Figures (7)  Tables (3)
    • Figure 1. 

      Phylogenetic analysis, protein motif structure, chromosome localization, and gene structure analysis of PmCBL genes. (a) Phylogenetic analysis of PmCBL genes. (b) Protein motif structure of PmCBL genes. (c) Chromosome localization analysis of PmCBL genes. (d) Gene structure analysis of PmCBL genes.

    • Figure 2. 

      Protein amino acid sequence comparison diagram. (a) Protein amino acid sequence comparison diagram between P. mume and A. thaliana. (b) Protein amino acid sequence comparison diagram between P. mume and V. vinifera. (c) Protein amino acid sequence comparison diagram between P. mume and O. sativa.

    • Figure 3. 

      Phylogenetic tree of P. mume, A. thaliana, O. sativa, N. tabacum and V. vinifera. The green checkmark represents V. vinifera, the black square represents O. sativa, the blue circle represents A. thaliana, the red star represents N. tabacum, and the purple triangle represents P. mume.

    • Figure 4. 

      Analysis of cis-acting elements in PmCBL genes.

    • Figure 5. 

      Expression patterns of PmCBL genes in different tissue parts of P. mume.

    • Figure 6. 

      The expression pattern of PmCBLs genes during overwintering. DD, November; NDD, December; LD, January; NF, February.

    • Figure 7. 

      Expression levels of PmCBL genes under 4 °C treatment.

    • Gene nameGene IDChromosomePositionSubcelllar localizationCDS (bp)Intron
      PmCBL1Pm000366Chr012287536~2289774Cell membrane2,4185
      PmCBL2Pm018367Chr0517870110~17871915Cell membrane1,8054
      PmCBL3Pm019754Chr0525555186~25557079Cell membrane1,8935
      PmCBL4Pm024130Chr0710584318~10585570Cell membrane1,2524
      PmCBL5Pm024134Chr0710621981~10623545Cell membrane1,5645
      PmCBL6Pm024135Chr0710622516~10628186Cell membrane2,4745

      Table 1. 

      Members of the P. mume PmCBL gene family and their main molecular characteristics and information.

    • Protein nameGene IDNumber of amino acidsMolecular weightThereotical pIInstability indexSignal peptide
      PmCBL1Pm00036622626052.714.8249.06NO
      PmCBL2Pm01836721724903.525.1435.97NO
      PmCBL3Pm01975421224429.824.7346.65NO
      PmCBL4Pm02413018621665.754.640.14NO
      PmCBL5Pm02413421324720.174.5740.98NO
      PmCBL6Pm02413521825144.714.8646.47NO

      Table 2. 

      Physicochemical properties of P. mume CBL gene family members.

    • GenePmCBL1PmCBL2PmCBL3PmCBL4PmCBL5PmCBL6
      Gene IDPm000366Pm018367Pm019754Pm024130Pm024134Pm024135
      CAAT-box346244
      CGTCA-motif21121
      TGACG-motif21121
      TATA-box444118
      GT1-motif1
      TCCC-motif1
      GATA-motif111
      ARE11
      G-box11
      MBS11
      TGA-element1
      A-box11
      I-box1
      O2-site1112
      LAMP-element1
      ABRE11
      TCA-element1
      CAAT-box was a common cis-acting element in the promoter and enhancer regions. CGTCA-motif/TGACG-motif was cis-acting regulatory elements involved in MeJA response. TATA-box was a core promoter element located around the transcription start site (-30). GT1-motif was a light-responsive element. TCA-element was a cis-acting element involved in salicylic acid response. TGA-element was an element involved in auxin response. ABRE was a cis-acting element involved in abscisic acid response. A-box was a cis-acting regulatory element. ARE was a cis-acting element required for anaerobic induction. G-box was a cis-acting element involved in light response. O2-site/MBS was cis-acting regulatory elements involved in zein protein metabolism regulation. TCCC-motif/GATA-motif/I-box/LAMP-element was part of light-responsive elements.

      Table 3. 

      Analysis of cis-acting elements in the P. mume CBL gene family members.