Figures (1)  Tables (2)

    • Figure 1. 

      Map of Puerto Rico showing the caves included in this study. Modified from Rodríguez-Durán et al.[11].

    • Temperature treatment Number of fungal isolates
      Cueva Culebrones Cueva Matos Cueva Tuna
      25 °C (control) 27 9 18
      75 °C 4 0 4
      90 °C 5 10 11

      Table 1. 

      Fungal isolates recovered from cave soil samples following temperature treatments.

    • Isolate Locationa Heat toleranceb UNITE Blastn annotation
      Species Max. ID percent GenBank accesion number
      MP7-2-25 CC N Arachnotheca glomerata 89.48 MH860158
      MP6-5c-25 CC N Arachnotheca glomerata 88.66 MH860158
      CT3-1-75 CT Y Aspergillus 96.28 MT732784
      CT8-3-25 CT N Aspergillus sydowii 100 PQ637414
      MP1-3-25 CC N Aspergillus tamarii 99.74 PQ804337
      CT8-6-25 CT N Aspergillus terreus 100 PQ789219
      MP6-2-90 CC Y Aspergillus versicolor 99.66 OR259030
      MP6-1-90 CC Y Aspergillus versicolor 100 PQ637403
      MP6-5-25 CC N Blastobotrys mokoenaii 100 OM904991
      CM4-2-90 CM Y Cladosporium xanthochromaticum 99.81 PQ037767
      CT8-2-25 CT N Geosmithia pallida 99.46 MH426779
      CT9-2-90 CT Y Paecilomyces formosus 100 PP191151
      MP9-6-25 CC N Penicillium 100 KX961209
      MP8-1-25 CC N Penicillium 100 PQ268904
      CM5-2-25 CM N Penicillium 100 PQ268904
      B8 CM N Penicillium citrinum 100 UDB023806
      CT2-1-90 CT Y Periconia macrospinosa 100 PP069923
      MP6-1-75 CC Y Talaromyces allahabadensis 100 UDB035160
      CT3-2-90 CT Y Trichoderma 100 PQ784313
      CM5-1-25 CM N Trichoderma virens 99.62 UDB0802761
      a CC = Cueva Culebrones; CM = Cueva Matos; CT = Cueva Tuna.
      b Y = yes; N = no.

      Table 2. 

      Molecular identification and taxonomic annotation of representative fungal isolates using the UNITE database.