Figures (4)  Tables (6)

    • Figure 1. 

      Sampling localities of six yellow catfish populations.

    • Figure 2. 

      Gene flow (Nm) analysis of six yellow catfish populations. (a) Matrix of pair-wise estimated gene flow (Nm) among six yellow catfish populations; (b) Linear regression analysis of gene flow (Nm) and geographic distance.

    • Figure 3. 

      Population and individual clustering analysis of six yellow catfish populations. (a) Genetic relatedness of 124 individuals depicted by a circular phylogenetic tree; (b) Principal coordinates analysis (PCoA) plot of 124 individuals in six yellow catfish populations; (c) UPGMA dendrogram based on Nei's genetic distance among six yellow catfish populations.

    • Figure 4. 

      Ancestral lineage analysis of six yellow catfish populations. (a) Delta K, (b) MedMed K / MedMean K, and (c) MaxMed K / MaxMean K plots indicating the best number of clusters (K) of the STRUCTURE analysis. (d) Cluster assignment from STRUCTURE averaged over six replicated runs for K = 3. Colors represent different clusters.

    • Population Collection date Geographic locations Sample size
      DTHL 2018-4-6 Hunan (113°0′58″ E, 29°23′35″ N) 21
      GHL 2018-6-13 Jiangsu (119°53′35″ E, 31°38′1″ N) 21
      HHL 2018-4-8 Hubei (113°28′40″ E, 29°52′44″ N) 21
      HZHL 2018-6-9 Jiangsu (118°49′12″ E, 33°21′36″ N) 20
      PYHL 2018-4-10 Jiangxi (116°38′7″ E, 29°10′13″ N) 21
      WSLR 2018-9-21 Heilongjiang (133°41′7″ E, 45°59′20″ N) 20

      Table 1. 

      Sampling information of six populations of yellow catfish.

    • Locus Chromosome Location Primer sequences (5'-3') Tm ( °C) Label Size Ref.
      AG12 15 5859684…5859993 F: TTCTGAGGGGATGGTG 52 FAM 255−335 [10]
      R: GCGGTGCTCTGTGGTTGTC
      AG128 17 22452450...22452570 F: AAACCGACGGGACAAAAGAT 53 HEX 104−140
      R: CTCTGCCTCACTAACT
      CT81 9 22532293…22532445 F: GTCTCCATCACTGCCACAT 55 FAM 135−161
      R: TCAGCAATTATGTGAAAAGTGTCT
      PF01 1 3380349…3380609 F: TCTGCTGTGCCACCTTCATCT 54 HEX 254−280 [15]
      R: GGCAGGTTCCAGCCGTCAATA
      PF03 23 3321850…3322202 F: GCAACAACAGAACGAAAAAAG 55 FAM 346−354
      R: ACGCTACACAGCAGAGGAAGT
      PF05 7 11753992…11754513 F: GAGTAAGTAAGTGATAATATGGAG 56 HEX 467−527
      R: TCCTACTGTCTTTCACTCTACT
      PF08 20 18894877…18895050 F: AACATCACATTGTTTCTCACCATCC 56 FAM 149−227
      R: AAAGTGAAAGAGAAGTTACGGAGGC
      PF130 10 18287250…18287472 F: CTTTGTAACTGGTATCGTTCTCTAC 55 HEX 203−277
      R: TGCTATTGCTAAAGAGGGAGA
      PF448 19 2770597…2770941 F: TGCTCGAGTGAGCTACGA 54 FAM 312−392
      R: CATCTCTATCTTTTTATTTCACATT
      PF532B 6 28255806…28255978 F: CGTCGTATCACCTGAAAT 53 HEX 134−200
      R: TGAGAGGAGGAGTCTTTGA

      Table 2. 

      Sequence of the primers used to amplify SSR markers.

    • Populations Na Ne Ho He PIC I
      WSLR (n = 20) 9.000 3.764 0.614 0.588 0.569 1.425
      PYHL (n = 21) 10.700 6.715 0.622 0.714 0.696 1.818
      GHL (n = 21) 10.100 6.052 0.662 0.684 0.665 1.717
      HHL (n = 21) 11.200 6.997 0.661 0.727 0.710 1.872
      HZHL (n = 20) 11.500 7.292 0.632 0.695 0.682 1.849
      DTHL (n = 21) 10.300 6.619 0.667 0.705 0.685 1.765

      Table 3. 

      Genetic polymorphic parameters of six yellow catfish populations.

    • Locus WSLR PYHL GHL HHL HZHL DTHL
      n = 20 n = 21 n = 21 n = 21 n = 20 n = 21
      AG12 1.000 0.186 0.801 0.693 0.614 0.260
      AG128 1.000 0.016* 0.032* 0.198 0.597 0.931
      CT81 1.000 0.991 0.967 1.000 1.000 0.773
      PF01 1.000 0.098 0.345 0.790 0.002** 0.774
      PF03 0.305 0.780 0.685 0.605 0.581 0.514
      PF05 0.988 0.995 0.381 0.000*** 0.000*** -
      PF08 0.995 0.805 0.085 0.101 0.372 0.707
      PF130 0.772 0.919 0.761 0.062 0.968 0.988
      PF448 0.000*** 0.000*** 0.000*** 0.000*** 0.001** 0.003**
      PF532B 0.987 0.058 1.000 0.411 0.070 0.024*
      * Indicates a deviation from Hardy-Weinberg equilibrium (p < 0.05); ** Indicates significant deviation from Hardy-Weinberg equilibrium (p < 0.01); *** Indicates very significant deviation from Hardy-Weinberg equilibrium (p < 0.001).

      Table 4. 

      Hardy-Weinberg equilibrium chi-square test in six yellow catfish populations.

    • Source of variation df Sum of squares Variance components Percentage
      of variation
      Among populations 5 5.362 0.015 3%
      Among individuals 118 54.876 0.027 6%
      Within individuals 124 51.000 0.411 91%
      Total 247 111.238 0.453 100%

      Table 5. 

      Molecular variance analysis (AMOVA) of six yellow catfish populations.

    • WSLR PYHL GHL HHL HZHL DTHL
      WSLR − 0.054 0.069 0.059 0.042 0.066
      PYHL 0.324 − 0.027 0.014 0.028 0.019
      GHL 0.261 0.135 − 0.033 0.025 0.043
      HHL 0.281 0.084 0.181 − 0.029 0.020
      HZHL 0.254 0.157 0.134 0.166 − 0.051
      DTHL 0.305 0.098 0.190 0.109 0.231 −

      Table 6. 

      Matrix of pair-wise Nei's genetic distance (below diagonal) and Fst values (above diagonal) among six yellow catfish populations.