Figures (8)  Tables (2)

    • Figure 1. 

      Developmental status of pUC-MSC. (a) Cell morphology of pUC-MSC cultured at different passages (× 100) (bar = 300 μm); (b) Growth curve of pUC-MSC at passages 3, 6, and 9; (c) Relative growth rate. Different symbols (*, **, ***, ns) indicate significant differences among groups (p < 0.05).

    • Figure 2. 

      Characterization pUC-MSC (a) Immunofluorescence and observation of cells incubated with CD90; DAPI is used for nuclear staining (b) Proportion of CD90 positive cells; (c) Flow cytometry for surface antigen marker (bar = 100 μm). Different symbols (*, **, ***, ns) indicate significant differences among groups (p < 0.05). (d) Flow cytometry analysis of pUC-MSCs; Dot-plot analysis expressing positive MSC-specific antigens CD90.

    • Figure 3. 

      The overall distribution of transcripts in three data sets. (a) Pearson correlation heatmap showing the correlation between all sample data sets. (b) Volcano plot of significant DEGs detected from three data sets. (c) Heatmaps show the overall distribution of DEGs in respective data sets.

    • Figure 4. 

      KEGG enrichment analysis of DEGs in the different comparison groups, where the X-axis represents DEG’s numbers and the Y-axis represents the pathway. (a) P6 vs P4, (b) P9 vs P4, and (v) P11 vs P4.

    • Figure 5. 

      GO enrichment analysis of DEGs in different comparison group, where the X-axis represents the number of DEGs and the Y-axis represents different GO terms. (a) P6 vs P4, (b) P9 vs P4, and (c) P11 vs P4.

    • Figure 6. 

      Comparison of P4, P6, P9, and P11 transcriptome profiles. The cluster heatmap analysis of differentially expressed related to (a) stemness, (b) cell differentiation, (c) metabolic pathways, and (d) cells cellular senescence.

    • Figure 7. 

      Expression levels of pluripotency-related genes in pUC-MSC at P4, P6, P9, and P11. (a) APC, (b) MYC, (c) KRAS, (d) DPPA4, (e) SMAD5. Different symbols (a, b, c, d) which are on the top of each column indicate significant differences among groups (p < 0.05).

    • Figure 8. 

      Expression levels of differentiation related genes in pUC-MSC at P4, P6, P9, and P11. (a) NOTCH3, (b) MAPK8, (c) OCT4, (d) SOX2, (e) NANOG. Different symbols (a, b, c, d) which are on the top of each column indicate significant differences among groups (p < 0.05).

    • Gene name Gene ID Primer sequence Product length (bp)
      MYCNM_001005154.1F: AGCACAATTATGCAGCGCCC80
      R: GACCCTGCCACTGTCCAACT
      KRASXM_013993793.2F: GGGAGAGAGGCCTGCTGAAA71
      R: ACTCTTGCCTACGCCACCAG
      APCNM_001206430.1F: TGGCAACTTCGGGTAACGGT99
      R: GCCTTCGAGGAGCAGAGTGT
      DPPA4XM_005654065.3F: ACCGGCCAACCTGATTCACA81
      R: TCCAGTTTCCGGCCTTTGGT
      GAPDHXM043245356.3F: TCGGAGTGAACGGATTTGGC
      R: TGCCGTGGGTGGAATCATAC
      NOTCH3XM_021083631.1F: ATGGTCTTCCCTTACCACCG108
      R: ACGGTTGTCAATCTCCAGCA
      MAPK8XM_021073087.1F: CAGTCTCCACCGCCTAGGTT75
      R: GATCCCTCGCTGCTACCTGG
      OCT4MF955857.1F: GGCTCCCCCATGCATTCAAA80
      R: TCTCTCCCTAGCTCACCCCTT
      SOX2NM_001123197.1F: GAGCGCCCTGCAGTACAACT87
      R: CCCTGCTGCGAGTAGGACAT
      NANOGEF522119.1F: ACGGTGGACCTGCAAGTAGT92
      R: GCTGCTGAGTAACCCAGACT

      Table 1. 

      Primer sequences of target genes in qRT-PCR.

    • Sample Reads number Base number (bp) Q20 (%) Q30 (%)
      MPS-P4-1 39,132,268 5,830,212,150 98.22 94.95
      MPS-P4-2 39,384,712 5,894,408,375 98.41 95.34
      MPS-P4-3 39,323,628 5,887,845,684 98.31 95.17
      MPS-P6-1 39,279,880 5,877,249,370 98.17 94.75
      MPS-P6-2 39,267,936 5,879,963,739 98.17 94.79
      MPS-P6-3 39,248,876 5,872,768,921 98.12 94.67
      MPS-P9-1 39,230,388 5,870,955,649 98.06 94.53
      MPS-P9-2 39,317,102 5,885,981,291 98.3 95.12
      MPS-P9-3 39,102,492 5,853,218,947 98.18 94.91
      MPS-P11-1 39,078,322 5,849,103,924 98.25 94.97
      MPS-P11-2 39,196,888 5,868,142,702 98.02 94.32
      MPS-P11-3 38,951,882 5,831,153,453 98.32 95.18

      Table 2. 

      Mapping results with pig genome.