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Figure 1.
Diagram of the final step in the agar well diffusion method.
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Figure 2.
Diagram of the final step in the poison food method.
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Figure 3.
Effect of different concentrations of (a) A. sativum, (b) A. vera, (c) A. indica and (d) Z. Officinale aqueous extractions against all five pathogens after 7 d of incubation.
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Figure 4.
Effect of different concentrations of plant extracts against the selected seed-borne pathogens; A. niger, O. foliicola, and T. oumae-annae. (a) A. niger in negative controls; a1-Methanol 80%, a2-n-hexane, a3-DMSO 50%; (b) A. niger in A. vera (b1-10%, b2-15%, b3-20% concentrations); (c) A. niger in A. sativum (c1-10%, c2-15%, c3-20% concentrations); (d) A. niger in Z. officinale (d1-10%, d2-15%, d3-20% concentrations); (e) A. niger in Captan 50 % (WP) positive control (e1, e2, e3-Captan 50 % (WP) positive control) ; (f) O. foliicola in negative controls; f1-Methanol 80%, f2-n-hexane, f3-DMSO 50%; (g) O. foliicola in A. vera (g1-10%, g2-15%, g3-20% concentrations); (h) O. foliicola in A. sativum (h1-10%, h2-15%, h3-20% concentrations); (i) O. foliicola in A. indica (i1-10%, i2-15%, i3-20% concentrations); (j) O. foliicola in Z. officinale (j1-10%, j2-15%, j3-20% concentrations); (k) T. oumae-annae in negative controls; k1-Methanol 80%, k2-n-hexane, k3-DMSO 50%; (l) T. oumae-annae in A. vera (l1-10%, l2-15%, l3-20% concentrations); (m) T. oumae-annae in A. sativum (m1-10%, m2-15%, m3-20% concentrations); (n) T. oumae-annae in Z. officinale (n1-10%, n2-15%, n3-20% concentrations); (o) T. oumae-annae Captan 50 % (WP) positive control (o1, o2, o3-Captan 50 % (WP) positive control).
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Figure 5.
Effect of different concentrations of (a) A. sativum, (b) A. vera, (c) A. indica and (d) Z. Officinale aqueous extractions against all five pathogens after 7 d of incubation.
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Figure 6.
Effect of different concentrations of Z. officinale extract against the selected seed-borne pathogen; A. niger and A. flavus. (a) A. niger in negative control (distilled water); (b) A. niger at 20% concentration; (c) A. niger in crude extract; (d) A. niger in Captan 50 % positive control; (e) A. flavus in negative control (distilled water); (f) A. flavus in 20% concentration; (g) A. flavus in crude extract (h) A. flavus in Captan 50 % (WP) positive control.
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Figure 7.
Seed germination data of Arachis hypogea treated with (a) Azadirachta indica, (b) Aloe vera, (c) Allium sativum, and V. sinensis treated with (d) Allium sativum, (e) Aloe vera and (f) Zingiber officinale aqueous extractions.
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Plant extract Seed
varietyStorage period
(months)% Germination % Abnormal
seedlingSpeed of
germination indexVigor index Allium sativum Arachis hypogea > 6 80 20 2.17 1912.8 Aloe vera > 6 60 − 0.94 2118 Azadirachta indica > 6 70 − 2.20 1710.8 Zingiber officinale > 6 50 10 1.02 1205 Distilled water > 6 30 30 0.71 713.1 Captan (50%) > 6 80 − 2.31 3329.6 Allium sativum Oryza sativa > 6 70 20 1.33 2170 Aloe vera > 6 80 − 1.66 1565.6 Azadirachta indica > 6 100 − 4.42 2824 Zingiber officinale > 6 100 30 4.72 3079 Distilled water > 6 80 − 1.73 1742 Captan (50%) > 6 100 − 4.73 4385 Allium sativum Vigna radiata > 6 100 − 5.42 2404 Aloe vera > 6 95 − 5.02 2011.2 Azadirachta indica > 6 100 10 5.42 2269 Zingiber officinale > 6 100 − 5.42 2664 Distilled water > 6 100 30 5.40 2306 Captan (50%) > 6 100 20 5.42 2238 Allium sativum Vigna sinensis > 6 20 − 1.08 509.4 Aloe vera > 6 15 − 0.81 396.4 Azadirachta indica > 6 30 − 1.42 686.4 Zingiber officinale > 6 25 − 0.96 627 Distilled water > 6 5 − 0.23 105 Captan (50%) > 6 30 10 1.09 705 Table 1.
Effect of different plant extracts on seed germination data of Arachis hypogea, Oryza sativa, Vigna radiata, V. sinensis seeds.
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Plant extract (w/v) Storage period
(months)% Germination % Abnormal
seedlingSpeed of
germination indexVigor index Allium sativum 10
15
20> 6 20
35
55− 1.08
1.95
2.20705
1092.4
1564.8Aloe vera 10
15
20> 6 10
30
20− 0.42
0.94
0.94273
822.3
470Azadirachta indica 10
15
20> 6 20
30
20− 1.35
1.62
1.77670
904.8
250Zingiber officinale 10
15
20> 6 15
15
30− 1.95
2.32
2.32397.5
449.8
983.4Distilled water − > 6 5 − 0.23 105 n-Hexane − > 6 4 − 1.11 89.0 Methanol (80%) − > 6 5 − 0.94 84.6 DMSO (50%) − > 6 4 − 0.87 77.0 Captan (50%) − > 6 30 10 1.09 705 Table 2.
Effect of different plant extracts on seed germination data of Vigna sinensis seeds.
Figures
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Tables
(2)