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Figure 1.
Phenotypic differences in leaves of 33 Dichondra repens genotypes under normal conditions.
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Figure 2.
Differences in (a) plant height, (b) leaf area, (c) leaf thickness, and (d) petiole length among 33 Dichondra repens genotypes under normal conditions. Vertical bars represent standard errors of the mean (n = 10).
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Figure 3.
Cluster analysis of 33 Dichondra repens genotypes based on SSR markers.
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Figure 4.
Differences in drought stress index of (a) relative water content (RWC) and (b) electrolyte leakage (EL) among 33 Dichondra repens genotypes. Vertical bars represent standard errors of the mean (n = 4).
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Figure 5.
Differences in drought stress index of (a) chlorophyll (Chl), (b) photosystem II photochemical efficiency (Fv/Fm), and (c) performance index on absorption basis (PIABS) among 33 Dichondra repens genotypes. Vertical bars represent standard errors of the mean (n = 4).
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Figure 6.
Changes in (a) heat map and (b) principal component analysis (PCA) based on five different physiological parameters. RWC, relative water content; EL, electrolyte leakage; Chl, chlorophyll; Fv/Fm, photosystem II photochemical efficiency; PIABS, performance index on absorption basis.
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Primer
nameTotal number of
amplified bandsNumber of
polymorphic bandsPPB (%) PIC C24 18 18 100.00 0.338 C27 19 19 100.00 0.295 C30 17 9 52.94 0.163 C33 13 13 100.00 0.314 C51 11 10 90.91 0.354 C60 18 18 100.00 0.193 C66 13 13 100.00 0.342 C67 8 8 100.00 0.230 C71 23 19 82.61 0.230 Z25 21 13 62.90 0.163 Z37 3 3 100.00 0.266 Z57 19 16 84.21 0.266 Z69 9 8 88.89 0.215 Z113 16 16 100.00 0.292 Z135 11 11 100.00 0.279 SSR11 12 12 100.00 0.305 IBM13 4 4 100.00 0.111 IBM445 21 18 85.71 0.292 Total 256 228 — — Average 14.22 12.67 91.59 0.258 Table 1.
Amplification results among 33 Dichondra repens using different SSR primers.
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Material No. RWC EL Chl Fv/Fm PIABS Average Order Dr5 0.836 0.641 0.932 0.879 0.642 0.786 1 Dr33 0.758 0.855 0.719 0.849 0.474 0.731 2 Dr3 0.652 0.618 0.898 0.904 0.455 0.706 3 Dr4 0.681 0.612 0.847 0.868 0.462 0.694 4 Dr32 0.642 0.706 0.637 0.823 0.461 0.654 5 Dr2 0.575 0.613 0.722 0.839 0.433 0.636 6 Dr1 0.536 0.572 0.798 0.842 0.391 0.628 7 Dr18 0.824 0.577 0.752 0.658 0.312 0.625 8 Dr31 0.595 0.766 0.696 0.742 0.295 0.619 9 Dr9 0.458 0.328 0.917 0.851 0.444 0.600 10 Dr6 0.686 0.434 0.570 0.764 0.412 0.573 11 Dr25 0.592 0.545 0.745 0.723 0.253 0.572 12 Dr17 0.735 0.314 0.708 0.727 0.361 0.569 13 Dr16 0.498 0.440 0.736 0.757 0.296 0.545 14 Dr22 0.656 0.569 0.510 0.652 0.252 0.528 15 Dr19 0.539 0.396 0.703 0.643 0.306 0.517 16 Dr10 0.667 0.452 0.703 0.592 0.173 0.517 17 Dr12 0.512 0.430 0.672 0.671 0.248 0.506 18 Dr23 0.523 0.453 0.545 0.679 0.274 0.495 19 Dr21 0.608 0.617 0.428 0.573 0.208 0.487 20 Dr24 0.558 0.422 0.455 0.634 0.272 0.468 21 Dr7 0.717 0.371 0.579 0.539 0.106 0.462 22 Dr15 0.485 0.444 0.541 0.605 0.207 0.456 23 Dr30 0.537 0.484 0.363 0.647 0.243 0.455 24 Dr26 0.425 0.455 0.523 0.635 0.230 0.453 25 Dr13 0.431 0.391 0.718 0.547 0.163 0.450 26 Dr11 0.512 0.483 0.652 0.460 0.142 0.450 27 Dr20 0.676 0.290 0.188 0.700 0.255 0.422 28 Dr8 0.362 0.412 0.567 0.329 0.144 0.363 29 Dr14 0.406 0.281 0.620 0.278 0.145 0.346 30 Dr28 0.505 0.271 0.272 0.514 0.156 0.344 31 Dr27 0.323 0.295 0.321 0.294 0.113 0.269 32 Dr29 0.000 0.000 0.000 0.000 0.000 0.000 33 Table 2.
Membership function values of five physiological parameters and comprehensive evaluation of drought tolerance among 33 Dichondra repens genotypes.
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Material No. Origin Altitude (m) Dr1 Zhongjiang, Sichuan 600 Dr2 Pingtang, Guizhou 848 Dr3 Dushan, Guizhou 1010 Dr4 Tianzhu, Guizhou 350 Dr5 Naxi, Sichuan 404 Dr6 Dayi, Sichuan 310 Dr7 Bishan, Chongqing 350 Dr8 Jining, Yunnan 1890 Dr9 Xifeng,Guizhou 990 Dr10 Xishui, Guizhou 1169 Dr11 Sinan, Guizhou 730 Dr12 Jiangkou, Guizhou 475 Dr13 Tongren, Guizhou 415 Dr14 Zhenyuan, Guizhou 382 Dr15 Danzai, Guizhou 894 Dr16 Sandu, Guizhou 500 Dr17 Sandu, Guizhou 780 Dr18 Dujun, Guizhou 842 Dr19 Shuicheng, Guizhou 1193 Dr20 Liuzhi, Guizhou 1035 Dr21 Anshun, Guizhou 1278 Dr22 Qinglong, Guizhou 1393 Dr23 Jin’an, Guizhou 1336 Dr24 Panzhou, Guizhou 1532 Dr25 Xingren, Guizhou 1336 Dr26 Anlong, Guizhou 1250 Dr27 Wangmo, Guizhou 653 Dr28 Ziyun, Guizhou 1160 Dr29 Huishui, Guizhou 980 Dr30 (‘Silver Falls’) USA - Dr31 Xichou, Yunnan 1108 Dr32 (‘Xiaoshao’) Yiliang, Yunnan 1970 Dr33 (‘Duliujiang’) Sandu, Guizhou 600 Table 3.
Test 33 Dichondra repens materials and their sources.
Figures
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Tables
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