Genome-wide characterization and identification of candidate <i>CsRAV</i> genes involved in JA pathway responses to cold stress in tea plant (<i>Camellia sinensis</i>)

Figures (8) Tables (2)

Figure 1.
The chromosomal locations of CsRAV genes.
Figure 2.
Phylogenetic analysis of RAV genes from Camellia sinensis, Arabidopsis thaliana, and Oryza sativa.
Figure 3.
CsRAV protein and gene sequence analysis. (a) Phylogenetic tree analysis, the purple line represents group I, the green line represents group II and the yellow line represents group III. (b) Motif analysis. (c) Gene structure analysis. Horizontal lines indicate introns.
Figure 4.
Multiple alignments of CsRAV proteins in tea plants.
Figure 5.
Secondary structure prediction of the RAV family in tea.
Figure 6.
Predicted structures of conserved domains in RAV proteins of tea plants. (a) Ribbon diagrams of the B3 domains of the tea plant CsRAV proteins. (b) Ribbon diagrams of the AP2 domains of the tea plant CsRAV proteins.
Figure 7.
Cis-promoter elements of the CsRAV genes in tea plants. (a) The numbers of different cis-elements in the promoters of CsRAV genes are indicated in different colors in the grid. (b) The numbers of cis-elements in four functional categories per gene are represented by different colored histograms.
Figure 8.
The expression pattern of CsRAV genes in each sample. Error bars represent the standard error of the mean (n = 3). Different lowercase letters indicate significance at p < 0.05.

Treatment	Days 1–4	Days 5–8	Day 9
CK	Room temperature and water spray	Room temperature and water spray	Samples collected
T1	Room temperature and water spray	4 °C cold stress	Samples collected
T2	4 °C cold stress	Room temperature and water spray	Samples collected
T3	Room temperature and water spray	Room temperature and 100 μM MeJA spray	Samples collected
T4	Room temperature and 100 μM MeJA spray	Room temperature and water spray	Samples collected
T5	Room temperature and water spray	4 °C cold stress and 100 μM MeJA spray	Samples collected
T6	4 °C cold stress and 100 μM MeJA spray	Room temperature and water spray	Samples collected

Table 1.

Details of each treatment.

Number	Gene name	Gene ID	Amino acid residues	Molecular weight (kDa)	Theoretical pI	Grand average of hydropathicity	Subcellular localization
1	CsRAV1	TEA028514.1	366	40.67	8.96	−0.610	Nuclear
2	CsRAV2	TEA006376.1	375	41.78	9.30	−0.561	Nuclear
3	CsRAV3	TEA020682.1	329	36.77	8.97	−0.462	Nuclear
4	CsRAV4	TEA006600.1	344	39.68	6.20	−0.516	Nuclear
5	CsRAV5	TEA016614.1	352	39.56	8.19	−0.655	Nuclear
6	CsRAV6	TEA004203.1	359	41.07	5.61	−0.698	Nuclear
7	CsRAV7	TEA030533.1	1,130	127.04	5.20	−0.819	Nuclear

Table 2.

The physicochemical properties of tea plant RAV proteins.