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Figure 1. 

Electrophoresis analyses of 1.0 μM of SSF and DSF. (a) SDS-PAGE analysis. (b) Native-PAGE analysis. Lane 1: SSF; Lane 2: DSF; Lane M: Protein markers.

Figure 2. 

Electrophoresis analyses of 1.0 μM of SSF and DSF after different thermal treatments. (a) SDS-PAGE analysis of SSF. (b) SDS-PAGE analysis of DSF. (c) Native-PAGE analysis of SSF. (d) Native-PAGE analysis of DSF. Lane M: Protein markers.

Figure 3. 

CD spectra results of ferritins under conditions of untreated, 68 °C for 30 min and 100 °C for 10 min. (a) CD spectra and percentages of the secondary structure of 0.1 mg/mL SSF. (b) CD spectra and percentages of the secondary structure of 0.2 mg·mL⁻¹ DSF. Values are means ± SD, and different letters in the column indicate significant difference (P < 0.05) between treatments.

Figure 4. 

Iron content of ferritins after thermal treatment measured by ICP-MS, with untreated as a control. (a) and (b) are the iron content of SSF (1.0 μM) and DSF (1.0 μM) under conditions of untreated, 68 °C for 30 min and 100 °C for 10 min, respectively. Values are means ± SD, and different letters in the column indicate significant differences (P < 0.05) between treatments.

Figure 5. 

In vivo digestion results of SSF and DSF after different treatments. Lanes 1−3: Untreated, 68 °C for 30 min, 100 °C for 10 min of SSF; Lanes 4−6: Untreated, 68 °C for 30 min, 100 °C for 10 min of DSF. Control group: mice administered with 300 μL of 10 mM phosphate buffer. The electrophoresis was performed three times, with similar results obtained.

Figure 6. 

Serum iron content of mice in SSF groups and DSF groups. The letters indicate inner group comparisons of SSF groups (P < 0.05). There is no statistical difference of DSF groups (P > 0.05). Control group: mice administered with 300 μL of 10 mM phosphate buffer. Values are means ± SD, and different letters in the column indicate significant differences (P < 0.05) between treatments.