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Carboxylesterase activity of filamentous soil fungi from a potato plantation in Mankayan, Benguet

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  • In this study, filamentous fungi were isolated from a soil sample from a farm in Mankayan, Benguet. The isolates were tested for the presence of carboxylesterase enzyme as it would indicate the ability to breakdown pyrethroid pesticides such as Cypermethrin. A total of fourteen fungal isolates were characterized morphologically and were identified using the D1/D2 regions of 28S rDNA. All were identified to be members of the Ascomycetes. Seven of the isolates belong to the genus Fusarium, and two were identified to be Aspergillus heteromorphus and Penicillium sp. All fourteen isolates exhibited carboxylesterase activity. Isolates BDP3 and BDP10 exhibited the greatest carboxylesterase activity. These two isolates, both unidentified Ascomycetes, are promising species for mycoremediation specifically targeting pyrethroid pesticides.
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    M Poncian, BJW Beray, HCP Dadulla, RM Hipol. 2019. Carboxylesterase activity of filamentous soil fungi from a potato plantation in Mankayan, Benguet. Studies in Fungi 4(1):292−303 doi: 10.5943/sif/4/1/31
    M Poncian, BJW Beray, HCP Dadulla, RM Hipol. 2019. Carboxylesterase activity of filamentous soil fungi from a potato plantation in Mankayan, Benguet. Studies in Fungi 4(1):292−303 doi: 10.5943/sif/4/1/31

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ARTICLE   Open Access    

Carboxylesterase activity of filamentous soil fungi from a potato plantation in Mankayan, Benguet

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Studies in Fungi  4 Article number: 31  (2019)  |  Cite this article

Abstract: In this study, filamentous fungi were isolated from a soil sample from a farm in Mankayan, Benguet. The isolates were tested for the presence of carboxylesterase enzyme as it would indicate the ability to breakdown pyrethroid pesticides such as Cypermethrin. A total of fourteen fungal isolates were characterized morphologically and were identified using the D1/D2 regions of 28S rDNA. All were identified to be members of the Ascomycetes. Seven of the isolates belong to the genus Fusarium, and two were identified to be Aspergillus heteromorphus and Penicillium sp. All fourteen isolates exhibited carboxylesterase activity. Isolates BDP3 and BDP10 exhibited the greatest carboxylesterase activity. These two isolates, both unidentified Ascomycetes, are promising species for mycoremediation specifically targeting pyrethroid pesticides.

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    Cite this article
    M Poncian, BJW Beray, HCP Dadulla, RM Hipol. 2019. Carboxylesterase activity of filamentous soil fungi from a potato plantation in Mankayan, Benguet. Studies in Fungi 4(1):292−303 doi: 10.5943/sif/4/1/31
    M Poncian, BJW Beray, HCP Dadulla, RM Hipol. 2019. Carboxylesterase activity of filamentous soil fungi from a potato plantation in Mankayan, Benguet. Studies in Fungi 4(1):292−303 doi: 10.5943/sif/4/1/31
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