Floral bud differentiation and mechanism underlying androdioecy of <i>Osmanthus fragrans</i>

Yuanji Han; Yanxia He; Shumin Yue; Bingao Guo; Qian Zhu; Huijun Zhang; Xiangzhan Hai; Fude Shang; Yuanji Han; Yanxia He; Shumin Yue; Bingao Guo; Qian Zhu; Huijun Zhang; Xiangzhan Hai; Fude Shang

doi:10.48130/OPR-2023-0011

Figures (5) Tables (1)

Figure 1.
Floral bud differentiation of sweet osmanthus. (a) − (g) Involucre differentiation. (h), (i) flower primordium differentiation. (j) − (n) Terminal flower perianth differentiation. (o), (p) Stamen differentiation. (q) − (s) Pistil differentiation. (t) − (v) Pistil differentiation (sterile). in: Involucre primordium, sf: Side flower primordium, se: Sepal primordium, pe: Petal primordium, an: Stamen primordium, pi: Pistil primordium.
Figure 2.
Development of the pistil of the two cultivars. (a) The full flowering stage of 'Huangchun Jingui' cultivar. (b) Fruit formation of 'Huangchun Jingui' cultivar. (c) The full flowering stage of 'Chenghong Dangui' cultivar. (d) Pistil abortion and no fruit formation in 'Chenghong Dangui' cultivar.
Figure 3.
Morphological characteristics and activity of pollen grains of the two cultivars. (a) − (h) Morphological characteristics of pollen grains. (i) − (n) Activity of pollen grains of the two cultivars.
Figure 4.
Differential expression of genes related to flower organ development in the two cultivars. (a) Differential expression genes related to flower organ development in the linggeng stage. (b) Differential expression genes related to flower organ development in the xingyan stage. (c) Differential expression genes related to flower organ development in the initial flowering stage. (d) Differential expression genes related to flower organ development in the full flowering stage. (e) Differential expression genes related to flower organ development in the late full flowering stage. Y: 'Huangchuan Jingui', D: 'Chenghong Dangui'. Y1 and D1: Linggeng stage, Y2 and D2: Xingyan stage, Y3 and D3: Initial flowering stage, Y4 and D4: Full flowering stage, Y5 and D5: Late full flowering stage. Three replicates for each analysis.
Figure 5.
Transcriptional regulation of CRC and AG genes. (a) The effector and reporter plasmids used in dual-LUC assays. REN: Renilla luciferase, LUC: Firefly luciferase. (b) The AG promoter activity (LUC/REN ratio) of tobacco leaves coinfiltration with Agrobacteria carrying effector and reporter. (c) The CRC promoter activity (LUC/REN ratio) of tobacco leaves coinfiltration with Agrobacteria carrying effector and reporter. The data represent the means ± SD of three replicates from three independent experiments. * P < 0.05.

Cultivars	Polar axis (P) (μm)	Equator axis (E) (μm)	P/E	Pore groove length (L) (μm)	L/P
'Huangchuan Jingui'	18.0 ± 1.01	10.8 ± 1.05	1.63 ± 0.11	12.4 ± 1.52	0.70 ± 0.08
'Chenghong Dangui'	18.6 ± 0.66*	10.9 ± 0.54	1.70 ± 0.12*	13.9 ± 1.21*	0.77 ± 0.05*
* P < 0.05

Table 1.

Measurement results of pollen.