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2020 Volume 5
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ARTICLE   Open Access    

Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides

  • 1.

    Saltillo Experimental Station, Instituto Nacional de Investigaciones Forestales Agrí

  • 2.

    Department of Agricultural Parasitology, Universidad Autó

  • 3.

    Food Research Department, School of Chemical Sciences, Universidad Autónoma de Coahuila, 25000, Saltillo, Coahuila, Mexico

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  • The aims of this study are to determine the population genetic structure of Rhizoctonia solani, associated with potato, and to assess its resistance degree to pencycuron and thiabendazole fungicides. A total of 57 strains of R. Solani were isolated from different potato crop fields in Mexico, and were classified according to their anastomosis group (AG) using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP)-based analysis. Then, the amplified fragments were digested with four different endonucleases (Mse I, Ava II, Mun I and Hinc II). The Repetitive Element Sequence-Based PCR (rep-PCR) technique was used to determine the genetic diversity in R. solani populations. Results obtained by PCR-RFLP showed that 81% of the isolates were identified as belonging to AG-3 group, 14% to AG1-1B and 5% to AG-11 group. In response to fungicides inhibition, IC50 between 0.014-0.039 mg a.i./L for pencycuron, and 0.82 to 2.91 for thiabendazole were determined, This IC50 value showed that the resistance factor (RF) values ranged from 1.4 to 3.945 and 0.27 to 0.97, respectively; this tendency suggests that isolates are susceptible to thiabendazole. The AG groups showed a heterogeneous resistance to pencycuron and thiabendazole, being more tolerant to these fungicides AG1-1B and AG-11 groups. The genetic analysis shows a low genetic diversity among (P-value 0.3225) and within (P-value 0.3275) populations; this can indicate a clonal reproduction and little parasexual cycle among the analyzed isolations.
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    RF Castillo, G Gallegos, OA Flores, GC Aguilar, HR Rodriguez, CFD Hernandez. 2020. Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides. Studies in Fungi 5(1):381−391 doi: 10.5943/sif/5/1/19
    RF Castillo, G Gallegos, OA Flores, GC Aguilar, HR Rodriguez, CFD Hernandez. 2020. Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides. Studies in Fungi 5(1):381−391 doi: 10.5943/sif/5/1/19
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ARTICLE   Open Access    

Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides

  • 1.

    Saltillo Experimental Station, Instituto Nacional de Investigaciones Forestales Agrí

  • 2.

    Department of Agricultural Parasitology, Universidad Autó

  • 3.

    Food Research Department, School of Chemical Sciences, Universidad Autónoma de Coahuila, 25000, Saltillo, Coahuila, Mexico

  • Received: 19 March 2020
  • Revised: 17 June 2020
  • Published online: 21 June 2020
Studies in Fungi  5,  Article number: 19  (2020)  |  Cite this article

Abstract: The aims of this study are to determine the population genetic structure of Rhizoctonia solani, associated with potato, and to assess its resistance degree to pencycuron and thiabendazole fungicides. A total of 57 strains of R. Solani were isolated from different potato crop fields in Mexico, and were classified according to their anastomosis group (AG) using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP)-based analysis. Then, the amplified fragments were digested with four different endonucleases (Mse I, Ava II, Mun I and Hinc II). The Repetitive Element Sequence-Based PCR (rep-PCR) technique was used to determine the genetic diversity in R. solani populations. Results obtained by PCR-RFLP showed that 81% of the isolates were identified as belonging to AG-3 group, 14% to AG1-1B and 5% to AG-11 group. In response to fungicides inhibition, IC50 between 0.014-0.039 mg a.i./L for pencycuron, and 0.82 to 2.91 for thiabendazole were determined, This IC50 value showed that the resistance factor (RF) values ranged from 1.4 to 3.945 and 0.27 to 0.97, respectively; this tendency suggests that isolates are susceptible to thiabendazole. The AG groups showed a heterogeneous resistance to pencycuron and thiabendazole, being more tolerant to these fungicides AG1-1B and AG-11 groups. The genetic analysis shows a low genetic diversity among (P-value 0.3225) and within (P-value 0.3275) populations; this can indicate a clonal reproduction and little parasexual cycle among the analyzed isolations.

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    Cite this article
    RF Castillo, G Gallegos, OA Flores, GC Aguilar, HR Rodriguez, CFD Hernandez. 2020. Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides. Studies in Fungi 5(1):381−391 doi: 10.5943/sif/5/1/19
    RF Castillo, G Gallegos, OA Flores, GC Aguilar, HR Rodriguez, CFD Hernandez. 2020. Genetic variability of Rhizoctonia solani Kühn and its resistance to fungicides. Studies in Fungi 5(1):381−391 doi: 10.5943/sif/5/1/19
    shu

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