Back Article

Figure 1. 

Relative expression of SmMYB44-l in different colored peels and tissues (* p < 0.05, ** p < 0.01; ns: not significant). (a) Relative expression in different colored peels; (b) relative expression in different tissues.

Figure 2. 

Effect of SmMYB44-l overexpression on anthocyanin synthesis in tobacco leaves (** p < 0.01). (a) Total anthocyanin content in tobacco leaves with overexpressed SmMYB44-l; (b) expression levels of relevant structural genes in overexpressed SmMYB44-l tobacco leaves.

Figure 3. 

Effect of SmMYB44-l overexpression on anthocyanin synthesis in transgenic eggplant leaves. (a) Total anthocyanin content in eggplant leaves with overexpressed SmMYB44-l; (b) expression levels of relevant structural genes in overexpressed SmMYB44-l eggplant leaves (** p < 0.01).

Figure 4. 

Expression analysis of SmMYB44-l and SmMYB75 in Nicotiana benthamiana leaves (* p < 0.05, ** p < 0.01). (a) Transient overexpression of SmMYB44-l and SmMYB75 in Nicotiana benthamiana leaves. Numbers in the figure indicate infected genes; (b) total anthocyanin contents in Nicotiana benthamiana leaves agroinfiltrated with different gene constructs.

Figure 5. 

Expression levels of the MYB gene (SmMYB44-l and SmMYB75) and structural genes (NbCHS and NbF3H) in Nicotiana benthamiana leaves (* p < 0.05, ** p < 0.01).

Figure 6. 

Interaction results of SmMYB44-l with the proSmCHS and proSmF3H.

Figure 7. 

DNA–protein interactions were verified by LUC. (a) proSmCHS activity with SmMYB44-l; (b) proSmF3H activity with SmMYB44-l.