Characteristic volatile components and transcriptional regulation of seven major tea cultivars (<i>Camellia sinensis</i>) in China

Ting Gao; Shuxian Shao; Binghao Hou; Yaping Hong; Weiwei Ren; Shan Jin; Shuilian Gao; Pengjie Wang; Naixing Ye; Ting Gao; Shuxian Shao; Binghao Hou; Yaping Hong; Weiwei Ren; Shan Jin; Shuilian Gao; Pengjie Wang; Naixing Ye

doi:10.48130/BPR-2023-0017

Figures (8) Tables (2)

Figure 1.
Multivariate statistical analysis of volatile components of seven tea cultivars. (a) Phenotypes of one bud and two leaves and their suitability. (b) PCA principal component analysis. (c) Types and relative contents of volatile components. (d) Proportions of volatile components.
Figure 2.
Analysis of differential volatile components of seven tea cultivars.
Figure 3.
Expression profiles of genes related to the terpene synthesis pathway. (a) Heatmap of the expression pattern of CsTPS genes. (b) Biosynthetic pathway of terpenes and expression patterns of related DEGs. (c) Metabolic pathway of carotenoids and expression patterns of related DEGs.
Figure 4.
Expression profiles of genes related to the α-linolenic acid metabolism pathway.
Figure 5.
Expression profiles of genes related to the phenylpropanoid/benzenoid synthesis pathway.
Figure 6.
Coexpression network related to key aroma compound formation. Matrix of module-metabolite associations. The abscissa represents different phenotypes, and the ordinate represents different modules. The number of genes per module is shown in the left box. Correlation coefficients and p-values between modules and metabolites are shown at the row-column intersection. Red means the module has a greater correlation with the phenotype, and blue means the module has a lower correlation with the phenotype.
Figure 7.
Coexpression network diagram of candidate genes. When the correlation coefficient (r) is greater than 0.8, we believe that there is a regulatory relationship between the candidate genes and TFs. Nodes with the same colour indicate that the correlation coefficient between candidate genes and TFs is greater than 0.8, and the line colours between nodes indicate the strength of the correlation.
Figure 8.
Verification of the expression levels of eight differentially expressed genes.

ID	CAS	RT /min	Compounds	Relative contents (µg/kg)
ID	CAS	RT /min	Compounds	TGY	JX	FJSX	FDDB	BHZ	LJ43	SCZ
1	106-24-1	14.27	Geraniol	1,358.90 ± 49.69^d	5103.36 ± 85.9^a	3,017.74 ± 80.37^b	2,573.91 ± 69.41^c	239.72 ± 10.4^e	1,321.78 ± 27.49^d	2,510.78 ± 62.78^c
2	78-70-6	11.46	Linalool	1,012.66 ± 10.81^d	1,258.39 ± 74.46^bc	1,106.32 ± 71.3^cd	2,465.09 ± 167.61^a	478.07 ± 44.92^e	1,212.96 ± 18.69^bcd	1,408.27 ± 132.44^b
3	40716-66-3	19.76	(E)-Nerolidol	54.73 ± 2.25^c	54.71 ± 0.86^c	72.67 ± 5.77^b	34.06 ± 1.59^d	107.39 ± 4.41^a	67.08 ± 3.28^b	115.07 ± 5.96^a
4	106-25-2	13.75	Nerol	22.42 ± 0.62^e	72.25 ± 1.44^a	49.45 ± 4.07^cd	60.73 ± 1.75^b	11.29 ± 0.31^f	35.87 ± 0.79^d	60.29 ± 3.10^b
5	928-96-1	6.67	(Z)-3-Hexenol	34.90 ± 0.64^d	33.82 ± 1.24^d	79.71 ± 11.02^c	177.94 ± 10.84^b	73.79 ± 9.13^c	170.01 ± 4.20^b	229.24 ± 26.97^a
6	100-51-6	12.50	Benzyl alcohol	0.28 ± 0.02^a	0.16 ± 0.03^cd	0.16 ± 0.03^bcd	0.22 ± 0.03^ab	0.12 ± 0.01^d	0.21 ± 0.00^bc	0.17 ± 0.02^bcd
7	60-12-8	11.69	Phenylethyl alcohol	95.04 ± 6.81^e	114.96 ± 1.21^e	15.49 ± 0.09^f	739.79 ± 17.52^a	414.46 ± 7.66^c	338.25 ± 5.13^d	689.34 ± 36.16^b
8	505-32-8	23.78	Isophytol	10.36 ± 2.57^b	9.28 ± 1.52^b	12.55 ± 2.90^b	7.46 ± 2.43^b	24.32 ± 8.12^a	8.23 ± 3.84^b	14.38 ± 3.96^ab
9	150-86-7	24.63	Phytol	11.05 ± 3.74^ab	9.64 ± 1.83^ab	18.00 ± 6.78^ab	7.29 ± 3.37^b	30.89 ± 18.38^a	6.36 ± 4.45^b	15.20 ± 9.07^ab
10	29171-23-1	24.77	Dehydroisophytol	2.73 ± 0.71^b	1.83 ± 0.39^b	3.36 ± 1.03^ab	1.71 ± 0.63^b	5.53 ± 1.83^a	1.73 ± 0.93^b	2.86 ± 0.96^b
11	143-08-8	12.74	1-Nonanol	10.40 ± 0.33^c	8.33 ± 0.59^cd	6.59 ± 0.56^d	29.29 ± 0.77^a	21.15 ± 1.31^b	31.58 ± 1.08^a	31.27 ± 2.04^a
12	111-70-6	9.03	1-Heptanol	6.53 ± 0.27^d	5.88 ± 0.27^d	3.77 ± 0.51^d	19.03 ± 1.98^a	11.75 ± 1.10^bc	10.68 ± 0.49^c	13.75 ± 2.12^b
13	10482-56-1	13.24	α-Terpineol	26.66 ± 0.24^a	26.81 ± 1.26^a	19.35 ± 1.44^b	24.79 ± 1.22^a	6.21 ± 0.53^e	10.88 ± 0.71^d	14.51 ± 1.87^c
14	10340-23-5	12.43	(Z)-3-Nonen-1-ol	0.97 ± 0.13^d	1.44 ± 0.12^bc	1.22 ± 0.16^cd	2.26 ± 0.09^a	1.27 ± 0.05^cd	2.34 ± 0.17^a	1.75 ± 0.21^b
15	2425-77-6	22.13	2-Hexyl-1-decanol	2.22 ± 0.05^a	1.22 ± 0.11^d	1.85 ± 0.35^bc	1.63 ± 0.08^c	2.05 ± 0.07^ab	1.64 ± 0.02^c	1.91 ± 0.02^bc
16	481-34-5	21.18	α-Cadinol	3.08 ± 0.08^d	11.32 ± 0.47^a	4.30 ± 0.28^c	1.89 ± 0.13^e	5.97 ± 0.16^b	2.18 ± 0.09^e	1.67 ± 0.14^e
17	5944-20-7	13.83	Isogeraniol	5.75 ± 0.21^e	7.19 ± 0.26^e	23.45 ± 2.80^b	16.03 ± 1.19^c	11.98 ± 0.87^d	30.30 ± 0.99^a	14.74 ± 1.49^cd
18	498-00-0	14.09	Vanillyl alcohol	246.47 ± 2.27^b	242.40 ± 4.17^b	251.82 ± 8.47^ab	257.91 ± 7.73^ab	243.94 ± 6.00^b	265.31 ± 4.52^a	244.78 ± 11.91^b
19	15051-81-7	19.43	epi-g-Eudesmol	1.09 ± 0.10^d	0.90 ± 0.09^de	1.13 ± 0.10^cd	0.66 ± 0.06^e	1.76 ± 0.14^ab	1.43 ± 0.13^bc	1.79 ± 0.29^a
20	18675-33-7	15.29	(+)-Neodihydrocarveol	2.54 ± 0.12^a	1.61 ± 0.06^b	0.79 ± 0.04^d	0.49 ± 0.02^e	1.02 ± 0.11^c	0.00 ± 0.00^f	0.00 ± 0.00^f
21	5989-33-3	10.90	(Z)-Linalool oxide (furanoid)	26.59 ± 1.08^e	97.88 ± 5.72^d	43.45 ± 4.25^e	255.98 ± 13.68^a	84.79 ± 6.13^d	202.69 ± 13.96^b	130.23 ± 11.66^c
22	34995-77-2	11.20	(E)-Linalool oxide (furanoid)	81.38 ± 4.35^e	314.5 ± 19.75^c	81.20 ± 5.77^e	666.69 ± 29.84^a	189.5 ± 12.55^d	408.19 ± 31.09^b	299.17 ± 27.17^c
23	39028-58-5	12.84	(E)-Linalool oxide (pyranoid)	15.78 ± 0.36^d	40.13 ± 2.58^b	12.91 ± 0.95^d	69.64 ± 0.99^a	39.31 ± 2.55^b	34.67 ± 1.30^c	36.72 ± 1.82^bc
Alcohols (23)				3,032.53 ± 74.37^e	7,418.01 ± 149.51^a	4,827.26 ± 203.54^c	7,414.48 ± 291.74^a	2,006.27 ± 48.02^f	4,164.36 ± 50.25^d	5,837.9 ± 253.16^b
24	97-53-0	16.12	Eugenol	3.17 ± 0.58^a	0.73 ± 0.01^c	1.82 ± 0.44^b	1.67 ± 0.13^b	0.95 ± 0.05^c	1.94 ± 0.04^b	2.01 ± 0.14^b
25	3228-02-2	15.13	3-Methyl-4-isopropylphenol	5.46 ± 1.38^a	1.03 ± 0.11^c	3.14 ± 0.68^b	1.82 ± 0.07^bc	0.76 ± 0.12^c	0.99 ± 0.15^c	0.87 ± 0.18^c
Phenols (2)				8.63 ± 1.85^a	1.76 ± 0.12^c	4.96 ± 1.11^b	3.49 ± 0.09b^c	1.71 ± 0.14^c	2.93 ± 0.14^c	2.88 ± 0.29^c
26	141-27-5	14.55	Neral	25.34 ± 1.36^c	93.8 ± 3.25^a	60.48 ± 7.92^b	65.70 ± 7.92^b	5.30 ± 0.95^d	37.78 ± 1.06^c	59.69 ± 6.84^b
27	121-33-5	13.69	Vanillin	3.25 ± 0.28^d	0.74 ± 0.11^e	10.16 ± 2.30^a	5.28 ± 0.45^bcd	3.90 ± 0.21^cd	6.27 ± 0.86^b	6.15 ± 0.11^bc
28	34246-54-3	12.67	3-Ethylbenzaldehyde	0.65 ± 0.01^cd	0.62 ± 0.02^de	0.42 ± 0.05^e	0.70 ± 0.07^cd	0.86 ± 0.12^bc	1.01 ± 0.02^ab	1.10 ± 0.11^a
29	6728-26-3	14.46	(E)-2-Hexenal	3.25 ± 0.48^cd	5.99 ± 0.58^a	4.38 ± 0.19^b	4.10 ± 0.31^bc	1.93 ± 0.12^e	3.16 ± 0.37^d	4.01 ± 0.36^bcd
30	100-52-7	8.87	Benzaldehyde	9.82 ± 0.70^ab	8.20 ± 0.37^bc	6.17 ± 1.08^c	8.72 ± 1.21^bc	9.00 ± 1.21^bc	11.07 ± 0.73^ab	12.86 ± 2.30^a
31	122-78-1	11.69	Phenylacetaldehyde	99.41 ± 7.28^e	121.13 ± 1.40^e	18.87 ± 0.40^f	746.69 ± 12.32^a	435.00 ± 6.55^c	356.40 ± 14.09^d	700.08 ± 30.99^b
32	112-31-2	13.40	Decanal	0.59 ± 0.07^b	0.64 ± 0.15^b	0.69 ± 0.07^b	0.82 ± 0.17^b	0.86 ± 0.14^b	1.19 ± 0.12^a	0.81 ± 0.04^b
33	111-71-7	7.67	Heptanal	143.12 ± 4.70^a	2.18 ± 0.24^b	2.16 ± 0.41^b	1.60 ± 0.24^b	1.15 ± 0.19^b	1.09 ± 0.11^b	0.90 ± 0.11^b
34	66-25-1	5.45	Hexanal	7.83 ± 1.19^d	15.44 ± 3.43^bcd	12.48 ± 1.33^cd	18.63 ± 2.71^bc	27.58 ± 6.03^a	29.47 ± 3.54^a	22.46 ± 1.58^ab
35	123-72-8	5.45	Butanal	6.59 ± 1.30^d	14.07 ± 3.66^bcd	11.59 ± 1.53^cd	16.53 ± 1.67^bc	26.64 ± 5.32^a	28.38 ± 3.38^a	21.63 ± 2.66^ab
36	110-62-3	4.83	Valeraldehyde	14.41 ± 1.13^de	20.84 ± 1.88^cd	13.41 ± 2.76^e	24.21 ± 1.66^c	33.52 ± 2.5^ab	31.13 ± 1.22^b	40.15 ± 5.75^a
37	590-86-3	5.03	Isovaleraldehyde	48.88 ± 1.45^a	21.11 ± 1.69^b	33.19 ± 5.46^ab	37.00 ± 5.6^ab	34.38 ± 8.89^ab	45.65 ± 1.09^a	39.15 ± 11.95^a
Aldehydes (12)				363.15 ± 9.42^c	304.75 ± 8.93^c	174.01 ± 3.96^d	929.96 ± 12.47^a	580.11 ± 28.65^b	552.59 ± 8.81^b	908.99 ± 56.08^a
38	459-80-3	16.02	Geranic acid	37.94 ± 9.28^bc	115.49 ± 33.01^a	62.74 ± 23.82^b	20.81 ± 3.51^bc	8.82 ± 2.85^c	19.69 ± 2.49^c	131.88 ± 17.95^a
39	112-05-0	14.48	Nonanoic acid	2.58 ± 0.66^c	4.45 ± 1.22^ab	4.80 ± 0.65^ab	4.39 ± 0.67^ab	5.51 ± 0.47^a	3.51 ± 0.44^bc	4.64 ± 0.40^ab
40	111-14-8	12.69	Heptanoic acid	119.89 ± 0.98^a	101.69 ± 5.30^cd	115.81 ± 5.88^ab	107.27 ± 3.55^bcd	98.31 ± 5.48^d	111.4 ± 2.49^abc	104.84 ± 6.82^bcd
41	109-52-4	14.48	Pentanoic acid	4.92 ± 0.63^b	7.14 ± 1.45^ab	8.07 ± 1.02^a	8.00 ± 1.74^a	8.12 ± 1.10^a	6.55 ± 0.54^ab	7.57 ± 0.45^ab
42	79-09-4	12.05	Propanoic acid	65.13 ± 26.72^a	79.82 ± 37.7^a	89.19 ± 28.57^a	50.37 ± 21.63^a	65.56 ± 23.49^a	64.99 ± 18.33^a	67.71 ± 24.17^a
Acids (5)				230.47 ± 31.08^ab	308.59 ± 71.53^a	280.6 ± 47.71^ab	190.84 ± 20.11^b	186.32 ± 20.63^b	206.13 ± 16.59^b	316.64 ± 32.43^a
43	127-41-3	9.92	α-Ionone	12.05 ± 0.88^cd	23.28 ± 0.54^a	14.7 ± 1.06^bc	17.81 ± 1.66^b	2.60 ± 0.43^e	10.34 ± 1.64^d	14.11 ± 2.26^c
44	79-77-6	18.39	β-Ionone	87.2 ± 1.64^c	90.97 ± 0.81^c	92.93 ± 7.34^c	103.48 ± 5.56^b	111.25 ± 4.28^ab	108.43 ± 2.05^ab	114.18 ± 2.52^a
45	689-67-8	17.79	Geranylacetone	10.67 ± 0.30^bc	12.01 ± 0.49^a	11.15 ± 1.07^abc	10.07 ± 0.57^c	11.79 ± 0.19^ab	10.58 ± 0.05^bc	11.43 ± 0.29^ab
46	502-69-2	23.10	Fitone	6.01 ± 0.21^bc	4.82 ± 0.37^de	6.70 ± 0.61^b	7.05 ± 0.24^b	9.15 ± 0.70^a	4.17 ± 0.21^e	5.51 ± 0.61^cd
47	471-15-8	11.62	3-Thujone	11.55 ± 3.73^a	1.49 ± 1.68^b	6.56 ± 2.92^ab	0.84 ± 0.34^b	4.92 ± 4.11^b	2.18 ± 1.52^b	0.65 ± 0.44^b
48	488-10-8	16.84	Jasmone	6.64 ± 0.35^d	4.79 ± 0.61^e	4.24 ± 0.28^e	5.18 ± 0.26^e	9.53 ± 0.41^b	7.84 ± 0.53^c	12.43 ± 0.39^a
49	23726-93-4	16.53	β-Damascenone	1.01 ± 0.15^e	18.05 ± 0.75^a	14.26 ± 1.60^b	7.58 ± 0.13^cd	1.82 ± 0.24^e	6.11 ± 0.14^d	9.02 ± 0.31^c
Ketones (7)				135.12 ± 4.62^c	155.41 ± 3.29^ab	150.53 ± 12.41^bc	152.02 ± 6.26^ab	151.07 ± 7.13^bc	149.65 ± 0.72^bc	167.33 ± 4.26^a
50	544-76-3	20.41	Hexadecane	3.25 ± 0.34^a	4.04 ± 0.38^a	3.41 ± 0.90^a	4.73 ± 1.33^a	4.82 ± 0.68^a	4.11 ± 0.54^a	4.26 ± 0.56^a
51	629-59-4	16.97	Tetradecane	7.30 ± 00.43^ab	5.87 ± 0.54^b	6.21 ± 0.46^b	7.82 ± 1.40^ab	7.82 ± 0.95^ab	8.41 ± 0.70^a	7.27 ± 0.84^ab
52	3891-99-4	18.03	2,6,10-Trimethyltridecane	6.51 ± 0.12^bc	5.13 ± 0.72^c	6.91 ± 2.37^abc	6.36 ± 0.50^bc	8.50 ± 0.66^ab	8.21 ± 0.16^ab	9.67 ± 1.60^a
Hydrocarbons (3)				17.07 ± 0.59^ab	15.04 ± 1.09^b	16.54 ± 3.61^ab	18.9 ± 2.56^ab	21.14 ± 2.03^a	20.72 ± 1.21^ab	21.2 ± 2.99^a
53	120-72-9	15.05	Indole	0.00 ± 0.00^f	36.36 ± 1.23^b	0.00 ± 0.00^f	11.8 ± 0.78^d	42.41 ± 1.36^a	7.78 ± 0.62^e	24.75 ± 2.46^c
54	91-64-5	17.72	Coumarin	16.81 ± 1.45^a	18.38 ± 0.34^a	17.67 ± 1.54^a	11.5 ± 0.53^b	10.28 ± 0.58^b	11.43 ± 0.48^b	10.50 ± 0.61^b
55	36431-72-8	15.22	Theaspirane	3.92 ± 0.05^c	10.98 ± 0.92^b	4.57 ± 0.49^c	14.31 ± 1.95^a	5.88 ± 0.24^c	9.10 ± 0.19^b	4.23 ± 0.44^c
Heterocyclic compounds (3)				20.73 ± 1.41^f	65.72 ± 0.98^a	22.24 ± 1.79^f	37.61 ± 2.32^d	58.57 ± 0.92^b	28.31 ± 0.70^e	39.48 ± 2.71^c
56	6753-98-6	18.06	α-Humulene	6.58 ± 0.40^a	4.63 ± 0.11^b	1.16 ± 0.04^e	1.19 ± 0.07^e	3.06 ± 0.21^d	0.86 ± 0.04^e	3.68 ± 0.42^c
57	87-44-5	17.43	β-Caryophyllene	14.30 ± 0.46^a	14.97 ± 0.30^a	2.04 ± 0.28^d	4.54 ± 0.73^c	8.49 ± 0.48^b	0.50 ± 0.19^e	1.62 ± 0.26^d
58	502-61-4	18.79	α-Farnesene	28.28 ± 0.62^c	34.79 ± 0.54^b	12.15 ± 1.56^e	14.98 ± 1.35^e	43.86 ± 1.15^a	12.50 ± 0.69^e	24.02 ± 1.91^d
59	123-35-3	9.39	Myrcene	241.44 ± 15.17^c	669.11 ± 7.54^a	374.82 ± 41.18^b	426.14 ± 44.52^b	44.16 ± 6.77^d	230.59 ± 33.5^c	363.94 ± 50.74^b
60	3779-61-1	10.44	(E)-β-Ocimene	107.36 ± 5.92^c	263.91 ± 3.58^a	147.98 ± 15.26^b	174.1 ± 17.53^b	23.92 ± 4.42^d	99.25 ± 13.74^c	155.4 ± 25.28^b
61	7216-56-0	11.95	Allo-ocimene	14.16 ± 1.00^c	37.51 ± 0.65^a	21.17 ± 2.50^b	23.85 ± 2.91^b	2.43 ± 0.34^d	12.92 ± 2.22^c	20.17 ± 2.98^b
62	99-85-4	10.68	γ-Terpinene	11.22 ± 1.12^b	17.20 ± 0.51^a	12.16 ± 0.68^b	13.79 ± 1.33^b	2.42 ± 0.36^d	8.25 ± 1.30^c	11.29 ± 2.04^b
63	5208-59-3	16.78	β-Bourbonene	4.85 ± 0.11^d	4.44 ± 0.06^d	12.08 ± 2.07^b	8.51 ± 1.21^c	17.81 ± 1.93^a	5.83 ± 0.16^cd	3.54 ± 0.47^d
64	29050-33-7	9.91	4-Carene	12.04 ± 0.92^cd	23.33 ± 0.51^a	14.74 ± 1.06^bc	17.81 ± 1.66^b	2.66 ± 0.40^e	10.34 ± 1.64^d	14.13 ± 2.31^c
65	483-76-1	19.10	δ-Cadinene	12.99 ± 0.13^c	93.21 ± 2.13^a	7.78 ± 0.97^de	6.12 ± 0.41^e	9.49 ± 0.47^d	17.99 ± 0.70^b	6.78 ± 0.55^e
66	21391-99-1	19.51	α-Calacorene	10.27 ± 0.10^b	42.73 ± 0.64^a	6.58 ± 0.53^d	4.65 ± 0.21^e	8.12 ± 0.70^c	10.1 ± 0.44^b	5.53 ± 0.59^de
67	483-77-2	19.16	Calamenene	6.57 ± 0.15^c	61.3 ± 1.03^a	4.16 ± 0.49^de	4.66 ± 0.12^de	5.46 ± 0.70^cd	16.02 ± 0.98^b	3.38 ± 0.62^e
68	5989-27-5	12.19	d-Limonene	5.38 ± 0.38^cd	14.19 ± 0.42^a	8.26 ± 0.99^b	8.95 ± 1.46^b	0.94 ± 0.23^e	4.99 ± 0.79^d	7.49 ± 1.14^bc
Terpenoids (13)				475.44 ± 24.58^c	1281.32 ± 15.03^a	625.07 ± 57.13^b	709.29 ± 67.59^b	172.83 ± 17.3^d	430.14 ± 56.04^c	620.98 ± 89.21^b
69	3681-82-1	9.64	(E)-3-Hexen-1-ol acetate	125.08 ± 1.45^d	223.82 ± 15.90^cd	1030.93 ± 119^a	690.15 ± 92.67^b	398.2 ± 79.2^c	981.62 ± 42.12^a	871.21 ± 172.5^ab
70	61444-38-0	16.64	(Z)-3-Hexenyl (Z)-3-hexenoate	10.90 ± 0.08^d	10.16 ± 0.50^d	46.93 ± 2.48^b	48.58 ± 3.24^b	7.03 ± 0.63^d	23.17 ± 1.03^c	54.08 ± 3.55^a
71	31501-11-8	16.57	(E)-hex-3-enyl hexanoate	5.27 ± 0.10^e	23.15 ± 0.86^d	76.15 ± 6.66^a	26.00 ± 2.08^d	22.04 ± 1.86^d	43.61 ± 1.26^b	36.01 ± 2.91^c
72	2497-18-9	9.82	(E)-2-Hexenyl acetate	0.00 ± 0.00^c	1.48 ± 0.15^bc	8.98 ± 1.42^a	2.34 ± 0.40^bc	7.07 ± 1.83^a	0.00 ± 0.00^c	3.23 ± 0.91^b
73	65405-77-8	22.84	(Z)-3-Hexenyl salicylate	0.12 ± 0.01^d	4.60 ± 0.07^a	4.55 ± 0.53^a	2.40 ± 0.32^c	4.28 ± 0.37^b	3.78 ± 0.16^b	2.22 ± 0.21^d
74	41519-23-7	12.99	(Z)-3-Hexenyl isobutyrate	9.20 ± 0.41^d	20.40 ± 1.44^c	83.98 ± 8.54^a	12.03 ± 1.63^cd	12.81 ± 1.82^cd	39.75 ± 1.61^b	34.51 ± 4.53^b
75	53398-85-9	14.82	(Z)-3-Hexenyl 2-methylbutyrate	0.00 ± 0.00^e	0.22 ± 0.02^d	1.23 ± 0.15^a	0.21 ± 0.02^d	0.28 ± 0.02^d	0.87 ± 0.06^b	0.53 ± 0.08^c
76	35852-46-1	13.90	(Z)-3-Hexenyl valerate	0.23 ± 0.05^e	0.61 ± 0.10^e	5.30 ± 0.89^a	1.89 ± 0.32^cd	3.32 ± 0.51^b	2.15 ± 0.05^c	1.05 ± 0.13^de
77	1189-09-9	15.51	Methyl geranate	3.73 ± 0.18^de	28.76 ± 1.08^a	23.44 ± 2.68^b	5.40 ± 0.07^d	3.02 ± 0.09^de	1.48 ± 0.24^e	10.69 ± 0.77^c
78	150-84-5	23.07	Citronellyl acetate	26.23 ± 4.83^bc	22.86 ± 3.66^bc	24.37 ± 1.79^bc	15.38 ± 4.07^c	50.53 ± 12.99^a	18.84 ± 7.95^bc	32.85 ± 6.84^b
79	2051-49-2	16.72	Hexyl hexanoate	0.00 ± 0.00^e	2.77 ± 0.15^c	9.44 ± 1.23^a	1.56 ± 0.19^cd	4.97 ± 0.39^b	0.97 ± 0.05^de	1.18 ± 0.08^de
80	142-92-7	9.78	Hexyl acetate	0.00 ± 0.00^d	1.57 ± 0.27^cd	8.85 ± 1.48^a	2.79 ± 0.49^bc	4.39 ± 1.06^b	2.59 ± 0.17^bc	1.92 ± 0.54^c
81	120-51-4	22.56	Benzyl benzoate	2.17 ± 0.12^cd	4.02 ± 0.53^a	2.95 ± 0.53^bc	3.00 ± 0.45^bc	3.85 ± 0.42^b	1.96 ± 0.18^d	2.52 ± 0.27^cd
82	110-27-0	22.96	Isopropyl myristate	0.06 ± 0.01^e	2.55 ± 0.18^a	1.35 ± 0.12^bc	1.34 ± 0.07^bc	1.14 ± 0.07^cd	1.03 ± 0.03^d	1.44 ± 0.06^b
83	606-45-1	15.77	Methyl 2-methoxybenzoate	7.30 ± 0.20^a	0.56 ± 0.03^e	3.00 ± 0.26^b	2.02 ± 0.08^c	0.42 ± 0.03^e	1.01 ± 0.02^d	0.97 ± 0.11^d
84	102-16-9	14.94	Benzyl phenylacetate	0.97 ± 0.07^b	1.64 ± 0.04^a	1.07 ± 0.19^b	0.73 ± 0.07^c	0.38 ± 0.02^d	0.49 ± 0.02^d	0.74 ± 0.05^c
85	7011-83-8	16.72	Dihydrojasmone lactone	0.00 ± 0.00^e	3.14 ± 0.11^c	11.60 ± 1.36^a	1.65 ± 0.18^d	5.14 ± 0.36^b	1.36 ± 0.02^d	1.52 ± 0.11^d
86	25524-95-2	16.57	Jasmine lactone	5.34 ± 0.11^e	23.23 ± 0.92^d	76.66 ± 6.57^a	26.21 ± 1.94^d	22.16 ± 1.84^d	43.74 ± 1.33^b	36.1 ± 2.91^c
87	1211-29-6	23.24	Methyl jasmonate	12.81 ± 2.06^b	13.26 ± 2.07^b	12.50 ± 1.11^b	8.73 ± 1.89^b	27.01 ± 4.9^a	10.85 ± 4.47^b	15.19 ± 1.67^b
88	119-36-8	13.19	Methyl salicylate	294.81 ± 22.12^d	2,085.7 ± 131.83^a	307.14 ± 36.23^d	498.63 ± 15.53^c	764.22 ± 42.52^b	516.16 ± 30.26^c	483.65 ± 46.37^c
Esters (20)				504.25 ± 27.11^d	2,474.51 ± 157.38^a	1,740.43 ± 183.45^b	1,351.05 ± 114.32^c	1,342.27 ± 103.23^c	1,695.42 ± 61.37^b	1,591.61 ± 204.25^bc
All data are shown as the mean ± standard deviation (SD). Significant differences among various groups are represented by different letters (p < 0.05).. RT: retention time.

Table 1.

The volatile components of seven tea cultivars.

Volatile components	Aroma characteristics	Aroma thresholds^[3,27−30] (μg/kg)	Relative OAV value
Volatile components	Aroma characteristics	Aroma thresholds^[3,27−30] (μg/kg)	TGY	JX	FJSX	FDDB	BHZ	LJ43	SCZ
Geraniol	Rosy, sweet	7.5	181.19	680.45	402.37	343.19	31.96	176.24	334.77
Linalool	Floral, fruity	6	168.78	209.73	184.39	410.85	79.68	202.16	234.71
(E)-Nerolidol	Floral, citrus	15	3.65	3.65	4.84	2.27	7.16	4.47	7.67
Nerol	Rosy, orange	49	0.46	1.47	1.01	1.24	0.23	0.73	1.23
(Z)-3-Hexenol	Fresh, grassy	110	0.32	0.31	0.72	1.62	0.67	1.55	2.08
Phenylethyl alcohol	Floral, rosy	45	2.11	2.55	0.34	16.44	9.21	7.52	15.32
(Z)-Linalool oxide (furanoid)	Sweet, floral	190	0.14	0.52	0.23	1.35	0.45	1.07	0.69
(E)-Linalool oxide (furanoid)	Sweet, floral	190	0.43	1.66	0.43	3.51	1.00	2.15	1.57
Neral	Sweet, fruity	53	0.48	1.77	1.14	1.24	0.10	0.71	1.13
Decanal	Sweet, citrus	0.1	5.90	6.38	6.93	8.20	8.60	11.89	8.13
Heptanal	Fatty, citrus	10	14.31	0.22	0.22	0.16	0.12	0.11	0.09
Benzaldehyde	Almond, nutty	3	3.27	2.73	2.06	2.91	3.00	3.69	4.29
Phenylacetaldehyde	Woody, sweet	4	24.85	30.28	4.72	186.67	108.75	89.10	175.02
Hexanal	Fresh, fruity, fatty	4.5	1.74	3.43	2.77	4.14	6.13	6.55	4.99
Valeraldehyde	Almond, malty	12	1.20	1.74	1.12	2.02	2.79	2.59	3.35
Isovaleraldehyde	Fruity	4	12.22	5.28	8.30	9.25	8.59	11.41	9.79
α-Ionone	Violet, woody	0.4	30.13	58.20	36.75	44.53	6.50	25.85	35.27
β-Ionone	Violet, floral	0.07	1,245.71	1,299.60	1,327.56	1,478.32	1,589.21	1,549.00	1,631.16
Jasmone	Jasmine	7	0.95	0.68	0.61	0.74	1.36	1.12	1.78
β-Damascenone	Rosy	0.002	503.45	9,026.77	7,127.53	3,791.88	911.92	3,053.12	4,509.23
Indole	Floral	40	–	0.91	–	0.29	1.06	0.19	0.62
(E)-β-Ocimene	Floral, grassy	34	3.16	7.76	4.35	5.12	0.70	2.92	4.57
Myrcene	Fruity, balsamic	15	16.10	44.61	24.99	28.41	2.94	15.37	24.26
(E)-3-Hexen-1-ol acetate	Fruity, grassy	31	4.03	7.22	33.26	22.26	12.85	31.67	28.10
Methyl salicylate	Herbal, minty	40	7.37	52.14	7.68	12.47	19.11	12.90	12.09
Methyl jasmonate	Jasmine	3	4.27	4.42	4.17	2.91	9.00	3.62	5.06
'–' indicates that the OAV value cannot be calculated.

Table 2.

OAV values of differential volatile components in seven tea cultivars.