Morphological and molecular characterization of culturable leaf endophytic fungi from Malabar Spinach: The first report
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Department of Cell Biology and Molecular Genetics, Sri Devaraj Urs Academy of Higher Education and Research, Kolar – 563 103, Karnataka, India
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2.
Department of Plant Pathology, Gandhi Krishi Vignana Kendra, University of Agricultural Sciences, Bangalore 560 065, India
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Received:
12 July 2019
Revised:
01 September 2019
Published online:
09 October 2019
Studies in Fungi
4(1): 192−204(2019) |
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Abstract
To isolate and identify the cultivable leaf endophytic fungi of Basella alba L. and B. rubra L., healthy and fresh leaves were chosen and collected from Sri Devaraj Urs Academy of Higher Education and Research college botanical garden. Authenticated materials were made into small bits, surface sterilized by applying the standard protocol and cultured on a potato dextrose agar plate supplemented with appropriate antibiotics, incubated for 7 to10 days at 28℃. Seed borne fungal endophytes were also identified by keeping scarified seeds on sterilized agarose. A phylogenetic tree was constructed for the fungi of both the plants. A total of 26 and 19 endophytic cultivable fungi were isolated from B. alba and B. rubra respectively. Majority of the endophytes belongs to the genus Alternaria and several genera in class Dothideomycetes. Our preliminary observation indicated that the vertically transmitted endophytic fungi are the dominant endophytes of the selected plants. We conclude that Alternaria is predominant in both the plants. The mechanism of transition of the microbe from being non-pathogenic in its host to being pathogenic in other plants needs to be studied and elucidated.
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Copyright: © 2019 by the author(s). This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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S Moutusi, P Buela Parivallal, MK Prasannakumar, P Kiranmayee. 2019. Morphological and molecular characterization of culturable leaf endophytic fungi from Malabar Spinach: The first report. Studies in Fungi 4(1):192−204 doi: 10.5943/sif/4/1/21
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S Moutusi, P Buela Parivallal, MK Prasannakumar, P Kiranmayee. 2019. Morphological and molecular characterization of culturable leaf endophytic fungi from Malabar Spinach: The first report. Studies in Fungi 4(1):192−204 doi: 10.5943/sif/4/1/21
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