[1]

De Morais MG, Vaz BDS, De Morais EG, Costa JAV. 2015. Biologically Active Metabolites Synthesized by Microalgae. Biomed Research International 2015:835761

doi: 10.1155/2015/835761
[2]

Draaisma RB, Wijffels RH, Slegers PME, Brentner LB, Roy A, et al. 2013. Food commodities from microalgae. Current Opinion in Biotechnology 24(2):169−77

doi: 10.1016/j.copbio.2012.09.012
[3]

Matos J, Cardoso C, Bandarra NM, Afonso C. 2017. Microalgae as healthy ingredients for functional food: A review. Food & Function 8(8):2672−85

doi: 10.1039/c7fo00409e
[4]

Vigani M, Parisi C, Rodríguez-Cerezo E, Barbosa MJ, Sijtsma L, et al. 2015. Food and feed products from micro-algae: Market opportunities and challenges for the EU. Trends in Food Science & Technology 42(1):81−92

doi: 10.1016/j.jpgs.2014.12.004
[5]

Laurens LML, Van Wychen S, McAllister JP, Arrowsmith S, Dempster TA, et al. 2014. Strain, biochemistry, and cultivation-dependent measurement variability of algal biomass composition. Analytical Biochemistry 452(1):86−95

doi: 10.1016/j.ab.2014.02.009
[6]

Batista AP, Gouveia L, Bandarra NM, Franco JM, Raymundo A. 2013. Comparison of microalgal biomass profiles as novel functional ingredient for food products. Algal Research 2(2):164−73

doi: 10.1016/j.algal.2013.01.004
[7]

Caporgno MP, Mathys A. 2018. Trends in microalgae incorporation into innovative food products with potential health benefits. Frontiers in Nutrition 5:58

doi: 10.3389/fnut.2018.00058
[8]

Bernaerts TMM, Gheysen L, Foubert I, Hendrickx ME, Van Loey AM. 2019. The potential of microalgae and their biopolymers as structuring ingredients in food: a review. Biotechnology Advances 37:107419

doi: 10.1016/j.biotechadv.2019.107419
[9]

Van Durme J, Goiris K, De Winne A, De Cooman L, Muylaert K. 2013. Evaluation of the volatile composition and sensory properties of five species of microalgae. Journal of Agricultural and Food Chemistry 61(46):10881−90

doi: 10.1021/jf403112k
[10]

Spolaore P, Joannis-Cassan C, Duran E, Isambert A. 2006. Commercial applications of microalgae. Journal of Bioscience and Bioengineering 101(2):87−96

doi: 10.1263/jbb.101.87
[11]

Ferdous UT, Yusof ZNB. 2021. Medicinal prospects of antioxidants from algal sources in cancer therapy. Frontiers in Pharmacology 12:593116

doi: 10.3389/fphar.2021.593116
[12]

García-Segovia P, Pagán-Moreno MJ, Lara IF, Martínez-Monzó J. 2017. Effect of microalgae incorporation on physicochemical and textural properties in wheat bread formulation. Food Science and Technology International 23(5):437−47

doi: 10.1177/1082013217700259
[13]

Rao A, Briskey D, Nalley JO, Ganuza E. 2020. Omega-3 eicosapentaenoic acid (EPA) rich extract from the microalga Nannochloropsis decreases cholesterol in healthy individuals: A double-blind, randomized, placebo-controlled, three-month supplementation study. Nutrients 12(6):1869

doi: 10.3390/nu12061869
[14]

Schwenzfeier A, Helbig A, Wierenga PA, Gruppen H. 2013. Emulsion properties of algae soluble protein isolate from Tetraselmis sp. Food Hydrocolloids 30(1):258−63

doi: 10.1016/j.foodhyd.2012.06.002
[15]

Magpusao J, Giteru S, Oey I, Kebede B. 2021. Effect of high pressure homogenization on microstructural and rheological properties of A. platensis, Isochrysis, Nannochloropsis and Tetraselmis species. Algal Research 56:102327

doi: 10.1016/j.algal.2021.102327
[16]

AOAC. 2008. Solids (total) and loss on drying (moisture) in flour: Air oven method (AOAC 925.10-1925). In Official Methods of Analysis of AOAC International 665(1925). Oxford: Oxford University Press. https://doi.org/10.1093/9780197610145.003.2927

[17]

Burja AM, Armenta RE, Radianingtyas H, Barrow CJ. 2007. Evaluation of fatty acid extraction methods for Thraustochytrium sp. ONC-T18. Journal of Agricultural and Food Chemistry 55(12):4795−801

doi: 10.1021/jf070412s
[18]

AOAC. 2005. Protein (total) in flour (AOAC 920.87-1920). In Official Methods of Analysis of AOAC International 4007. Oxford: Oxford University Press. https://doi.org/10.1093/9780197610145.003.2942

[19]

González López CV, García MdCC, Fernández FGA, Bustus CS, Chisti Y, et al. 2010. Protein measurements of microalgal and cyanobacterial biomass. Bioresource Technology 101:7587−91

doi: 10.1016/j.biortech.2010.04.077
[20]

Lourenço SO, Barbarino E, Lavín PL, Lanfer Marquez UM, Aidar E. 2004. Distribution of intracellular nitrogen in marine microalgae: Calculation of new nitrogen-to-protein conversion factors. European Journal of Phycology 39(1):17−32

doi: 10.1080/0967026032000157156
[21]

Kulkarni S, Nikolov Z. 2018. Process for selective extraction of pigments and functional proteins from Chlorella vulgaris. Algal Research 35:185−93

doi: 10.1016/j.algal.2018.08.024
[22]

Wellburn AR. 1994. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. Journal of Plant Physiology 144(3):307−13

doi: 10.1016/S0176-1617(11)81192-2
[23]

Li HB, Cheng KW, Wong CC, Fan KW, Chen F, et al. 2007. Evaluation of antioxidant capacity and total phenolic content of different fractions of selected microalgae. Food Chemistry 102(3):771−76

doi: 10.1016/j.foodchem.2006.06.022
[24]

Bernaerts TMM, Panozzo A, Doumen V, Foubert I, Gheysen L, et al. 2017. Microalgal biomass as a (multi)functional ingredient in food products: Rheological properties of microalgal suspensions as affected by mechanical and thermal processing. Algal Research 25:452−63

doi: 10.1016/j.algal.2017.05.014
[25]

Khrisanapant P, Kebede B, Leong SY, Oey I. 2019. A comprehensive characterisation of volatile and fatty acid profiles of legume seeds. Foods 8(12):651

doi: 10.3390/foods8120651
[26]

Kebede BT, Grauwet T, Magpusao J, Palmers S, Michiels C, Hendrickx M, et al. 2015. An integrated fingerprinting and kinetic approach to accelerated shelf-life testing of chemical changes in thermally treated carrot puree. Food Chemistry 179:94−102

doi: 10.1016/j.foodchem.2015.01.074
[27]

Ryckebosch E, Bruneel C, Muylaert K, Foubert I. 2012. Microalgae as an alternative source of omega-3 long chain polyunsaturated fatty acids. Lipid Technology 24(6):128−30

doi: 10.1002/lite.201200197
[28]

Tokuşoglu Ö, Ünal MK. 2003. Biomass nutrient profiles of three microalgae: Spirulina platensis, Chlorella vulgaris, and Isochrisis galbana. Journal of Food Science 68(4):1144−48

doi: 10.1111/j.1365-2621.2003.tb09615.x
[29]

Graziani G, Schiavo S, Nicolai MA, Buono S, Fogliano V, et al. 2013. Microalgae as human food: Chemical and nutritional characteristics of the thermo-acidophilic microalga Galdieria sulphuraria. Food & Function 4(1):144−52

doi: 10.1039/c2fo30198a
[30]

Rebolloso-Fuentes MM, Navarro-Pérez A, García-Camacho F, Ramos-Miras JJ, Guil-Guerrero JL. 2001. Biomass Nutrient Profiles of the Microalga Nannochloropsis. Journal of Agricultural and Food Chemistry 49(6):2966−72

doi: 10.1021/jf0010376
[31]

Bernaerts TMM, Gheysen L, Kyomugasho C, Jamsazzadeh Kermani Z, Vandionant S, et al. 2018. Comparison of microalgal biomasses as functional food ingredients: Focus on the composition of cell wall related polysaccharides. Algal Research 32:150−61

doi: 10.1016/j.algal.2018.03.017
[32]

Grossmann L, Ebert S, Hinrichs J, Weiss J. 2018. Production of protein-rich extracts from disrupted microalgae cells: Impact of solvent treatment and lyophilization. Algal Research 36:67−76

doi: 10.1016/j.algal.2018.09.011
[33]

Das D. 2015. Algal Biorefinery: An Integrated Approach. New Delhi, India: Springer International Publishing, Jointly published with Capital Publishing Company. 479 pp. https://doi.org/10.1007/978-3-319-22813-6

[34]

Saoudi-Helis L, Dubacq JP, Marty Y, Samain JF, Gudin C. 1994. Influence of growth rate on pigment and lipid composition of the microalga Isochrysis aff. galbana clone T. iso. Journal of Applied Phycology 6(3):315−22

doi: 10.1007/BF02181945
[35]

Gheysen L, Bernaerts T, Bruneel C, Goiris K, Durme J Van. 2018. Impact of processing on n-3 LC-PUFA in model systems enriched with microalgae. Food Chemistry 268(June):441−50

doi: 10.1016/j.foodchem.2018.06.112
[36]

Guo W, Zhu S, Feng G, Wu L, Feng Y, et al. 2020. Microalgae aqueous extracts exert intestinal protective effects in Caco-2 cells and dextran sodium sulphate-induced mouse colitis. Food & Function 11(1):1098−109

doi: 10.1039/c9fo01028a
[37]

Abd El Baky HH, El Baroty GS, Ibrahem EA. 2015. Functional characters evaluation of biscuits sublimated with pure phycocyanin isolated from Spirulina and Spirulina biomass. Nutricion Hospitalaria 32(1):231−41

doi: 10.3305/nh.2015.32.1.8804
[38]

Di Lena G, Casini I, Lucarini M, Lombardi-Boccia G. 2019. Carotenoid profiling of five microalgae species from large-scale production. Food Research International 120:810−18

doi: 10.1016/j.foodres.2018.11.043
[39]

Parniakov O, Apicella E, Koubaa M, Barba FJ, Grimi N, et al. 2015. Ultrasound-assisted green solvent extraction of high-added value compounds from microalgae Nannochloropsis spp. Bioresource Technology 198:262−67

doi: 10.1016/j.biortech.2015.09.020
[40]

Matos J, Cardoso CL, Falé P, Afonso CM, Bandarra NM. 2020. Investigation of nutraceutical potential of the microalgae Chlorella vulgaris and Arthrospira platensis. International Journal of Food Science & Technology 55(1):303−12

doi: 10.1111/ijfs.14278
[41]

Custódio L, Soares F, Pereira H, Barreira L, Vizetto-Duarte C, et al. 2014. Fatty acid composition and biological activities of Isochrysis galbana T-ISO, Tetraselmis sp. and Scenedesmus sp.: Possible application in the pharmaceutical and functional food industries. Journal of Applied Phycology 26(1):151−61

doi: 10.1007/s10811-013-0098-0
[42]

Günerken E, D'Hondt E, Eppink MHM, Garcia-Gonzalez L, Elst K, et al. 2015. Cell disruption for microalgae biorefineries. Biotechnology Advances 33(2):243−60

doi: 10.1016/j.biotechadv.2015.01.008
[43]

Sili C, Torzillo G, Vonshak A. 2012. Arthrospira (Spirulina). In Ecology of Cyanobacteria II: Their Diversity in Space and Time, ed. Whitton BA. Dordrecht: Springer. pp. 677–705. https://doi.org/10.1007/978-94-007-3855-3_25

[44]

Liu C, Lin L. 2001. Ultrastructural study and lipid formation of Isochrysis sp. Botanical Bulletin of Academia Sinica 42:207−14

[45]

Scholz MJ, Weiss TL, Jinkerson RE, Jing J, Roth R, et al. 2014. Ultrastructure and composition of the Nannochloropsis gaditana cell wall. Eukaryotic Cell 13(11):1450−64

doi: 10.1128/EC.00183-14
[46]

Halim R, Rupasinghe TWT, Tull DL, Webley PA. 2013. Mechanical cell disruption for lipid extraction from microalgal biomass. Bioresource Technology 140:53−63

doi: 10.1016/j.biortech.2013.04.067
[47]

Björn A, La Monja PSde, Karlsson A, Ejlertsson J, Svensson BH. 2012. Rheological Characterization. In Biogas, ed. Kumar S. Sweden: InTech. pp. 63−76. https://doi.org/10.5772/32596

[48]

Shekarabi SPH, Mehrgan MS, Razi N, Sabzi S. 2019. Biochemical composition and fatty acid profile of the marine microalga Isochrysis galbana dried with different methods. Journal of Microbiology, Biotechnology and Food Sciences 9(3):521−24

doi: 10.15414/jmbfs.2019/20.9.3.521-524
[49]

Gu N, Lin Q, Li G, Tan Y, Huang L, et al. 2012. Effect of salinity on growth, biochemical composition, and lipid productivity of Nannochloropsis oculata CS 179. Engineering in Life Sciences 12(6):631−37

doi: 10.1002/elsc.201100204
[50]

Milovanović I, Mišan A, Simeunović J, Kovač D, Jambrec D, et al. 2015. Determination of volatile organic compounds in selected strains of cyanobacteria. Journal of Chemistry 2015:969542

doi: 10.1155/2015/969542
[51]

Nawar WW. 1996. Lipids. In Food Chemistry, ed. Fennema OR. 3rd Edition. New York, USA: Marcel Dekker Inc. pp. 225–314.

[52]

Achyuthan KE, Harper JC, Manginell RP, Moorman MW. 2017. Volatile metabolites emission by in vivo microalgae—an overlooked opportunity? Metabolites 7(3):39

doi: 10.3390/metabo7030039
[53]

Isleten Hosoglu M. 2018. Aroma characterization of five microalgae species using solid-phase microextraction and gas chromatography – mass spectrometry/olfactometry. Food Chemistry 240:1210−18

doi: 10.1016/j.foodchem.2017.08.052
[54]

Kettlitz B, Scholz G, Theurillat V, Cselovszky J, Buck NR, et al. 2019. Furan and methylfurans in foods: an update on occurrence, mitigation, and risk assessment. Comprehensive Reviews in Food Science and Food Safety 18(3):738−52

doi: 10.1111/1541-4337.12433
[55]

Zhou L, Chen J, Xu J, Li Y, Zhou C, et al. 2017. Change of volatile components in six microalgae with different growth phases. Journal of the Science of Food and Agriculture 97(3):761−69

doi: 10.1002/jsfa.7794
[56]

Bernaerts TMM, Verstreken H, Dejonghe C, Gheysen L, Foubert I, et a. 2020. Cell disruption of Nannochloropsis sp. improves in vitro bioaccessibility of carotenoids and ω3-LC-PUFA. Journal of Functional Foods 65:103770

doi: 10.1016/j.jff.2019.103770
[57]

Di Lena G, Casini I, Lucarini M, Sanchez del Pulgar J, Aguzzi A, et al. 2020. Chemical characterization and nutritional evaluation of microalgal biomass from large-scale production: a comparative study of five species. European Food Research and Technology 246(2):323−32

doi: 10.1007/s00217-019-03346-5
[58]

Buono S, Langellotti AL, Martello A, Rinna F, Fogliano V. 2014. Functional ingredients from microalgae. Food & Function 5:1669−85

doi: 10.1039/c4fo00125g
[59]

Yamamoto M, Baldermann S, Yoshikawa K, Fujita A, Mase N, et al. 2014. Determination of volatile compounds in four commercial samples of Japanese Green Algae using solid phase microextraction gas chromatography mass spectrometry. The Scientific World Journal 2014:289780

doi: 10.1155/2014/289780
[60]

National Institute of Standards and Technology (NIST). 2019. NIST Chemistry WebBook: NIST Standard Reference Database Number 69. https://doi.org/10.18434/T4D303