Figures (2)  Tables (2)

    • Figure 1. 

      Phylogram of RA × ML analysis based on the combined LSU and mtSSU sequence datasets. Bootstrap support values for Maximum Likelihood equal to or greater than 70%, and Bayesian posterior probabilities equal to or greater than 0.90 BYPP are indicated at the nodes as ML/BYPP. The newly generated strain in this study is indicated in blue bold text. The tree is rooted with Arthonia didyma (Ertz 7587 BR), A. dispersa (UPSC2583), Cryptothecia candida (Ertz 9260 BR), and Briancoppinsia cytospora (Ertz 15244 BR).

    • Figure 2. 

      Lichenostigma chlaroterae (HKAS148603). (a)–(h) Conidiomata on Lecanora sp. host. (i) Vertical section through conidioma. (j)–(r) Conidia. (s) Development of conidia. Scale bar: (i) = 20 μm, (j)–(r) = 10 μm.

    • Locus Primers PCR: thermal cycles Ref.
      LSU LR5/LR0R (95 ºC: 15 s, 55 ºC: 15 s: 72 ºC: 15 s) × 40 cycles [8]
      mtSSU mrSSU1/
      mrSSU3R      		 (95 ºC: 15 s, 55 ºC: 15 s: 72 ºC: 15 s) × 40 cycles [9]

      Table 1. 

      Genes/loci used in this study with respective PCR primers and protocols.

    • Taxa Strain Genbank accession number
      LSU mtSSU
      Antarctolichenia onofrii CCFEE 6564 MZ005697 –
      Antarctolichenia onofrii CCFEE 6574 MZ005692 –
      Arthonia didyma Ertz 7587 (BR) EU704083 EU704047
      Arthonia dispersa UPSC2583 AY571381 AY571383
      Briancoppinsia cytospora Ertz 15244 (BR) JF830770 JF830772
      Cryptothecia candida Ertz 9260 (BR) HQ454520 EU704052
      Etayoa trypethelii Common 9200-G KF176940 KF176967
      Etayoa trypethelii Common 9215-P KF176941 KF176968
      Etayoa trypethelii Common 9434-K KF176942 KF176969
      Etayoa trypethelii Mukherjee s.n. (BR) KF176943 KF176970
      Lichenostigma alpinum Ertz 17522 (BR) KF176945 KF176972
      Lichenostigma alpinum Diederich 17379 KF176944 KF176971
      Lichenostigma alpinum Ertz 17519 (BR) KF176946 KF176973
      Lichenostigma chlaroterae Neuberg (h Diederich) KF176948 KF176975
      Lichenostigma chlaroterae Goward 12-30 – KF176976
      Lichenostigma chlaroterae Goward 12-42 KF176949 KF176977
      Lichenostigma chlaroterae HKAS 148603 PX885106 PX896563
      Lichenostigma maureri Diederich 17326 (h Diederich) KF176953 KF176979
      Lichenostigma maureri Diederich 17337 KF176952 KF176980
      Lichenostigma maureri Diederich 17306 KF176951 KF176978
      Lichenostigma sp. Ertz 16122 (BR) KF176956 KF176982
      Lichenostigma sp. Ertz 16340 (BR) KF176957 KF176983
      Lichenostigma sp. Ertz 16455 (BR) KF176958 KF176984
      Lichenostigma sp. Ertz 17457 (BR) KF176959 KF176985
      Lichenostigma sp. Ertz 17540 (BR) KF176960 KF176986
      Lichenostigmatales sp. LMCC0526 MH259005 MH259138
      Lichenostigmatales sp. LMCC0528 MH259007 –
      Lichenostigmatales sp. LMCC0529 MH259008 –
      Lichenostigmatales sp. LMCC0530 MH259009 –
      Phaeococcomyces nigricans CBS 553.90 MH873917 –
      Phaeococcomyces sp. G506 OP600268 OP600250
      Phaeococcomyces sp. G148 OP600267 OP600249
      Newly generated sequences are in bold font and the unavailable sequences are indicated by '–'.

      Table 2. 

      Taxa used in the phylogenetic analyses and their GenBank accession numbers.