Figures (4)  Tables (4)

    • Figure 1. 

      Wild fruiting body of Trametes versicolor on the living trunk of Tamarind (Tamarindus indica) tree.

    • Figure 2. 

      Mycelial growth of Trametes versicolor on different culture conditions. (a) Culture media (a1, SDA; a2, MEA; a3, PDA, a4, CWG; a5, RBDG, a6, PSG; a7, CGDG). (b) pH condition (b1, pH 5; b2, pH 6; b3, pH 7; b4, pH 8). (c) Aeration conditions (c1, Sealed; c2, Unsealed). (d) Illumination conditions (d1, Light; d2, Dark). (e) Temperature conditions (e1, Refrigerated; e2, Air conditioned; e3, Room temperature).

    • Figure 3. 

      Fruiting bodies of T. versicolor grown in 70% rice straw and 30% sawdust substrate formulation.

    • Figure 4. 

      Fitted regression line and equation to determine the lethal concentration (LC50) of T. versicolor fruiting body. (a) Ethanolic and (b) methanolic extract in the percentage mortality of brine shrimp nauplii.

    • Growth rate of
      mycelia (mm d−1)      		Mycelial
      density
      Intrinsic factorsCulture media
      CWG8.36 ± 0.94ab+++
      PSG3.80 ± 0.57d++
      CGDG3.93 ± 0.60d++
      RBDG5.91 ± 0.73cd+++
      PDA8.64 ± 1.18ab+++
      MEA10.27 ± 0.61a+++
      SDA6.64 ± 0.10bc+++
      pH
      5.07.69 ± 0.21b++++
      6.011.91 ± 4.52a++++
      7.014.26 ± 2.11a++++
      8.014.18 ± 2.19a++++
      AerationExtrinsic factors
      Sealed17.45 ± 0.6a++++
      Unsealed11.51 ± 0.23b++++
      Illumination
      Lighted16.97 ± 1.79a++++
      Dark13.36 ± 6.76b++++
      Temperature
      Refrigerated2.00 ± 0.00bNo growth
      Air-conditioned17.36 ± 1.12a++++
      Room temperature18.00 ± 0.00a++++
      Means with similar superscripts are statistically comparable from each other using Tukeys HSD and t-test at 5% level of significance. Coconut water Gulaman (CWG), Potato Sucrose Gulaman (PSG), Corn Grits Decoction Gulaman (CGDG), Rice Bran Decoction Gulaman (RBDG), Potato Dextrose Agar (PDA), Malt Extract Agar (MEA), Saboraud Dextrose Agar (SDA). Refrigerated condition (9 °C), Air-conditioned (25 °C), Room Temperature (32 °C). (+) very thin, (++) thin, (+++) thick, (++++) very thick.

      Table 1. 

      Mycelial growth of Trametes versicolor with different intrinsic and extrinsic factors.

    • Incubation period (d)Days of primordia initiation (d)Cap diameter (mm)Yield per bag (g bag−1)Biological efficiency (%)
      19.70 ± 0.4836.90 ± 14.7622.11 ± 3.5937.68 ± 15.707.54 ± 3.14

      Table 2. 

      Fructification parameters of Trametes versicolor.

    • MycochemicalsReactionFindings
      AlkaloidsTurbidity was formed+
      FlavonoidsLighter brown coloration*+
      GlycosidaseNo reaction−
      SteroidsNo reaction−
      SaponinsFrothing formation+
      TanninsBrownish with lighter green coloration+
      TerpenoidsBrown coloration interface+
      * Initial color of the extract was brown, thus positive yellow coloration results in a lighter brown. Note: (+) present; (−) absent.

      Table 3. 

      Mycochemical analysis of an aqueous extract of the fruiting body of T. versicolor.

    • ExtractConcentration
      (µg/ml)      		Mortality
      (%)      		LC50
      (µg/ml)      		Toxicity level*
      Ethanol00.00e70.93 Highly toxic
      116.67de
      1040.00cd
      10053.33bc
      1,00073.33ab
      10,00096.67a
      Methanol00.00d74.43Highly toxic
      110.00d
      1036.67c
      10046.67bc
      1,00066.67b
      10,00093.33a
      Means with similar superscripts are statistically comparable from each other using Tukey's HSD and t-test at 5% level of significance.
      * Toxicity level was based on the study of Mendoza et al.[28].

      Table 4. 

      Cytotoxic effect and LC50 values of ethanolic and methanolic extract of T. versicolor on brine shrimp nauplii after 24 h of exposure.