a standard.

    • Figure 8. 

      Expected antimicrobial mechanism of CuO.NPs. Disruption of cell wall and cytoplasmic membrane nanoparticles adhere to or pass through cell wall and cytoplasmic membrane. Cu ions denature ribosomes and inhibit protein synthesis. Interruption of adenosine triphosphate (ATP) production: ATP production is terminated because Cu ions deactivate respiratory enzymes on the cytoplasmic membrane. Reactive oxygen species (ROS) produced by the broken electron transport chain can cause membrane disruption. Cu and reactive oxygen species bind to deoxyribonucleic acid and prevent its replication and cell multiplication. CuO.NPs directly move across the cytoplasmic membrane, which can release organelles from the cell.

    • S.
      No.
      Functional groupsCompoundsWave.
      no.
      VibrationBondingPeaks
      1OHAlcohol3,404.13StretchingStrongBroadband
      2O-HCarboxylic300.37StretchingStrongBroadband
      3O=C=OCarbon oxide2,382.35StretchingMediumBroadband
      4−C=CAlkanes1,641.84StretchingMediumBroadband
      5N-HAmine1,476.90BendingMediumBroadband
      6N-HAnhydride1,072.42StretchingStrongBroadband
      7C-ClAldehyde872.66BendingMediumBroadband
      9C-BrAlkyl Halides651.51StretchingStrongBroadband

      Table 1. 

      Possible functional groups in M. indica plant extract.

    • Treatment Diseased area (mm)
      30 mg·L−164.6 ± 1.6
      60 mg·L−144.2 ± 0.6
      100 mg·L−123.4 ± 1.8
      Control97.0 ± 0.81

      Table 2. 

      Effectiveness of green CuO.NPs on fruit against disease.