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Figure 1.  Phylogenetic analysis of the YABBY transcript factors of C. sinensis. Cs, Camellia sinensis; Vv, Vitis vinefera; At, Arabidopsis thaliana, Br, Brassicarapa, Bs, Bienertia sinuspersici; Cq, Chenopodium quinoa. Protein information of YABBYs in the phylogenetic tree is shown in Supplemental Table S2. The phylogenetic tree was constructed by the p-distance method using MEGA X.

Figure 2.  Motifs of the YABBY family proteins from C. sinensis, V. vinefera, A. thaliana, B. rapa, B. sinuspersici, and C. quinoa. The motifs were identified using MEME Suite 5.1.0 server on line (http://meme-suite.org/tools/meme). The detailed protein information is listed in Supplemental Table S2.

Figure 3.  Relative expression level of CsYABBY genes in different tissues, organs, or callus of tea plants. S1−S3, the different developmental stages of flowers. The data for (a) is derived from TPIA previously established by our laboratory^[22].

Figure 4.  Expression levels of CsYABBY genes in different tissues or organs of tea plants by quantitative PCR analysis. S1−S6, the different developmental stages of flowers. 1st−4th leaf, the different developmental leaf of tea plants.

Figure 5.  The curl phenotypes of leaves caused by overexpression of CsFILa, CsFILb and CsYAB2 in Arabidopsis. (a) Phenotypes of leaves of Arabidopsis overexpressed of CsFILa, CsFILb and CsYAB2 (T4 generation). (b) The rolling index of leaves at different developmental stage from the transgenic Arabidopsis. The leaf rolling index (LRI) was calculated according to the formula: LRI = (Lw − Ln) / Lw × 100%^[21]. Significant differences are represented by asterisks. P-value less than 0.05 and 0.01 indicate statistical significance and extreme significance, respectively (n = 3, * P < 0.05, ** P < 0.01).

Figure 6.  Leaf development and CsYABBYs expression pattern in leaves of the picked and unpicked shoots. (a) The development of leaf on the picked or unpicked shoots within 48 h. (b) Variation of width and length of leaf on the unpicked shoots within 48 h. (c) Variation of width and length of leaf on the picked shoots within 48 h after picking. (d) The expression pattern of CsFILa, CsFILb and CsYAB2 in tea leaves on the unpicked shoots. (e) The expression pattern of CsFILa, CsFILb and CsYAB2 in tea leaves on the picked shoots.

Figure 7.  Leaf development and CsYABBYs expression pattern in leaves of the picked shoots treated with 10% and 15% PEG. (a) The developmental phenotype of the leaf on the picked shoots treated with 10% and 15% PEG within 48 h. (b) Variation of width and length of the leaf on the picked shoots treated with 10% PEG within 48 h. (c) Variation of width and length of the leaf on the picked shoots treated with 15% PEG within 48 h. (d) The expression level of CsFILa, CsFILb and CsYAB2 in the leaf on the picked shoots treated with 10% PEG. (e) The expression level of CsFILa, CsFILb and CsYAB2 in the leaf on the picked shoots 15% PEG.