A study on chilling hardiness of three persimmon genotypes by electrolyte leakage parameter

Figures (6) Tables (4)

Figure 1.
The effect of 'genotype × phenological phase' on proline content.
Figure 2.
The effect of 'genotype × chilling × phenological stage' on proline content.
Figure 3.
The effect of chilling on electrolyte leakage (EL).
Figure 4.
The effect of 'genotype × chilling × phenological phase' on electrolyte leakage (EL).
Figure 5.
The effect of chilling on EC.
Figure 6.
The effect of 'genotype × chilling × phenological stage' on EC.

Sources of variations	df	Means of squares
Sources of variations	df	Proline content	Electrolyte leakage	EC
Replication	2	88.74^**	89.39^ns	32.14^ns
Genotype (A)	2	19.48^ns	71.32^ns	117.79^ns
Chilling (B)	1	21.37^ns	234.04^*	284.97^*
AB	2	29.13^ns	138.85^*	461.82^**
Phenological phase (C)	2	369.23^**	6673.85^**	7819.65^**
AC	4	40.96^*	26.31^*	115.24^ns
BC	2	10.59^ns	143.87^*	285.73^*
ABC	4	65.57^**	85.79⁺	464.13^**
Error	34	14.42	33.59	59.83
ns: insignificant difference; *: significant difference at 0.01; : significant difference at 0.05; +: significant difference at 0.10.

Table 1.

Analysis of variance for the effect of experimental factors on proline content.

Treatment	Proline (μmol/g FW)	Electrolyte leakage (%)	EC (μS/cm)
Bud dormancy	6.52 b	2.54 b	0.1 b
Bud swelling	14.66 a	34.64 a	36.23 a
Anthesis	7.15 b	0.16 b	0.16 b
Similar letters in each column shows insignificant differences at the p < 0.05 level.

Table 2.

Means comparison for the effect of phenological stage on the studied traits.

Treatment	Ion leakage (%)	EC (μS/cm)
4 °C × bud dormancy	1.60 c	0.10 c
4 °C × bud swelling	29.34 b	29.33 b
4 °C × anthesis	0.16 c	0.16 c
-16 °C × bud dormancy	3.48 c	0.11 c
-16 °C × bud swelling	39.94 a	43.13 a
-16 °C × anthesis	0.16 c	0.15 c
Similar letters in each column shows insignificant differences at the p < 0.01 and p < 0.05 levels.

Table 3.

Means comparison for the effect of 'chilling × phenological phase' on the studied traits.

Treatment	Ion leakage (%)	EC (μS/cm)
Date-plum × 4 °C	9.92 b	8.68 b
Date-plum × -16 °C	19.56 a	19.80 a
Kaki Fuyu × 4 °C	12.15 b	12.84 ab
Kaki Fuyu × -16 °C	10.68 b	5.77 b
Japanese × 4 °C	9.02 b	8.07 b
Japanese × -16 °C	13.35 b	17.82 a
Similar letter(s) in each column shows insignificance differences at the p < 0.01 and p < 0.05 levels.

Table 4.

Means comparison for the effect of 'genotype × chilling' on the studied traits.