-
Figure 1.
Maximum likelihood phylogenetic tree generated by RAxML based on a concatenated ITS and LSU sequence data. The tree is rooted to Cunninghamella blakesleeana (CBS 782.68) and C. elegans (CBS 167.53). Maximum likelihood bootstrap values (MLBS) greater than 70% and Bayesian posterior probabilities (BYPP) greater than 0.95 are noted above the nodes as MLBS/BYPP. Ex-type strains are in bold and newly generated sequences are in blue.
-
Figure 2.
Absidia menglianensis (KUN-HKAS 144524, holotype). (a), (b) Culture from above and reverse; (c) Sporangiospores; (d) Sporangium; (e), (f) Sporangia with columellae (arrow); (g) Rhizoids; (h) Swelling on sporangiophores; (i), (j) Sporangiophores. Scale bars: (c), (f) = 10 μm, (d) = 15 μm, (e) = 7 μm, (g) = 20 μm, (h), (j) = 50 μm, (i) = 30 μm.
-
Figure 3.
Mycelial growth of Absidia menglianensis in PDA at different temperatures (32, 34, 35, 36 °C) after 3 d. (a)–(d) Colonies at 32 °C; (e)–(h) Colonies at 34 °C; (i)–(l) Colonies at 35 °C; (m)–(p) Colonies at 36 °C.
-
Species names Strain numbers GenBank accession numbers ITS LSU Absidia abundans XY09265 ON074697 ON074681 Absidia abundans CGMCC 3.16255 NR_182590 ON074683 Absidia abundans XY09274 ON074696 ON074682 Absidia aguabelensis URM 8213 NR_189383 NG_241934 Absidia alpina CGMCC 3.16104 OL678133 / Absidia ampullacea CGMCC 3.16054 NR_191130 NG_242502 Absidia anomala CBS 125.68 NR_103626 NG_058562 Absidia biappendiculata CBS 187.64 NR_190243 MZ350147 Absidia bonitoensis URM 7889 MN977786 MN977805 Absidia brunnea CGMCC 3.16055 NR_191131 NG_242503 Absidia caatinguensis URM 7156 NR_154704 NG_058582 Absidia caerulea CBS 101.36 MH855718 MH867230 Absidia caerulea XY00608 OL620081 / Absidia californica CBS 126.68 NR_077169 NG_056998 Absidia californica FSU4748 AY944873 EU736301 Absidia cheongyangensis CNUFC CY2203 PP844904 PP852788 Absidia chinensis CGMCC 3.16057 MZ354141 MZ350135 Absidia chinensis CGMCC 3.16056 NR_191132 NG_242504 Absidia cinerea CGMCC 3.16062 NR_191134 NG_242506 Absidia coerulea FSU767 AY944870 / Absidia coerulea XY00729 OL620082 / Absidia cornuta URM 6100 NR_172976 MN625255 Absidia crystalloides CGMCC3.27496 PP377803 PP373736 Absidia crystalloides SAUCC693201 PP377804 PP373737 Absidia cuneospora CBS 101.59 MH857828 NG_058559 Absidia cylindrospora CBS 153.63 / NG_058563 Absidia cylindrospora XY00313 ON123744 ON123765 Absidia cylindrospora var. cylindrospora CBS 100.08 JN205822 JN206588 Absidia digitula CGMCC 3.16058 MZ354142 MZ350136 Absidia edaphica MFLU 20-0416 NR_172305 NG_075367 Absidia fluvii CNUFC CY2240 PP844891 PP852703 Absidia frigida CGMCC 3.16201 NR_182565 NG_149032 Absidia fusca CBS 102.35 NR_103625 NG_058552 Absidia gemella CGMCC 3.16202 OM108488 NG_149033 Absidia glauca CBS 129233 MH865253 MH876693 Absidia glauca CBS 101.08 NR_111658 NG_058550 Absidia glauca FSU660 AY944879 EU736302 Absidia globospora CGMCC 3.16031 NR_189829 NG_241930 Absidia globospora CGMCC 3.16035 MW671538 MW671545 Absidia globospora CGMCC 3.16036 MW671539 MW671546 Absidia healeyae UoMAU1 MT436028 MT436027 Absidia heterospora SHTH021 JN942683 JN982936 Absidia jiangxiensis CGMCC 3.16105 OL678134 / Absidia jiangxiensis [as jindoensis] XY000810 ON123748 ON123770 Absidia jindoensis CNUFC-PTI1-1 MF926622 MF926616 Absidia koreana EML-IFS45-1 KR030062 KR030056 Absidia kunryangriensis CNUFC CY2230 PP844905 PP956882 Absidia lobata CGMCC 3.16256 ON074690 ON074679 Absidia longissima CGMCC 3.16203 NR_182566 OM030225 Absidia macrospora FSU4746 AY944882 EU736303 Absidia medulla CGMCC 3.16034 NR_189832 NG_241932 Absidia menglianensis KUNCC 24-18541 PQ594927 PQ594929 Absidia menglianensis KUNCC 24-18542 PQ594928 PQ594930 Absidia microsporangia KUNF-22-121A OQ868364 OQ868366 Absidia microsporangia KUNF-22-126A OQ868365 OQ868367 Absidia microsporangia KUNF-22-316 PP264375 PP264469 Absidia montepascoalis URM 8218 MW473494 MW561560 Absidia multispora URM 8210 MN953780 MN953782 Absidia nigra CGMCC 3.16060 MZ354144 MZ350138 Absidia nigra CGMCC 3.16059 MZ354143 MZ350137 Absidia nigra CBS 127.68 NR_173068 NG_058560 Absidia oblongispora CGMCC 3.16061 NR_191133 NG_242505 Absidia ovalispora CGMCC 3.16019 NR_176748 MW264131 Absidia pacifica CGMCC3.27497 PP377802 PP373735 Absidia pacifica SAUCC413601 PP377801 PP373734 Absidia panacisoli SYPF 7183 NR_159563 NG_063948 Absidia paracylindrospora CNUFC L2207 PP844907 PP956883 Absidia pararepens CCF 6352 MT193669 NG_242483 Absidia pateriformis CGMCC3.27495 PP377805 PP373738 Absidia pateriformis SAUCC634702 PP377806 PP373739 Absidia pernambucoensis URM 7219 MN635568 MN635569 Absidia pseudocylindrospora CBS 100.62 NR_145276 NG_058561 Absidia psychrophilia FSU4745 AY944874 EU736306 Absidia psychrophilia CBS 128.68 NR_154667 NG_058544 Absidia purpurea CGMCC 3.16106 NR_191153 / Absidia radiata CGMCC 3.16257 ON074698 ON074684 Absidia radiata XY09330 ON074699 ON074685 Absidia repens CBS 115583 NR_103624 NG_058551 Absidia saloaensis URM 8209 MN953781 MN953783 Absidia sichuanensis CGMCC 3.16258 NR_182589 ON074688 Absidia soli MFLU 20-0414 NR_172306 NG_075368 Absidia sp. [as pseudocylindrospora] EML-FSDY6-2 KU923817 KU923814 Absidia spinosa FSU551 AY944887 EU736307 Absidia stercoraria EML-DG8-1 KU168828 KT921998 Absidia sympodialis CGMCC 3.16063 MZ354147 MZ350141 Absidia sympodialis CGMCC 3.16064 NR_191135 NG_242507 Absidia tarda URM 8412 PP844911 PP956884 Absidia terrestris FMR 14989 LT795003 LT795005 Absidia thailandica MFLUCC 23-0073 OR606547 OR606546 Absidia turgida CGMCC 3.16032 NR_189830 NG_241931 Absidia varians CGMCC 3.16065 NR_191136 NG_242508 Absidia variiprojecta URM 8620 PP844913 PP956885 Absidia variispora URM 8720 PP844915 PP956886 Absidia virescens CGMCC 3.16066 MZ354150 MZ350144 Absidia virescens CGMCC 3.16067 NR_191137 NG_242509 Absidia xinjiangensis CGMCC 3.16107 OL678136 / Absidia yunnanensis XY09528 ON074701 ON074686 Absidia yunnanensis CGMCC 3.16259 NR_182591 NG_149054 Absidia zonata CGMCC 3.16033 NR_189831 NG_242490 Absidia zygospora MFLUCC 23-0061 OR104965 OR104992 Cunninghamella blakesleeana CBS 782.68 JN205869 MH870950 Cunninghamella elegans CBS 167.53 MH857146 HM849700 The ex-type strains are in bold and newly sequences in the present study are indicated in blue. Abbreviations: CBS: Westerdijk Fungal Biodiversity Institute, Utrecht, Netherlands; CCF: Culture Collection of Fungi, Charles University in Prague; CGMCC: China General Microbiological Culture Collection Center, Beijing, China; CNUFC: Chonnam National University Fungarium, Gwangju, South Korea; FMR: Facultad de Medicina, Reus, Tarragona, Spain; KUNCC: Kunming Institute of Botany Culture Collection, Kunming, China; MFLU: Herbarium of Mae Fah Luang University, Chiang Rai, Thailand; MFLUCC: Mae Fah Luang University Culture Collection, Chiang Rai, Thailand; NRRL: USDA-Agricultural Research Service Culture Collection, US; SAUCC: Shandong Agricultural University Culture Collection (SAUCC), China; URM: University Recife Mycology Culture Collection of the Universidade Federal de Pernambuco, Brazil. Table 1.
Taxa names, strain numbers, and GenBank accession numbers of taxa used for the present phylogenetic analyses.
Figures
(3)
Tables
(1)