Figures (44)  Tables (2)

    • Figure 1. 

      Phylogenetic tree of the genus Apiospora based on the alignment of ITS, LSU, tef1-α, and tub2 sequences, with Arthrinium caricicola serving as the outgroup. The Bayesian inference posterior probability (left, BIPP ≥ 0.90) and the maximum likelihood bootstrap value (right, MLBV ≥ 70%) are shown as BIPP/MLBV above the nodes. Strains marked "*" in the tree are represented as ex-type or ex-epitype. Strains in this study are highlighted in red. The scale bar at the bottom indicates 0.01 substitutions per site. Furthermore, four single-gene trees were evaluated for Apiospora (Supplementary Information 10).

    • Figure 2. 

      Apiospora alpiniae (CGMCC 3.29207, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 3. 

      Apiospora baihualingensis (CGMCC 3.29212, ex-type culture). (a, b) Colonies after incubation on PDA for 7 days; top view (a) and bottom view (b). (d) Conidiomata formed in culture. (c, e) Conidiophores and conidiogenous cells giving rise to conidia. (f, g) Conidia developed from conidiogenous cells. (h, i) Conidia. Scale bars: 10 μm (e–i).

    • Figure 4. 

      Apiospora baotingensis (CGMCC 3.29222, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i) Conidia. Scale bars: 10 μm. (e–i).

    • Figure 5. 

      Apiospora yinggelingensis (CGMCC 3.29235, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–j) Conidia developed from conidiogenous cells. (k–n) Conidia. Scale bars: 10 μm (e–n).

    • Figure 6. 

      Apiospora campsidis (CGMCC 3.29234, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and top view (c). (d) Conidiomata formed in culture. (e–g, i) Conidiophores and conidiogenous cells giving rise to conidia. (h) Conidia developed from conidiogenous cells. (j) Conidia. (k) Hyphae and conidia. Scale bars: 10 μm (e–k).

    • Figure 7. 

      Apiospora changjiangensis (CGMCC 3.29214, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 8. 

      Apiospora clavata (CGMCC 3.29208, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–g) Conidia developed from conidiogenous cells. (h) Conidia developed from conidiophores. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 9. 

      Apiospora danzhouensis (CGMCC 3.29204, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–i) Conidia developed from conidiogenous cells. (j, k) Conidia. Scale bars: 10 μm (e–k).

    • Figure 10. 

      Apiospora diaoluoshanensis (CGMCC 3.29211, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e) Conidiophores and conidiogenous cells giving rise to conidia. (f, g) Conidia developed from conidiogenous cells. (h) Conidia. Scale bars: 10 μm (e–h).

    • Figure 11. 

      Apiospora dryopteris (CGMCC 3.29219, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days from above (b) and below (c). (d) Conidiomata formed in culture. (e–i) Conidia developed from conidiophores and conidiogenous cells. Scale bars: 10 μm (e–i).

    • Figure 12. 

      Apiospora elongata (CGMCC 3.29205, ex-type culture). (a) Leaf of host plant. (b, c) Colonies after incubation on PDA for 7 days from above (b) and below (c). (d) Conidiomata formed in culture. (e, f) Conidia developed from conidiogenous cells. (g, h) Conidiophores, conidiogenous cells with conidia. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 13. 

      Apiospora guangzhouensis (CGMCC 3.29201, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e, g, h) Conidiophores with conidiogenous cells and conidia. (f) Conidia masses developed from conidiogenous cells. (i) Conidiophores with conidia. j–k Conidia. Scale bars: 10 μm (e–k).

    • Figure 14. 

      Apiospora pseudosasae (CGMCC 3.29202, ex-type culture). a Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e, f) Conidia developed from conidiogenous cells. (g, h) Conidiophores with conidiogenous cells. (i–k) Conidia. Scale bars: 10 μm (e–k).

    • Figure 15. 

      Apiospora lingshuiensis (CGMCC 3.29203, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e, f, i, j) Conidia developed from conidiogenous cells. (g, h) Conidiophores and conidiogenous cells with conidia. (k) Conidia. Scale bars: 10 μm (e–k).

    • Figure 16. 

      Apiospora multiplex (CGMCC 3.29210, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–g) Conidiogenous cells giving rise to conidia. (h) Conidia developed from conidiogenous cells. (i) Hyphae and conidia. (j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 17. 

      Apiospora pingtangensis (CGMCC 3.29217, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidiophores and conidiogenous cells giving rise to conidia. (i) Conidia developed from conidiogenous cells. (j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 18. 

      Apiospora qiongzhongensis (CGMCC 3.29226, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; (b) top view and (c) bottom view. (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 19. 

      Apiospora renhuaensis (CGMCC 3.29221, ex-type culture). (a, b) Colonies after incubation on PDA for 7 days; (a) top view and (b) bottom view. (c) Conidiomata formed in culture. (d–g) Conidiogenous cells with conidia and sterile cells. (h, i) Conidia. (j, k) Sterile cells. Scale bars: 10 μm (d–k).

    • Figure 20. 

      Apiospora rutila (CGMCC 3.29223, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidiophore with conidium. (i, j) Conidia developed from conidiogenous cells. (k, l) Conidia. Scale bars: 10 μm (e–l).

    • Figure 21. 

      Apiospora shaoguanensis (CGMCC 3.29220, ex-type culture). (a, b) Colonies after incubation on PDA for 7 days; top view (a) and bottom view (b). (c) Conidiomata formed in culture. (d) Conidiophores and conidiogenous cells giving rise to conidia. (e, f) Conidia developed from conidiogenous cells. (g, h) Conidia. Scale bars: 10 μm (d–h).

    • Figure 22. 

      Apiospora taianensis (CGMCC 3.29370, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; (b) top view and (c) bottom view. (d) Conidiomata formed in culture. (e, f, h) Conidia developed from conidiogenous cells. (g) Conidia produced from conidiophores and conidiogenous cells. (i) Conidia. (j) Conidia, conidia masses, sterile cells and hyphae. Scale bars: 10 μm (e–j).

    • Figure 23. 

      Apiospora teapae (CGMCC 3.29228, ex-type culture). (a, b) Colonies after incubation on PDA for 7 days; (a) top view and (b) bottom view. (c) Conidiomata formed in culture. (d, e) Conidia developed from conidiophores. (f, g) Conidia developed from conidiogenous cells. (h, i) Conidia. Scale bars: 10 μm (d–i).

    • Figure 24. 

      Apiospora ventricosa (CGMCC 3.29215, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days from above (b) and below (c). (d) Conidiomata formed in culture. (e, f) Conidia developed from conidiophores and conidiogenous cells. (g, h) Conidia developed from conidiogenous cells. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 25. 

      Apiospora wuyishanensis (CGMCC 3.29230, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e, g) Conidia developed from conidiogenous cells. (f) Conidia developed from conidiophores and conidiogenous cells. (h, i) Conidia. Scale bars: 10 μm (e–i).

    • Figure 26. 

      Apiospora zingiberis (CGMCC 3.29227, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i) Conidia developed from conidiophores and conidiogenous cells. (j, k) Conidia. Scale bars: 10 μm (e–k).

    • Figure 27. 

      Phylogenetic tree of the genus Nigrospora based on a concatenated ITS, tef1-α, and tub2 sequences alignment, with Apiospora pseudoparenchymatica serving as the outgroup. The Bayesian inference posterior probability (right, BIPP ≥ 0.90) and the maximum likelihood bootstrap value (left, MLBV ≥ 75%) are shown as MLBV/BIPP above the nodes. Strains marked "*" in the tree are ex-type or ex-epitype. Strains isolated are highlighted in red. The scale bar at the bottom indicates 0.1 substitutions per site. To enhance the visual appeal of the evolutionary tree layout, certain branches are shortened by two diagonal lines ( "//" ) with the number of times.

    • Figure 28. 

      Nigrospora buddlejae (CGMCC 3.29368, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–g) Conidia developed from conidiogenous cells. (h, i) Conidia. Scale bars: 10 μm (e–i).

    • Figure 29. 

      Nigrospora helwingiae (CGMCC 3.29236, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i) Conidia. (j) Hyphae. Scale bars: 10 μm (e–j).

    • Figure 30. 

      Nigrospora officinalis (CGMCC 3.29239, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–g) Conidia developed from conidiogenous cells. (h, i) Conidia. Scale bars: 10 μm (e–i).

    • Figure 31. 

      Nigrospora penniseti (CGMCC 3.29237, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 32. 

      Nigrospora pseudosasae (CGMCC 3.29238, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–h) Conidia developed from conidiogenous cells. (i, j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 33. 

      Phylogenetic tree of the genus Beltraniella based on a concatenated alignment of ITS and LSU sequences, with Castanediella couratarii serving as the outgroup. The Bayesian inference posterior probability (right, BIPP ≥ 0.90) and the maximum likelihood bootstrap value (left, MLBV ≥ 70%) are shown as MLBV/BIPP above the nodes. Strains marked "*" in the tree are represented as ex-type or ex-epitype. Strains in this study are highlighted in red. The scale bar at the bottom indicates 0.01 substitutions per site.

    • Figure 34. 

      Beltraniella obovata (CGMCC 3.29382, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 14 days; (b) top view and (c) bottom view. (d) Conidiophores and setae on pine needle. (e) Asci. (f) Ascospores. (g) Seta. (h) Conidia. Scale bars: 10 μm (e–h).

    • Figure 35. 

      Phylogenetic tree of the genus Neopestalotiopsis based on a concatenated alignment of ITS, tef1-α, and tub2 sequences, with Pestalotiopsis colombiensis and P. diversiseta serving as outgroups. The Bayesian inference posterior probability (right, BIPP ≥ 0.90) and the maximum Likelihood bootstrap value (left, MLBV ≥ 50%) are shown as MLBV/BIPP above the nodes. Strains marked "*" in the tree are represented as ex-type or ex-epitype. Strains in this study are highlighted in red. The scale bar at the bottom indicates 0.1 substitutions per site. To enhance the visual appeal of the evolutionary tree layout, certain branches are shortened by two diagonal lines ( "//" ) with the number of times. The figure shows partial branches of the evolutionary tree and the full evolutionary tree can be found in Supplementary Information 12.

    • Figure 36. 

      Neopestalotiopsis bambooensis (CGMCC 3.29364, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e–f) Conidia developed from conidiogenous cells. (g–j) Conidia. Scale bars: 10 μm (e–j).

    • Figure 37. 

      Phylogenetic tree of the genus Pestalotiopsis based on a concatenated alignment of ITS, tef1-α, and tub2 sequences, with Nonappendiculata quercina serving as the outgroup. The Bayesian inference posterior probability (right, BIPP ≥ 0.90) and the maximum likelihood bootstrap value (left, MLBV ≥ 60%) are shown as MLBV/BIPP above the nodes. Ex-type cultures are indicated in boldface and strains from the present study are highlighted in red. The scale bar at the bottom indicates 0.09 substitutions per site. To enhance the visual appeal of the evolutionary tree layout, certain branches are shortened by four diagonal lines ( "//" ) with the number of times. Furthermore, three single-gene trees were evaluated for Pestalotiopsis (Supplementary Information 13).

    • Figure 38. 

      Pestalotiopsis castanopsis (CGMCC 3.29376, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and top view (c). (d) Conidiomata formed in culture. (e) Conidia developed from conidiogenous cells. (f–n) Conidia. Scale bars: 10 μm (e–n).

    • Figure 39. 

      Pestalotiopsis nanpingensis (CGMCC 3.29375, ex-type culture). (a–b) Colonies after incubation on PDA for 7 days; (a) top view and (b) bottom view. (c) Conidiomata formed in culture. (d) Conidia developed from conidiogenous cells. (e–l) Conidia. Scale bars: 10 μm (d–l).

    • Figure 40. 

      Pestalotiopsis rhizomaticola (CGMCC 3.29378, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e) Conidia developed from conidiogenous cells. (f–n) Conidia. Scale bars: 10 μm (e–n).

    • Figure 41. 

      Pestalotiopsis rostrata (CGMCC 3.29374, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e, f) Conidia developed from conidiogenous cells. (g–k) Conidia. Scale bars: 10 μm (e–k).

    • Figure 42. 

      Pestalotiopsis yuxiensis (CGMCC 3.29373, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e, f) Conidia developed from conidiogenous cells. (g–o) Conidia. Scale bars: 10 μm (e–o).

    • Figure 43. 

      Phylogenetic tree of the genus Pseudopestalotiopsis based on a concatenated ITS, tef1-α, and tub2 sequences alignment, with Neopestalotiopsis clavispora serving as outgroup. The Bayesian Inference Posterior Probability (right, BIPP ≥ 0.90) and the Maximum Likelihood Bootstrap Value (left, MLBV ≥ 95%) are shown as BIPP/MLBV above the nodes. Those marked "*" in the tree are represented as ex-type or ex-epitype strains. Strains isolated in this study were highlighted in red. The scale bar at the bottom indicates 0.01 substitutions per site. To enhance the visual appeal of the evolutionary tree layout, certain branches are shortened by two diagonal lines ( "//" ) with the number of times.

    • Figure 44. 

      Pseudopestalotiopsis xishuangbannaensis (CGMCC 3.29371, ex-type culture). (a) Leaf of the host plant. (b, c) Colonies after incubation on PDA for 7 days; top view (b) and bottom view (c). (d) Conidiomata formed in culture. (e) Conidia developed from conidiogenous cells. (f–k) Conidia. Scale bars: 10 μm (e–k).

    • Loci PCR primers Sequence (5′→3′) PCR cycles Ref.
      ITS ITS5 GGA AGT AAA AGT CGT AAC AAG G 95 °C 5 min; (95 °C 30 s, 55 °C 30 s, 72 °C 1 min) × 35 cycles [47]
      ITS4 TCC TCC GCT TAT TGA TAT GC
      LSU LR0R GTA CCC GCT GAA CTT AAG C 95 °C 5 min; (95 °C: 30 s, 52 °C: 30 s, 72 °C: 1 min) × 35 cycles [48,49]
      LR5 TCC TGA GGG AAA CTT CG
      tef1-α EF1-728F CAT CGA GAA GTT CGA GAA GG 95 °C 5 min; (95 °C 30 s, 55 °C 60 s, 72 °C 1 min) × 30 cycles [50,51]
      EF2 GGA RGT ACC AGT SAT CAT GTT
      tub2 Bt-2a GGT AAC CAA ATC GGT GCT GCT TTC 95 °C 5 min; (95 °C: 30 s, 53 °C: 30 s, 72 °C: 1 min) × 35 cycles [52]
      Bt-2b ACC CTC AGT GTA GTG ACC CTT GGC

      Table 1. 

      Molecular markers, and all PCR primers and programs used in this study.

    • Phylum: Ascomycota
      Subphylum: Pezizomycotina
      Class: Sordariomycetes O.E. Erikss. & Winka
      Order: Amphisphaeriales D. Hawksw. & O.E. Erikss.
      Family: Apiosporaceae K.D. Hyde, J. Fröhl., Joanne E. Taylor & M.E. Barr
      Genus: Apiospora Sacc.
      1. Apiospora adinandrae X.Y. Liu, Z.X. Zhang and X.G. Zhang, Journal of Fungi 10 (1, no. 74): 6 (2024) (new host record)
      2. Apiospora alpiniae Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      3. Apiospora arecacearum L.S. Dissan., K.D. Hyde & J.C. Kang, Mycosphere 15 (1): 1675–1793 (2024) (new host and geographical records)
      4. Apiospora babylonica H. Sheng, Z.X. Zhang & X.G. Zhang, Microorganisms 12: 1372 (2024) (new host and geographical records)
      5. Apiospora baihualingensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      6. Apiospora bambusicaulis K.M. Yu & Y.L. Jiang, MycoKeys 112: 233–252 (2025) (new geographical record)
      7. Apiospora baotingensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      8. Apiospora yinggelingensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      9. Apiospora campsidis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      10. Apiospora changjiangensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      11. Apiospora chiangraiense X.G. Tian & Tibpromma S, Life 11: 1071 (2021) (new host and geographical records)
      12. Apiospora clavata Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      13. Apiospora danzhouensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      14. Apiospora dematiacea C.F. Liao & Doilom, Journal of Fungi 9 (no. 1087): 10 (2023) (new host and geographical records)
      15. Apiospora diaoluoshanensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      16. Apiospora dryopteris Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      17. Apiospora elongata Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      18. Apiospora guangdongensis J.Y. Zhang & Y.Z. Lu, Journal of Fungi 9 (no. 1096): 12 (2023) (new geographical record)
      19. Apiospora guangzhouensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      20. Apiospora hainanensis R.Y. Liu, J.W. Xia & X.G. Zhang, MycoKeys 95: 37 (2023)
      21. Apiospora hydei (Crous) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 207 (2021)
      22. Apiospora hysterina (Sacc.) Pintos & Alvarado, Fungal Systematics and Evolution 7:206P. (2021)
      23. Apiospora intestini (Kajale, Sonawane & Rohit Sharma) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 206 (2021) (new host and geographical records)
      24. Apiospora pseudosasae Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      25. Apiospora jiangxiensis (Mei Wang & L. Cai) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 206 (2021) (new geographical record)
      26. Apiospora lageniformis S.L. Kwon & J.J. Kim, Mycobiology 50 (5): 302–316 (2022) (new host and geographical records)
      27. Apiospora armeniaca H. Sheng, Z.X. Zhang & X.G. Zhang, Microorganisms 2024, 12, 1372 (new host and geographical records)
      28. Apiospora lingshuiensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      29. Apiospora locuta-pollinis (F. Liu & L. Cai) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 207 (2021) (new host record)
      30. Apiospora longistroma (D.Q. Dai & K.D. Hyde) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 207 (2021) (new geographical record)
      31. Apiospora multiplex Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      32. Apiospora pingtangensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      33. Apiospora pseudoparenchymatica (M. Wang & L. Cai) Pintos & P. Alvarado, Fungal Systematics and Evolution 7: 207 (2021) (new geographical record)
      34. Apiospora cyclobalanopsidis (Yao Feng & Jian K. Liu) X.G. Tian & Tibpromma, in Tian, Karunarathna, Mapook, Promputtha, Xu, Bao & Tibpromma, Life 11 (no. 1071): 17 (2021) (new host and geographical records)
      35. Apiospora qiongzhongensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      36. Apiospora renhuaensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      37. Apiospora rutila Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      38. Apiospora shaoguanensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      39. Apiospora taianensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      40. Apiospora teapae Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      41. Apiospora ventricosa Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      42. Apiospora wuyishanensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      43. Apiospora xiangxiensis X.Y. Chang & M.J. Chen, MycoKeys 116: 214 (2025) (new host and geographical records)
      44. Apiospora zingiberis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      Genus: Nigrospora Zimm.
      45. Nigrospora bambusae Mei Wang & L. Cai, Persoonia 39: 127 (2017) (new geographical record)
      46. Nigrospora brasiliensis A.C.Q. Brito, C. Conforto, A.R. Machado, Persoonia 42: 439 (2019) (new host and geographical records)
      47. Nigrospora buddlejae Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      48. Nigrospora camelliae-sinensis Mei Wang & L. Cai, Persoonia 39: 127 (2017) (new host record)
      49. Nigrospora chinensis Mei Wang & L. Cai, Persoonia 39: 129 (2017) (new host record)
      50. Nigrospora helwingiae Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      51. Nigrospora lacticolonia Mei Wang & L. Cai, Persoonia 39: 06 (2017) (new host record)
      52. Nigrospora officinalis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      53. Nigrospora oryzae (Berk. & Broome) Petch (1924) (new host record)
      54. Nigrospora penniseti Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      55. Nigrospora pseudosasae Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      56. Nigrospora pyriformis Mei Wang & L. Cai, Persoonia 39: 126 (2017) (new host record)
      57. Nigrospora rubi Mei Wang & L. Cai, Persoonia 39: 127 (2017) (new host and geographical records)
      58. Nigrospora sacchari-officinarum M. Raza & L. Cai, Fungal Diversity 99: 95 (2019) (new host record)
      59. Nigrospora vesicularifera M. Raza & L. Cai, Fungal Diversity 99: 96 (2019) (new host record)
      Family: Beltraniaceae Nann.
      Genus: Beltraniella Subram.
      60. Beltraniella obovata D.H. Li, J.W. Xia & X.G. Zhang, sp. nov.
      Family: Sporocadaceae Corda
      Genus: Neopestalotiopsis Maharachch., K.D. Hyde & Crous
      61. Neopestalotiopsis bambooensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      62. Neopestalotiopsis mianyangensis W.L. Li & Jian K. Liu, Journal of Fungi 8: 10 (2022) (new host record)
      Genus: Pestalotiopsis Steyaert
      63. Pestalotiopsis castanopsis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      64. Pestalotiopsis dracaenae Yong Wang bis, Y. Song, K. Geng & K.D. Hyde, Fungal Diversity 75: 164 (2015) (new host record)
      65. Pestalotiopsis hydei Huanraluek & Jayaward, Phytotaxa 479 (1): 023–043 (2021) (new host and geographical records)
      66. Pestalotiopsis nanpingensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      67. Pestalotiopsis rhizomaticola Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      68. Pestalotiopsis rostrata Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      69. Pestalotiopsis smilacicola Y.R. Sun & Yong Wang bis, Microbiology Spectrum 11(1): e03987-22, 15 (2023) (new host and geographical records)
      70. Pestalotiopsis yuxiensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.
      Genus: Pseudopestalotiopsis Maharachch., K.D. Hyde & Crous
      71. Pseudopestalotiopsis xishuangbannaensis Q.Y. Liu, Z. Li & X.G. Zhang, sp. nov.

      Table 2. 

      Content of fungal species in this study.