Figures (1)  Tables (1)

    • Figure 1. 

      Producing transgene-free gene edited plants by expressing mobile transcripts with A. rhizogenes. Soil-grown or in vitro shoots were infected by A. rhizogenes carrying cassettes to express mobile gene editing components. The expressed mobile components within induced transgenic hairy roots (red) move to the upper portion of the plant, resulting in the desired edits (red). The transgene-free edited plants (red) can be obtained through sexual or asexual methods.

    • Plant A. rhizogenes strain Editing target Regeneration method Ref.
      Chinese cabbage (Brassica rapa) K599 BrPDS Developmental gene/no hormone [18]
      Chicory (Cichorium intybus) 15834 CiPDS Spontaneously shoot from hairy root [19]
      Citrus (Citrus medica) K599 CmACD2 Transgenic hairy root [20]
      Citrus (Citrus sinensis) K599 CsPP2-1 Transgenic hairy root [21]
      Citrus spp. Not specified CsNPR3 Hormone induced (various
      combinations of auxin and cytokinin)      		 [22]
      Cotton (Gossypium hirsutum L.) K599 GhPDS Transgenic hairy root [23]
      Kiwifruit (Actinidia chinensis, Actinidia eriantha) K599 AcCEN4, AeCBL3 Hormone-induced (Zeatin and NAA) [24]
      Medicago truncatula ARqua1 NCR068 Hormone-induced (2,4-D and BAP) [25]
      Populus (Populus tremula × alba) ARqua1 PtaSHR, PtaYUC4/PtaPLT1, PtaLBD4/PtaLBD12 Transgenic hairy root [26]
      potato DMF1 (Solanum tuberosum × chacoense) MSU440 StPDS Hormone-induced
      (Zeatin, IAA and GA3)      		 [27]
      Pyrethrum MSU440 TcEbFS Transgenic hairy root [28]
      Radish (Raphanus sativus L.) MSU440 RsPDS Developmental gene/no hormone [29]
      Russian dandelion (Taraxacum kok-saghyz) K599 TKSPDS Budding from hairy root [10]
      Salvia miltiorrhiza ACCC10060 SmCPS1 Transgenic hairy root [30]
      Soybean (Glycine max) K599 GmNSP1a, GmNSP1b Transgenic hairy root [31]
      Soybean (Glycine max) K599 SACPD-C SMT Transgenic hairy root [32]
      Soybean (Glycine max) K599 GmUOX1 Transgenic hairy root [33]
      Soybean (Glycine max) k599 Glyma06g14180 Transgenic hairy root [34]
      Soybean (Glycine max) K599 GmLHY Transgenic hairy root [35]
      Soybean (Glycine max) K599 Glyma03g36470, Glyma14g04180, Glyma06g14180 Transgenic hairy root [36]
      Succulents (Kalanchoe blossfeldiana) K599 KbPDS Direct bud formation [37]
      Sweet potato (Ipomoea batatas) K599 IbPDS Budding from hairy root [10]
      Tartary buckwheat (Fagopyrum tataricum) ACCC10060 FtMYB45 Transgenic hairy root [38]
      Tea (Camellia sinensis) K599, ATCC15834 MYB73 Hormone-induced (BAP and NAA) [39]
      Tomato (Solanum lycopersicum) ATCC15834 SlSCRpro-mGFP5, SlSHR Transgenic hairy root [40]
      White lupin (Lupinus albus L.) K599 Lalb_Chr05g0223881 Transgenic hairy root [41]

      Table 1. 

      Examples of A. rhizogenes-mediated gene editing in plants.