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Figure 1. 

Structural and functional features of the M. figo mitogenome. (a) The raw assembly graph of the M. figo mitogenome. (b) The disentangled graph of the M. figo mitogenome. (c) Circular mitogenome map of M. figo. Genes depicted outside the outer circle undergo clockwise transcription, while those positioned within the inner circle undergo counter-clockwise transcription. The legends of different colors positioned in the bottom left corner serve to distinguish genes based on their specific functionalities.

Figure 2. 

Codon usage of four Magnoliaceae mitogenomes. (a) Stacked column plots of the relative synonymous codon usage. (b) Heatmap of the codon usage frequencies.

Figure 3. 

The distribution of repeats in the M. figo mitogenome. From the center outward, the first circle shows the mitogenome of M. figo, the second and third circle shows tandem repeats and simple sequence repeats, respectively. The inner lines represent the dispersed repeats. The legends of different colors positioned in the bottom left corner represent the dispersed repeats of different lengths.

Figure 4. 

(a) Type and number of simple sequence repeats in the mitogenomes of two Magnolia species and M. figo. (b) Length and number of dispersed repeats in the mitogenomes of two Magnolia species and M. figo. (c) The different colored legends indicate different species.

Figure 5. 

(a) Homologous sequences between mitogenome and plastome. The plastome is represented by the green circular segment and the mitogenome by the gray circular segment, and two different kinds of yellow lines represent the homologous fragments. The legends of different colors positioned in the bottom right corner represent fragments of different lengths. (b) Lengths and numbers of these homologous fragments in the M. figo mitogenome.

Figure 6. 

Schematic representation of the collinearity among four Magnoliaceae mitogenomes. (a) Collinearity plots of the four Magnoliaceae mitogenomes. The mitogenomes are shown by the bars in each row, and collinear regions are indicated by the connecting lines in the center. (b) Lengths and numbers of collinear blocks. The different colored legends indicate homologous fragments between different species.

Figure 7. 

The phylogenetic trees constructed based on M. figo and other 14 plant mitogenomes and plastomes. The bootstrap values are clearly displayed within each node. The utilization of distinct colors serves to show the various groups to which the specific species belong. (a) The tree was constructed based on 18 shared mitochondrial genes. (b) The tree was constructed based on 61 shared plastid genes.