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    • Figure 1. 

      Expression analysis and subcellular localization of CsJAZ2 and lnc87821. (a) Expression levels of CsJAZ2 under salt and alkali stress. (b) Expression of lnc87821 and CsJAZ2 were significantly negatively correlated under salt and alkali stress. Data is the mean ± SD. * Indicates significant difference between salt and alkali stress, * p ≤ 0.05, ** p ≤ 0.01. (c) Expression pattern analysis of CsJAZ2 and lnc87821 in different tissues of tea plant. Different letters indicate significant difference between groups, p ≤ 0.05. (d) Subcellular localization reveals the nuclear localization of CsJAZ2, scale bar = 10 μm. (e) Nuclear-cytoplasmic fractionation reveals the nuclear localization of lnc87821. (f) Yeast one-hybrid assay verified the transcriptional activation activity of CsJAZ2.

    • Figure 2. 

      Analysis of the resistance of CsJAZ2-OE Arabidopsis under salt and alkali stresses. (a) The growth condition of CsJAZ2-OE Arabidopsis under salt and alkali stresses. (b) DAB staining of H2O2 in leaves of CsJAZ2-OE Arabidopsis under salt and alkali stresses, scale bar = 1 cm. (c) NBT staining of O2− in leaves of CsJAZ2-OE Arabidopsis under salt and alkali stresses, scale bar = 1 cm. (d) The root growth of CsJAZ2-OE Arabidopsis under salt and alkali stresses, scale bar = 1 cm. (e) Statistics of root length. (f) Statistics of H2O2 content in leaves. (g) Statistics of O2− content in leaves. Data is the mean ± SD, * represents significant difference (p ≤ 0.05), ** represents extremely significant difference (p ≤ 0.01).

    • Figure 3. 

      Transcriptome analysis of CsJAZ2-OE Arabidopsis. (a) Statistics of up- and downregulated DEGs under different treatments. (b) Venn diagram of DEGs under different treatments. (c) Expression heatmap of Arabidopsis JAZ and its downstream genes.

    • Figure 4. 

      AsODN-based suppression of CsJAZ2 and lnc87821 in tea shoots. (a) Effects of CsJAZ2 silencing on the expressions of downstream genes. (b) 6 h-silencing of CsJAZ2 in tea shoots treated with salt and alkali stresses. (c) Determination of H2O2 and MDA contents after salt and alkali treatments. (d) Effects of AsODN inhibition of lnc87821 on expressions of CsJAZ2 and its downstream genes. Data is the mean ± SD, * represents significant difference (p ≤ 0.05), ** represents extremely significant difference (p ≤ 0.01).

    • Figure 5. 

      Prediction and GUS verification of coding potential of lnc87821. (a) Coding potential prediction of lnc87821. (b) GUS verification of coding capability of lnc87821.

    • Figure 6. 

      Prediction of the regulatory mechanism of lnc87821 on CsJAZ2 under salt and alkali stresses. (a) Analysis of cis-acting elements of CsJAZ2 promoter. (b) Position relation of lnc87821, CsJAZ2, and its promoter. (c) Expression clustering analysis of differentially expressed bZIPs and CsJAZ2 under salt and alkali stresses. (d) Expression clustering analysis of differentially expressed bHLHs and CsJAZ2 under salt and alkali stresses.

    • Figure 7. 

      A predicted model for lnc87821-CsJAZ2 participating in the salt and alkali stress responses in tea plant.