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Figure 1. 

Alternaria alternata strains AAU-CS-F4, AAU-CS-F9 and AAU-CS-F10 inciting flower blight on Camellia sinensis. (a) Healthy flower bud; (b) natural flower blight appearing on tea plants taken in the field; (c, d) colony on PDA for 7 d (front and reverse); (e) short chains of conidia of dwarf dendrites; (f) conidia; (g) chlamydospore. Scale bars = 20 μm.

Figure 2. 

Phylogenetic analysis of AAU-CS-F4, AAU-CS-F9 and AAU-CS-F10. Phylogenetic tree was inferred from the combined ITS, GAPDH and TUB sequences of the genus Alternaria. The phylogenetic tree was constructed using the maximum likelihood method (1,000 replicates) by MEGA 7.0. The isolates in this study are marked with a red star. Scale bars represent 0.01 nucleotide substitutions per locus.

Figure 3. 

Symptoms observed on the flowers in C. sinensis plant grown at 3 d post-inoculation. (a, b) Three day sterile water group and (c, d) spore inoculation group.

Figure 4. 

A. alternata strains inciting inconspicuous leaf disease on C. sinensis at 10 d post-inoculation. Symptoms observed on a leaf in C. sinensis grown as the sterile water group and spore inoculation group after 0 d (a, c) and 10 d (b, d).