Taxonomy and biology of <i>Cladosporium endophyticum</i> as the first record in Iraq

Figures (3) Tables (6)

Figure 1.
Cladosporium endophyticum. (a) Colony; (b)−(e) Conidiophores with conidial chains; (f) Conidiogenous cell; (g) Conidiophore macronematocyst; (h), (i) Conidia. Scale bars (a) = 10 mm, (b)−(i) = 10 m.
Figure 2.
Comparison of nucleic acid sequences. Cladosporium endophyticum For example, 'ref' is a reference sequence, while 'S1' is a sampling number.
Figure 3.
Phylogenetic tree of Cladospoium endophyticum with Cladosporium sphaerospermum and Cladosporium halotolerans. Variable colors represent the variable grouping within the Genbank deposited sequences of the investigated variations. The symbol S1 denotes the code for the sample under investigation in this study.

Amplicon	Reference locus sequences (5′ - 3′)	Length
rRNA	TCCGTAGGTGAACCTGCGGAGGGATCATTACAAGTTGACCCCGGCCCTCGGGCCGGGATGTTCACAACCCTTTGTTGTCCGACTCTGTTGCCTCCGGGGCGACCCTGCCTCCGGGCGGGGGCCCCGGGTGGACACTTCAAACTCTTGCGTAACTTTGCAGTCTGAGTAAATTTAATTAATAAATTAAAACTTTCAACAACGGATCTCTTGGTTCTGGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATGTGAATTGCAGAATTCAGTGAATCATCGAATCTTTGAACGCACATTGCGCCCCCTGGTATTCCGGGGGGCATGCCTGTTCGAGCGTCATTTCACCACTCAAGCCTCGCTTGGTATTGGGCGACGCGGTCCGCCGCGCGCCTCAAATCGACCGGCTGGGTCTTCCGTCCCCTCAGCGTTGTGGAAACTATTCGCTAAAGGGTGCCGCGGGAGGTCACGCCGCAAAACAACCCCATTTCTAAGGTTGACCTCGGATCAGGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGGCGGAGGA	554 bp

Table 1.

Cladosporium endophyticum (NR 158360.1) has a ribosomal subunit (rsu) rRNA that was amplified using a 554 bp PCR amplicon.

Temperature Mean Std. deviation

5 0 0
10 8.66 0.57
15 17.33 1.52
20 38 1
25 46 2
30 28.66 1.15
35 6.33 1.15
40 0 0

RLSD = 1.698 (P ≤ 0.05)

Table 2.
Impact of different temperatures on radial growth of C. endophyticum grown on PDA medium for 14 d.
pH Mean Std. deviation

4 21.66 1.52
5 34 1
6 39.33 0.57
7 28.33 1.52
8 18.33 1.52

RLSD = 2 (P ≤ 0.05)

Table 3.
Impact of different pH values on radial growth of C. endophyticum grown on PDA medium at 25°C for 14 d.
Media Mean Std. deviation

PDA 44.66 1.52
MEA 42 1
OA 32.33 2.08
SNA 30.66 1.52

RLSD = 2.5 (P ≤ 0.05)

Table 4.
Impact of different media on radial growth of C. endophyticum incubated at 25 °C for 14 d.
Carbon source Mean Std. deviation

Sucrose 45.33 0.57
Glucose 44.00 1
Xylose 32.33 1.15
Sorbitol 27.66 0.57
Mannitol 25.00 1

RLSD = 1.39 (P ≤ 0.05)

Table 5.
Impact of various carbon sources on radial growth of C. endophyticum incubator at 25 °C for 14 d.
Nitrogen source Mean Std. deviation

Ammonium nitrate 45.66 1.15
Ammonium phosphate 39.66 0.57
Ammonium sulphate 37.00 2
Urea 32.66 2.08
Potasium nitrate 32.33 0.57

RLSD = 2.3 (P ≤ 0.05)

Table 6.
Impact of various nitrogen sources on radial growth of C. endophyticum at 25 °C for 14 d.