Figures (6)  Tables (3)
    • Figure 1. 

      Phenotypic differences in leaves of 33 Dichondra repens genotypes under normal conditions.

    • Figure 2. 

      Differences in (a) plant height, (b) leaf area, (c) leaf thickness, and (d) petiole length among 33 Dichondra repens genotypes under normal conditions. Vertical bars represent standard errors of the mean (n = 10).

    • Figure 3. 

      Cluster analysis of 33 Dichondra repens genotypes based on SSR markers.

    • Figure 4. 

      Differences in drought stress index of (a) relative water content (RWC) and (b) electrolyte leakage (EL) among 33 Dichondra repens genotypes. Vertical bars represent standard errors of the mean (n = 4).

    • Figure 5. 

      Differences in drought stress index of (a) chlorophyll (Chl), (b) photosystem II photochemical efficiency (Fv/Fm), and (c) performance index on absorption basis (PIABS) among 33 Dichondra repens genotypes. Vertical bars represent standard errors of the mean (n = 4).

    • Figure 6. 

      Changes in (a) heat map and (b) principal component analysis (PCA) based on five different physiological parameters. RWC, relative water content; EL, electrolyte leakage; Chl, chlorophyll; Fv/Fm, photosystem II photochemical efficiency; PIABS, performance index on absorption basis.

    • Primer
      name
      Total number of
      amplified bands
      Number of
      polymorphic bands
      PPB (%)PIC
      C241818100.000.338
      C271919100.000.295
      C3017952.940.163
      C331313100.000.314
      C51111090.910.354
      C601818100.000.193
      C661313100.000.342
      C6788100.000.230
      C71231982.610.230
      Z25211362.900.163
      Z3733100.000.266
      Z57191684.210.266
      Z699888.890.215
      Z1131616100.000.292
      Z1351111100.000.279
      SSR111212100.000.305
      IBM1344100.000.111
      IBM445211885.710.292
      Total256228
      Average14.2212.6791.590.258

      Table 1. 

      Amplification results among 33 Dichondra repens using different SSR primers.

    • Material No.RWCELChlFv/FmPIABSAverageOrder
      Dr50.8360.6410.9320.8790.6420.7861
      Dr330.7580.8550.7190.8490.4740.7312
      Dr30.6520.6180.8980.9040.4550.7063
      Dr40.6810.6120.8470.8680.4620.6944
      Dr320.6420.7060.6370.8230.4610.6545
      Dr20.5750.6130.7220.8390.4330.6366
      Dr10.5360.5720.7980.8420.3910.6287
      Dr180.8240.5770.7520.6580.3120.6258
      Dr310.5950.7660.6960.7420.2950.6199
      Dr90.4580.3280.9170.8510.4440.60010
      Dr60.6860.4340.5700.7640.4120.57311
      Dr250.5920.5450.7450.7230.2530.57212
      Dr170.7350.3140.7080.7270.3610.56913
      Dr160.4980.4400.7360.7570.2960.54514
      Dr220.6560.5690.5100.6520.2520.52815
      Dr190.5390.3960.7030.6430.3060.51716
      Dr100.6670.4520.7030.5920.1730.51717
      Dr120.5120.4300.6720.6710.2480.50618
      Dr230.5230.4530.5450.6790.2740.49519
      Dr210.6080.6170.4280.5730.2080.48720
      Dr240.5580.4220.4550.6340.2720.46821
      Dr70.7170.3710.5790.5390.1060.46222
      Dr150.4850.4440.5410.6050.2070.45623
      Dr300.5370.4840.3630.6470.2430.45524
      Dr260.4250.4550.5230.6350.2300.45325
      Dr130.4310.3910.7180.5470.1630.45026
      Dr110.5120.4830.6520.4600.1420.45027
      Dr200.6760.2900.1880.7000.2550.42228
      Dr80.3620.4120.5670.3290.1440.36329
      Dr140.4060.2810.6200.2780.1450.34630
      Dr280.5050.2710.2720.5140.1560.34431
      Dr270.3230.2950.3210.2940.1130.26932
      Dr290.0000.0000.0000.0000.0000.00033

      Table 2. 

      Membership function values of five physiological parameters and comprehensive evaluation of drought tolerance among 33 Dichondra repens genotypes.

    • Material No.OriginAltitude (m)
      Dr1Zhongjiang, Sichuan600
      Dr2Pingtang, Guizhou848
      Dr3Dushan, Guizhou1010
      Dr4Tianzhu, Guizhou350
      Dr5Naxi, Sichuan404
      Dr6Dayi, Sichuan310
      Dr7Bishan, Chongqing350
      Dr8Jining, Yunnan1890
      Dr9Xifeng,Guizhou990
      Dr10Xishui, Guizhou1169
      Dr11Sinan, Guizhou730
      Dr12Jiangkou, Guizhou475
      Dr13Tongren, Guizhou415
      Dr14Zhenyuan, Guizhou382
      Dr15Danzai, Guizhou894
      Dr16Sandu, Guizhou500
      Dr17Sandu, Guizhou780
      Dr18Dujun, Guizhou842
      Dr19Shuicheng, Guizhou1193
      Dr20Liuzhi, Guizhou1035
      Dr21Anshun, Guizhou1278
      Dr22Qinglong, Guizhou1393
      Dr23Jin’an, Guizhou1336
      Dr24Panzhou, Guizhou1532
      Dr25Xingren, Guizhou1336
      Dr26Anlong, Guizhou1250
      Dr27Wangmo, Guizhou653
      Dr28Ziyun, Guizhou1160
      Dr29Huishui, Guizhou980
      Dr30 (‘Silver Falls’)USA-
      Dr31Xichou, Yunnan1108
      Dr32 (‘Xiaoshao’)Yiliang, Yunnan1970
      Dr33 (‘Duliujiang’)Sandu, Guizhou600

      Table 3. 

      Test 33 Dichondra repens materials and their sources.