Figures (5)  Tables (2)

    • Figure 1. 

      Typical DNA comets are from anchovy and bluefish. Electrophoresis conditions: 2 V/cm for 2 min, propidium iodide staining. All slides were examined at a magnification of 20×.

    • Figure 2. 

      Changes in measured DNA comet parameters in anchovy samples.

    • Figure 3. 

      Changes in measured DNA comet parameters in bluefish samples.

    • Figure 4. 

      Dose dependent response curve of head diameter and tail moment for anchovy.

    • Figure 5. 

      Dose dependent response curve of head diameter and tail moment for bluefish.

    • SampleTreatment
      Number of treatment groupsAnchovyControl, 1.83, 2.85, 3.42, 4.22 kGy
      BluefishControl, 1.98, 3.93, 4.57, 5.40 kGy
      Number of samples for each groupAnchovy30
      Bluefish3
      Number of parallelsAnchovyControl
      1.83 kGy
      2.85 kGy
      3.42 kGy
      4.22 kGy      		3
      3
      3
      3
      3
      BluefishControl
      1.98 kGy
      3.93 kGy
      4.57 kGy
      5.40 kGy      		3
      3
      3
      3
      3

      Table 1. 

      Sampling and treatment plan.

    • Absorbed dose (kGy)Measured parameters (Mean ± Std. error)
      HDaHDNAbTDNAcTLdTMe
      AnchovyControl47.09 ± 2.0715.92 ± 1.8384.08 ± 1.8342.23 ± 3.6437.05 ± 3.40
      1.8316.35 ± 0.610.70 ± 0.0899.30 ± 0.08192.52 ± 1.72191.68 ± 1.74
      2.8519.00 ± 0.320.80 ± 0.0499.20 ± 0.04212.58 ± 1.34211.31 ± 1.35
      3.4213.36 ± 0.180.26 ± 0.0799.74 ± 0.07209.43 ± 3.13208.94 ± 3.14
      4.2210.77 ± 0.100.13 ± 0.0399.87 ± 0.03202.16 ± 1.34201.88 ± 1.35
      BluefishControl55.85 ± 1.5910.52 ± 0.5489.48 ± 2.0750.55 ± 0.5445.76 ± 2.22
      1.9817.29 ± 0.750.28 ± 0.0499.72 ± 5.39227.88 ± 0.04227.23 ± 5.39
      3.9312.91 ± 0.120.19 ± 0.0299.81 ± 2.15285.21 ± 0.02284.69 ± 2.15
      4.5712.12 ± 2.660.25 ± 0.1899.75 ± 3.15251.32 ± 0.18250.69 ± 3.49
      5.4011.07 ± 0.240.16 ± 0.0299.84 ± 2.13218.35 ± 0.02217.98 ± 2.13
      a HD: Head diameter. b HDNA: Head DNA. c TDNA: Tail DNA% = percent of DNA in the comet tail. d TL: Tail length = length of the comet tail measured from right border of head area to end of tail (micron = pixels). e TM: Tail moment = Tail DNA% × Tail length (DNA% in the tail).

      Table 2. 

      The mean values of measured parameters of comets derived from irradiated anchovy and bluefish.